Molecular and Phylogenetic study of Klebsiella aerogenes isolated from infected burned skin

  • alaa Abdelkadhim Jawad Unit of Zoonotic Research, College of Veterinary Medicine, University of Al-Qadisiyah, Diwaniyah, Iraq
  • Hayder Naji Ayyez Unit of Zoonotic Research, College of Veterinary Medicine, University of Al-Qadisiyah, Diwaniyah, Iraq
  • Hayder Ali Muhammid Department of Microbiology, College of Vet. Medicine, Kerbala University
Keywords: Klebsiellaaerogenes, PCR, Sequencing, Phylogeny, Burn infections

Abstract

This study was intended to explore if infected burns of patients of Al-Diwaniyah City, Iraq, haveKlebsiellaaerogenes as one of the pathogenic bacteria that infect burns or wounds. For such case, traditional, Polymerase chain reaction (PCR), and DNA gyrase subunit B gene (gyrB)-partial sequencing tooling to confirm diagnosis. Primary testing of the sample using blood and MacConkey agars and certain biochemical tests alarmed that the bacterium was present in this specimen. The PCR results confirmed the occurrence of the infection by this bacterial agent. The gyrBgene-partial sequencing followed by drawing a phylogenic tree placed the bacterium in a separate cluster from other Enterobacteriaceae family members. The current study indicates that this bacterium is present in the infected burns or injuries in Al-Diwaniyahcity.Our results have been reviewed the current trends in molecular phylogenetic analysis, A computational phylogenetic study and data was approach and registered Klebsiellaaerogenes with accession number (MG560868.1).

Published
2019-08-25
Section
Articles