Assessment of RBCs membrane protective activity of citicoline and eicosapentanoic- decosahexanoic acid in osmotic fragility model
Keywords: osmotic fragility test, citicoline, EPA-DHA, hemolytic Anemia
AbstractDifferent organic compounds possess a good membranes stabilizing effects that protect cells like erythrocytes from strains exerted by change in medium osmolarity . These changes in tonicity could predispose RBCs for hemolysis . This disorder could be assessed by osmotic fragility test . From those compounds that widen osmotic fragility test values are citicoline (membrane phospholipids precursor) and polyunsaturated fatty acids EPA-DHA. Assessment of these compounds on osmotic fragility test revealed that both of citicoline and EPA-DHA will protect RBCs from hemolysis for a wider range than values of the control blank. Although these effects were statistically not significant on considering statistical correlation test, however, the differences were important on considering t test for the range of saline concentration between 0.4-0.5 at P<0.05 . From the overall results, both citicoline and pufa induced a significant change in statbility of RBCs membrane upon exposure of osmotic effects in the saline concentration between 0.4-0.5. Further evaluation of citicoline and EPA- DHA effects may be necessary before recommending the use these compounds in protection against hemolytic diseases.