A Identification and Isolation of Several Bacteria from oral cavity among human in Soran City

Identification and Isolation of Several Bacteria in Soran City

  • Holem Balaky General Science Department, Faculty of Education, Soran University, Erbil, Iraq
  • Alaa Taha Younis Al-Hammadi Department of Microbiology, College of Education for Pure Science, University of Mosul, Mosul, Iraq https://orcid.org/0000-0003-1740-1145
Keywords: Bacteria, Oral Cavity, Human, Antibiotics, Soran City

Abstract

The aim of this study has been to estimate that the number of bacteria in the mouth exceeds the total number of people on the planet. In this context, scientists have discovered more than 700 different types of human oral bacteria. With such a large number of oral bacteria present, many diseases that may threaten health, especially oral health, can emerge. For this reason, this study was employed and conducted on 252 healthy people in the Soran area of the city of Erbil. Both sexes were involved and different ages were selected. Swabs taken from 226 participants gave positive growths on different culture media. The biochemical properties of the isolates were tested according to Bergeys Manual of Systematic Bacteriology and UK Standards for Microbiology Investigation. The results showed that Staphylococcus aureus was the most frequent isolated pathogen (47.83%) followed by Pseudomonas aeruginosa (16.30%), Bacillus subtilis (23.91%), Enterococcus faecalis (5.43%), Enterobacter aerogenes (4.35%), and Klebsiella pneumonia (2.17%) respectively. Antibiotic Susceptibility testing of different species of bacteria was also performed according to Kirby Bauer disc diffusion method on Muller Hinton Agar by using commercial antibiotic discs. It seems that the different species of bacteria showed various sensitivity patterns to several kinds of antibiotics.

Downloads

Download data is not yet available.

References

Aas, J.A., Paster, B.J., Stokes, L.N., Olsen, I., and Dewhirst, F.E., 2005. Defining the normal bacterial flora of the oral cavity. Journal of Clinical Microbiology, 43 (11), 5721–5732.
Al-Abdul AA, H.I., 2017. Isolation and Identification of Bacteria from Diabetic and Non-diabetic Patients with Periodontitis. Donn J Microb Biotec Res, 4 (2), 004–009.
Barkvoll, P. and Attramadal, A., 1989. Effect of nystatin and chlorhexidine digluconate on Candida albicans. Oral Surgery, Oral Medicine, Oral Pathology.
Cuesta, A.I., Jewtuchowicz, V., Brusca, M.I., Nastri, M.L., and Rosa, A.C., 2010. Prevalence of Staphylococcus spp and Candida spp in the oral cavity and periodontal pockets of periodontal disease patients. Acta odontológica latinoamericana : AOL, 23 (1), 20–26.
Do, T., Devine, D., and Marsh, P.D., 2013. Oral biofilms: molecular analysis, challenges, and future prospects in dental diagnostics. Clinical, cosmetic and investigational dentistry, 5, 11.
Dolnicar, S. and Chapple, A., 2015. The human oral microbiome. Annals of Tourism Research.
Hua Xie, H.X., Cook, G.S., Costerton, J.W., Bruce, G., Rose, T.M., and Lamont, and R.J., 2000. Intergeneric Communication in Dental Plaque Biofilms.
Lateef, B. and Ibrahim, V., 2017. Isolation and Identification Some of microbial causes for dental caries. Tikrit Journal of Pure Science, 22 (9), 9–12.
Marcotte, H. and Lavoie, M.C., 1998. Oral Microbial Ecology and the Role of Salivary Immunoglobulin A. Microbiology and Molecular Biology Reviews, 62 (1), 71–109.
Ohara-Nemoto, Y., Haraga, H., Kimura, S., and Nemoto, T.K., 2008. Occurrence of staphylococci in the oral cavities of healthy adults and nasal-oral trafficking of the bacteria. Journal of Medical Microbiology, 57 (1), 95–99.
P.Tille, 2014. Bailey & Scott’s Diagnostic Microbiology-E-Book.
Petti, S., M, B., GA, M., C, P., and A, P., 2014. High salivary Staphylococcus aureus carriage rate among healthy paedodontic patients.
Press, W. ~H., Flannery, B. ~P., and Teukolsky, S. ~A., 2004. Textbook of clinical cariology. Convergence in the information industries. Telecommunications, broadcasting and data processing 1981-1996.
Y Li, Y Ge, D.S., 2007. Genetic profiling of the oral microbiota associated with severe early-childhood caries.
Published
2021-09-14
How to Cite
Balaky, H., & Alaa Taha Younis Al-Hammadi. (2021). A Identification and Isolation of Several Bacteria from oral cavity among human in Soran City. Al-Qadisiyah Journal of Pure Science, 26(5), Bio 1-7. https://doi.org/10.29350/qjps.2021.26.5.1384
Section
Biology