Estimation of the total lipids concentration and fatty acids diagnosis in the tapeworm parasite Postgangesia hemispherous parasitic in European Fishes Silurus glanis from Tigris River-Mousl city
Keywords:
Postgangesia hemispherous, Silurus glanis, Lipids,fatty acids
Abstract
Concentration of total lipids in each of the tapeworm Postgangesia hemispherous and intestine of its final host, normal and infected European fish Silurus glanis, were estimated. The analysis and diagnosis of fatty acids in the tapeworm have also been studied using Gas- chromatography (GC). The total concentration of lipid in tapeworm tissues was (148.41±0.8) mg/ 100 ml of worm extract, while in intestine of normal and infected final host were (168.003±1.21) and (107.04±1.2) mg/ 100 ml of gut extract respectively. A significant difference between the host, normal and infected fishes was observed at probability level of (p ≤ 0.05). According to retention time of standard esterified fatty acids, the analysis of fatty acids of the tapeworm have showed six fatty acids involved two saturated fatty acids (Plamitic and Stearic acids), three unsaturated fatty acids (Oleic, Linoleic and Linolenic acids), and one unknown fatty acid which was detected but not identified because of the Gas- chromatography has no more than five standard fatty acids. The major fatty acid was the saturated Palmitic one with 69.2 % among the other fatty acids.Downloads
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References
[1] اØمد, طارق يونس والهلالي,عبد الكريم عبد علي 2010 ,الكيمياء الØياتية. الجزء الثاني. دار ابن الاثير للطباعة والنشر, جامعة الموصل ,العراق.1-360.
[2] النجÙÙŠ, طلال سعيد ,1987 الكيمياء الØياتية. دار الكتب للطباعة والنشر, جامعة الموصل, العراق.1-395.
[3] الجلبي, قصي عبد القادر ,عبد, بيداء Øازم وسليم, خولة يونس 1985, مقدمة ÙÙŠ الكيمياء الØياتية لللبيدات. مديرية مطبعة الجامعة, جامعة الموصل ,العراق.1-367.
[4] Mills, G.L.; Taylor, D.C. and Williams J.F. (1981). Lipid composition of metacestodes of Taenia taeniaeformis and lipid during growth. Molecular and Biochemical Parasitology, 3 (5): 301–318.
]5[ Persat, F.; Mojon, M. and Petary, A.F. (1988). Lipids of Echinococcus multilocularis metacestodes. Comparative Biochemistry and Physiology, 91 (1): 133–136.
[6] Aisien, S.O. and Ogiji, E.E. (1989).Observation on the lipids of Oochoristica agamae (Cestoda). Parasitology Research, 75 : 307– 310.
[7] Mondal, M.; Mukhopchyay, D.; Ghosh, D.; Dey, C. and Misra, K.K. (2009). Analysis of Major lipid classes and their fatty acids in cestode parasite domestic fowle, Raillietina (Fuhrmannetta ) echinobothrida. Proceeding of Zoological Society, 62 (2): 131–137. ]
[8]Pappas, P.W. and Narcisi, E.M. (1982). A comparison of membrane–bound enzymes, the isolated brush border plasma membranes of the cestodes Hymenolepis diminuta and H. microstoma. Parasitology, 84: 391–396.
[9] Tietz, N.W. (1990). "Textbook of clinical chemistry". W.B. Saunders company,USA.
[10] Al-Kaisy,M.T.;Hadwan,H.A.;Humoud,m. and Hussen,A.K.(1991). Determination of citric acid and oxalic acid in fermented solutions of Aspergillus niger by Gas-Liquid Chromatography. Iraqi. J.Microbiol., 3(1):170-174.
[11] Stoffel,W.;Chu,F.and Ahrens, E.H.J.(1959). Analysis of long chain fatty acids by gas liguid chromatography, micromethode for preparation of methylesters. Journal of Analytical Chemistry,31:310-307
[12] القرشي, اØسان كاظم شري٠2007, الطرائق المعلمية والطرائق اللامعلمية ÙÙŠ الاختبارات الاØصائية, الطبعة الاولى. بغداد. 1-288.
[13] Sonue, M.B. (2012). Biochemical studies of gastrointestinal cestode parasites in Ovis bharal (L.) from Vidharbha region. Bioscience Discovery, 3(3):321–322.
[14]Jadhav, B.V.; Singh, S.P.; Bhure, D.B. and Padwal, N.D. (2008). Biosystematic studies of Davainea shindei n.sp. (Cestoda Davainidae ) Fuhrmann, 1907 from Gallus gallus domesticus. National Academy Science Letters,31(7): 245–250.
[15]Jawale, S.; Fartade, A. and Borde, S. (2011). Biochemical studies of caryophyllidean tapeworms in freshwater fish Clarias batrachus. Recent Research in science and Technology, 3(3):35–36.
[16] Hassan, H.F.; Hashim, D.S. and Abdullah, S.M.A. (2016). Identification of some Iraqi fish parasites by using biochemical and molecular protocols. International Journal of Current Research and Academic Review, 4(1): 54-64.
