Preparation and Determination of Nickel (II), Copper (II) and Cobalt (II) with new organic reagent by spectrophotometric methods

Abstract

A simple, accurate and sensitive spectrophotometric method for the determination of Nickel (II), Copper (II) and Cobalt (II) is described. The method is based on the chelation of these ions with 5-((4'-amino-3,3'-dimethyle-[1,1'-biphenyle]-4-yl)azo)-2-hydroxy benzoic acidص(ADBHB) that show maximum absorption at 370 nm to form an intense colour soluble products, that are stable and have a maximum absorption at 443, 455 and at 472 nm respectively. The molar extinction coefficient (ε) 4.2×104, 1.7×104 and 1.3×103 L.mole-1.cm-1 for Ni (II), Cu (II) and Co (II) respectively .The stability constant, relative error and standard deviations for these ions were, 4.4×107, 2.5×106 and 1.3×106 L.mole-1 and (7×10-4, 0.37%), (1.2×10-3, 0.7%) and (8.6×10-4, 0.4%) respectively. The new reagent and the three metal complexes have been prepared in aqueous solutions and were characterized by electronic spectrum, F.T.IR. Under optimum conditions the described methods were applied to find the biological activity of these complexes with Erwinia caratovora and Xanthomonas sp. Bacteria.