Evaluation of the ability of some bacterial species isolated from UTIto form bio film
Abstract
Urinary tract infection is one of the most serious infectious disease in the world , This study aimed to isolate and diagnose the bacteria that cause UTI and then evaluate its ability to form a biofilm. 100 urine samples were collected for a group of patients attending Al-Diwaniyah Teaching Hospital, who were confirmed to have UTI at different ages. The results showed the presence of bacteria. Escherichia coli and proteus spp in all samples(100%), while Staphylococcus aureus was found in 87% of samples , Enterobacter spp was isolated by 67% and Pseudomonas aeruginosa by 65%. Klebsiella pneumonia at 50 % and Staphylococcus epidermidis and Micrococcus spp each with 30%. Regarding the ability of the isolated bacteria to form biofilm, two methods used Congo-red agar and tube method and the results were as follows 64% for E.coli, K pneumonia with 66.6% while S. aureus showed the ability of formation with 41.6% and negative results for the rest by using tube method while Congo-red agar method results were 25% for 50% for E.coli and S. aureus. The study concluded that Congo red agar method is easy to perform and interpret, while the tube method is highly sensitive and specific.
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References
Clin. Microbiol. Rev., 15(2),167-193.
2. Gunardi, Wani Devita, (2021)"Biofilm-Producing Bacteria and Risk Factors (Gender and Duration of Catheterization) Characterized as Catheter-Associated Biofilm Formation." International Journal of Microbiology .
3. Prasad, S., Nayak, N., Satpathy, G., Nag, H. L., Venkatesh, P., Ramakrishnan, S., & Nag, T. C. (2012). Molecular & phenotypic characterization of Staphylococcus epidermidis in implant related infections. The Indian journal of medical research, 136(3), 483.
4. Murray, P. R., Rosenthal, K. S., & Pfaller, M. A. (2020). Medical microbiology E-book. Elsevier Health Sciences.
5. Dunne, W.M.; Jr. (2002). Bacterial adhesion: Seen any good biofilms lately? Clin. Microbiol. Rev., 15(2),155-166.
6. Hola, V.; Ruzicka, F. (2011). The formation of poly-microbial biofilms on urinary catheters. In: Urinary Tract Infections. Take, P. InTech, Croatia.
7. Petersen, J., & McLaughlin, S. (2016). Laboratory exercises in microbiology: Discovering the unseen world through hands-on investigation.
8. Sampaio, J., Machado, D., Gomes, A. M., Machado, I., Santos, C., Lima, N., & Martins, M. (2016). Deciphering
the contribution of biofilm to the pathogenesis of peritoneal dialysis infections: characterization and microbial behavior on dialysis fluids. PloS one, 11(6), 0157870.
9. Hancock, V.; Dahl, M.; Klemm, P. (2010). Abolition of biofilm formation in urinary tract Escherichia coli
and Klebsiella isolates by metal interference through competition for. Fur. Appl. Environ. Microbial., 76(12), 3836-3841.
10. Hola, V.; Ruzicka, F.; Votava, M. (2006). The dynamics of Staphylococcus epidermis biofilm formation about nutrition, temperature, and time. Scripta Medica (BRNO), 79(3),169-174.
11. Hassan, A.; Usman, J.; Kaleem, Fatima.; Omair, M.; Khalid, A.; Iqbal, M. (2011). Evaluation of different detection methods of biofilm formation in the clinical isolates. Braz. J. Infect. Dis., 15(4), 305-311.
12. Parsek, M.; Singh, P. (2003). Bacterial biofilms: An emerging link to disease pathogenesis. Annu. Rev. Microbiol., 57, 677-701.
13. Kumari, P., Mishra, R., Arora, N., Chatrath, A., Gangwar, R., Roy, P., & Prasad, R. (2017). Antifungal and anti-biofilm activity of essential oil active components against Cryptococcus neoformans and Cryptococcus laurentii. Frontiers in microbiology, 8, 2161.
14. Bonkat, G., Pickard, R., Bartoletti, R., Bruyère, F., Geerlings, S., Wagenlehner, F., ... & Veeratterapillay, R. (2018). Urological infections. Arnhem: European Association of Urology.
15. Oliveira, A.; Cunha, M.L. (2010). Comparison of methods for the detection of biofilm production in coagulase-negative Staphylococci. BMC. Res. Notes., 3, 260.
16. Khan, F.; Shukla, I.; Rizvi, M.; Mansoor, T.; Sharma, S.C. (2011). Detection of biofilm formation in Staphylococcus aureus. does it have a role in the treatment of MRSA infections?. Trends. Med. Res., 6(2),116-123.
17. Havaei, S. A., Moghadam, S. O., Pourmand, M. R., & Faghri, J. (2010). Prevalence of genes encoding bi-component leukocidins among clinical isolates of methicillin-resistant Staphylococcus aureus. Iranian journal of public health, 39(1), 8.
18. Hola, V.; Ruzicka, F.; Horka, M. (2010). Microbial diversity in biofilm infections of the urinary tract with the use of sanitation techniques. FEMS Immunol Med Microbiol., 59, 525-528.
19. Lewis, K. (2001). Riddle of biofilm resistance. Antimicrobial Agents and Chemotherapy,45, 999-1007.
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