[17] Obal, G.; Ramos , A.L.; Silva, V.; Lima, A.; Batthyany, C. and Bessio, M.I. (2012). Characterization of the native lipid Moiety of Echinococcus granulosus antigen B. Plos Neclected Tropical Diseases, 6 (5): 1–11.
[18] النÙطجي, منى طاهر Ù…Øمد ,2006 دراسة نسجية وكميوØيوية لبعض الديدان الشريطية ÙÙŠ مضائ٠Ùقرية مختلÙØ©. اطروØØ© دكتوراه , علوم Øياة, كلية العلوم , جامعة الموصل, العراق.1-216.
[19] المولى ,سالي اØمد ذياب يونس ,2010 المتغيرات الكيموØيوية ÙÙŠ عدد من الديدان الخيطية التي تصيب الÙقريات. اطروØØ© دكتوراه, علوم Øياة , كلية العلوم, جامعة الموصل,العراق1.-229.
[20] Sood, M.I. (2006). Histochemical, biochemical and immunological studies in Haemonchus contortus (Nematoda: Trichostrongy–Ioidea an Indian perspective. Journal of Parasitic Diseases, 30 (1): 4–15.
[21]Sive Sai Kumari, M.R. (1994). Total lipid content in the cestode Neokrimia singhia parasitic in Peridicula astatica. Revista di Parasitologia, XI (LV) (3): 339–343
[22]Smyth,J.D.(1994)."Introduction to animal parasitology". Cambridge University Press.
[23] Vargas, R. and Bessonart, M. (2007). Lipid body composition of black catfish, Rhamdia quelen (Siluriformes, Heptapteridae) of two populations adapted to environmental conditions. Boletim do Instituto de Pesca- Governodo Estado de Säo Paulo,33(1):105-111.
[24]Cirković, M.; Ljubolević, D.; Dordević , V. Novakov, N.; petrovijević, R. Matekalo-Sverak, V. and Trbović, D. , (2012). The breed effect on productivity and meat nutrient compsition of fish. Kafkas Universitesi Veteriner Fakultesi Dergisi, 18 (5): 775–780.
[25] Ljubojević, D.; Ćirković, M.; Dordević, V.; Puraća, N.; Trbović, D.; Vukadinor, J. and Plavsa , N. (2013). Fat quality of marketable freshwater fish species in the Republic of Serbia. Czech Journal of Food Science, 31: 445 – 450.
[26]Miroslav , C.; Dejana ,T.; Dragana, L. and Vesna, D. (2011). Meat quality of fish farmed in polyculture in carp ponds in Republic of Serbia. Technologija Mesa, 52 (10) : 106–121.
[27]Stancheva, M.; Merdzhanova, A.; Dobreva, D.A. and Makedonski, L. (2014). Common carp (Cyprinus carpio) and European calfish (Siurus glanis) from the Danube River as Sources of fat soluble vitamins and fatty acids. Czech Journal of Food Sciences, 32 (1): 16–24.
[28]Fajmonova,E.;Zelenka,J.;Komprda,T.;Kladroba,D. and Sarmanova,I.(2003). Effect of sex,growth intensity and heat treatment on fatty acid composition of common carp (Cyprinus carpio) fillets. Czech Journal of Animal Science,48:85-92.
[29] Liu, X.L.; Serhan, C. N. and weller, P.F. (1990). Intravascular filarial parasites elaborate cyclooxygenase– derived eicosanoids. The Journal of Experimental Medicine, 172 : 993 – 996.
[30]Harris,W.S.(2004). Fish oil supplementation: Evidence for health benefits. Cleveland Clinic Journal of Medicine,71(3):221-226..
[31]Tocher, D. (2003). Metabolism and functions of lipid and fatty acids in teleost fish. Reviews in Fisheries Science,11:107-184.
[2] النجÙÙŠ, طلال سعيد ,1987 الكيمياء الØياتية. دار الكتب للطباعة والنشر, جامعة الموصل, العراق.1-395.
[3] الجلبي, قصي عبد القادر ,عبد, بيداء Øازم وسليم, خولة يونس 1985, مقدمة ÙÙŠ الكيمياء الØياتية لللبيدات. مديرية مطبعة الجامعة, جامعة الموصل ,العراق.1-367.
[4] Mills, G.L.; Taylor, D.C. and Williams J.F. (1981). Lipid composition of metacestodes of Taenia taeniaeformis and lipid during growth. Molecular and Biochemical Parasitology, 3 (5): 301–318.
]5[ Persat, F.; Mojon, M. and Petary, A.F. (1988). Lipids of Echinococcus multilocularis metacestodes. Comparative Biochemistry and Physiology, 91 (1): 133–136.
[6] Aisien, S.O. and Ogiji, E.E. (1989).Observation on the lipids of Oochoristica agamae (Cestoda). Parasitology Research, 75 : 307– 310.
