Isolation and Identification of Listeria .monocytogenes From Human and Animal in Al- Qadissiya Province

Abstract

The aim of this research is to detect about the infection in Listeria.monocytogenes germ in human and animals in Al-Qadissiya province , oxford listeria selective agar used for isolating L.monocytogenes germ , so the special stimulator for growth of the germ was added to it in order to determine the type of bacteria , also it diagnosed an isolates for L.monocytogenes germ by using the microscopical examination and biochemical tests , furthermore the germ isolates was planted on blood agar for diagnosing the blood analysis type: B-heamolysis as affirmative checking for the isolates .This study was carried out during the period from November 2014 to April 2015, whereas samples were collected from women that suffered of abortion and infected children in the maternal and children hospital in Al-Qadissiya province , so the samples were collected under supervising of a specialist medicine , the number of taken samples of the women is 65 samples , while the number of taken samples of children is 32 samples , so the samples that belong to animals have included a gall bladder samples were collected from Al-Diwaniya slaughterhouse were 100 samples of sheep and 100 samples of cows.in addition to collect 200 samples of milk as 100 samples for each sheep and cows from different rural zones in al- Qadissiya province. The results of the research showed 9 isolates for L.monocytogenes germ have been isolated from human samples in percentage 9.27% of total amount of the human samples , whereas the isolation percentage in women infected in abortion is 4.61%, while the percentage of listeria germ isolation was higher in the children that infected in meningitis whereas it was 18.75%.As for the percentage of the isolating in sheep it was 4% and 7% for milk and gall bladder respectively. in cows the isolation percentage of the germ from milk and gall bladder was 2% and 3% respectively . So the the results of isolating and diagnosing listeria germ during the study period in human and animals were disparate , whereas the highest percentage of isolation were scored in the cold months of the year. We can conclude of the research results is that the percentage of infection in L.monocytogenes germ was high in human in al-Qadissiya province , in which refer to the risks of the diseases and health problems spread that could be caused by L.monocytogenes germ , also the results included isolating the germ from milk and gall bladder samples for sheep and cows that could be a source to carry the infection to the human by contaminating the meat, milk and its derivatives