Detection of Human Respiratory Syncytial Virus Associated with Asthmatic Patients Using Tissue culture and Real time – Polymerase chain reaction

Abstract

This study aimed to determine incidence human respiratory syncytial virus (hRSV) isolated from patients with asthma in Wasit province, Iraq. Blood samples were collected for measurement total and differential white blood cells (WBCs). Results showed total of WBCs and differential were significant (P<0.001) correlation in neutrophils, lymphocytes, eosinophils and basophils counts among different groups studied. Enzyme linked immunosorbent assay (ELISA) technique has been applied for detection of total-IgE antibodies. Results revealed that the highest total-IgE antibodies titer in sera were significantly difference (p < 0.01).. A total of 80 nasopharyngeal swabs were immediately dipped in transport media and stored until using for the detection of suspected hRSV patients group were used in real time – polymerase chain reaction (RT-PCR) from which 15 samples (18.75 %) out of 80 samples gave positive result for this test. The isolated hRSV 9 samples (11.25%) was cultured successfully on HeLa cell line, Cervical human carcinoma, as there were characteristic cytopathic effects observed in monolayer of cells line. All positive samples in tissue culture were confirmed by RT-PCR which indicated that the samples were positive for hRSV indicate cultured of it. The present of the virus in patients with asthma.