Genotyping of Toxoplasma gondii isolates from human being in Wasit province / Iraq
Keywords: Toxoplasma gondii, Genotyping, RFLP, strain, human, Iraq
AbstractBackground: Toxoplasma gondii is an obligate intracellular protozoan parasite that infects almost all-warm blooded animals and human beings. No really data is known of genetic diversity of T. gondii in human in Iraq. The aim of this study was to determine the genotypes of T. gondii isolates from human in Wasit province, east Iraq.
Methods: A total of 508 clinical specimens (blood 500 and placenta 8) of women were initially examined serologically by ELISA, and further tested by RT-PCR technique through B1 gene amplification to confirm the infection with T. gondii. Then the positive DNA samples were assayed for genetic characterizations depend upon nested PCR-RFLP of SAG2 gene.
Results: Out of 508 tested samples only 15 were confirmed positive T. gondii DNA. The genotyping assay revealed that 6.6% (1/15), 13.3% (2/15) and 80% (12/15) of examined isolates represent the genotypes of I, III and II respectively.
Conclusion: The type II appeared is a dominant picture in human being in Wasit province / Iraq.