Evaluation The Immunological State Of Womenâ€™s Sera Infected With Toxoplasmosis.
AbstractBackground: The ubiquitous protozoan parasite Toxoplasma gondii is a major cause of morbidity and mortality in neonates and immunocompromised hosts. Both acute invasion and reactivation of latent infection result in an inflammatory reaction with lymphocytes, macrophages, and neutrophils. Better understanding of the mechanisms of resistance of the host against this protozoan is important for development of safe, effective alternative treatment regimens for toxoplasmosis in the future.
Aim of study:This study aimed to investigate some immunological parameters of womanâ€™s sera infected with Toxoplasmosis in Baghdad city, through investigation the potential role of some interleukins mediating the inflammatory response after T. gondii infection.
Methods: Detection of parasite antibodies was achieved by MELA by testing 185 women which referred to central health laboratory during the period from March to July 2004; ELISA technique was used to detect serum concentration of IL-1Î±, IL-8, and IL-10 , also
SRID used to calculate total IgG,IgM, C3, and C4 concentrations in both group understudy.
Results:According to the study, there was significant increasing in mean concentration of IgG whereas IgM concentration do not affected in both patients and control groups, also there were no significant differences in the mean concentration of C3 and C4
component of complement between two groups of study. There were no significant differences in mean concentration of serum IL-1Î± (0.68 pg/ml) and IL-8 (20.14 pg/ml) in infected women when compared with healthy one (0.187 pg/ml) and (17.0 pg/ml)respectively. However, the mean serum concentration of IL-10 was highly and significantly elevated during T. gondii infection (21.125 pg/ml) in comparison to that of healthy control (9.525 pg/ml). The study did not record any significant change in the mean total WBCs count between patients and control groups, also there were no differences in mean percentage of neutrophils in both groups, the mean percentage of eosinophils and lymphocytes was highly significant in infected womenâ€™s samples as compared with control.
Conclusion:Most cases of toxoplasmosis were diagnosed at the chronic stags of disease, hence there were no clear sign and symptoms that draw attention, however, abortion was the only manifestation that refer to infection with toxoplasmosis, since the concentrations of pro inflammatory cytokines (like IL-1Î±) and chemokines (such as IL-8) decreased against increase the level of anti-inflammatory and regulatory cytokine IL-10 that prevent the host immune response leading to undesirable pathological changes.