[7] Mondal, M.; Mukhopchyay, D.; Ghosh, D.; Dey, C. and Misra, K.K. (2009). Analysis of Major lipid classes and their fatty acids in cestode parasite domestic fowle, Raillietina (Fuhrmannetta ) echinobothrida. Proceeding of Zoological Society, 62 (2): 131–137. ]
[8]Pappas, P.W. and Narcisi, E.M. (1982). A comparison of membrane–bound enzymes, the isolated brush border plasma membranes of the cestodes Hymenolepis diminuta and H. microstoma. Parasitology, 84: 391–396.
[9] Tietz, N.W. (1990). "Textbook of clinical chemistry". W.B. Saunders company,USA.
[10] Al-Kaisy,M.T.;Hadwan,H.A.;Humoud,m. and Hussen,A.K.(1991). Determination of citric acid and oxalic acid in fermented solutions of Aspergillus niger by Gas-Liquid Chromatography. Iraqi. J.Microbiol., 3(1):170-174.
[11] Stoffel,W.;Chu,F.and Ahrens, E.H.J.(1959). Analysis of long chain fatty acids by gas liguid chromatography, micromethode for preparation of methylesters. Journal of Analytical Chemistry,31:310-307
[12] القرشي, اØسان كاظم شري٠2007, الطرائق المعلمية والطرائق اللامعلمية ÙÙŠ الاختبارات الاØصائية, الطبعة الاولى. بغداد. 1-288.
[13] Sonue, M.B. (2012). Biochemical studies of gastrointestinal cestode parasites in Ovis bharal (L.) from Vidharbha region. Bioscience Discovery, 3(3):321–322.
[14]Jadhav, B.V.; Singh, S.P.; Bhure, D.B. and Padwal, N.D. (2008). Biosystematic studies of Davainea shindei n.sp. (Cestoda Davainidae ) Fuhrmann, 1907 from Gallus gallus domesticus. National Academy Science Letters,31(7): 245–250.
[15]Jawale, S.; Fartade, A. and Borde, S. (2011). Biochemical studies of caryophyllidean tapeworms in freshwater fish Clarias batrachus. Recent Research in science and Technology, 3(3):35–36.
[16] Hassan, H.F.; Hashim, D.S. and Abdullah, S.M.A. (2016). Identification of some Iraqi fish parasites by using biochemical and molecular protocols. International Journal of Current Research and Academic Review, 4(1): 54-64.
[17] Obal, G.; Ramos , A.L.; Silva, V.; Lima, A.; Batthyany, C. and Bessio, M.I. (2012). Characterization of the native lipid Moiety of Echinococcus granulosus antigen B. Plos Neclected Tropical Diseases, 6 (5): 1–11.
[18] النÙطجي, منى طاهر Ù…Øمد ,2006 دراسة نسجية وكميوØيوية لبعض الديدان الشريطية ÙÙŠ مضائ٠Ùقرية مختلÙØ©. اطروØØ© دكتوراه , علوم Øياة, كلية العلوم , جامعة الموصل, العراق.1-216.
[19] المولى ,سالي اØمد ذياب يونس ,2010 المتغيرات الكيموØيوية ÙÙŠ عدد من الديدان الخيطية التي تصيب الÙقريات. اطروØØ© دكتوراه, علوم Øياة , كلية العلوم, جامعة الموصل,العراق1.-229.
[20] Sood, M.I. (2006). Histochemical, biochemical and immunological studies in Haemonchus contortus (Nematoda: Trichostrongy–Ioidea an Indian perspective. Journal of Parasitic Diseases, 30 (1): 4–15.
[21]Sive Sai Kumari, M.R. (1994). Total lipid content in the cestode Neokrimia singhia parasitic in Peridicula astatica. Revista di Parasitologia, XI (LV) (3): 339–343
[22]Smyth,J.D.(1994)."Introduction to animal parasitology". Cambridge University Press.
[23] Vargas, R. and Bessonart, M. (2007). Lipid body composition of black catfish, Rhamdia quelen (Siluriformes, Heptapteridae) of two populations adapted to environmental conditions. Boletim do Instituto de Pesca- Governodo Estado de Säo Paulo,33(1):105-111.
[24]Cirković, M.; Ljubolević, D.; Dordević , V. Novakov, N.; petrovijević, R. Matekalo-Sverak, V. and Trbović, D. , (2012). The breed effect on productivity and meat nutrient compsition of fish. Kafkas Universitesi Veteriner Fakultesi Dergisi, 18 (5): 775–780.
[25] Ljubojević, D.; Ćirković, M.; Dordević, V.; Puraća, N.; Trbović, D.; Vukadinor, J. and Plavsa , N. (2013). Fat quality of marketable freshwater fish species in the Republic of Serbia. Czech Journal of Food Science, 31: 445 – 450.
[26]Miroslav , C.; Dejana ,T.; Dragana, L. and Vesna, D. (2011). Meat quality of fish farmed in polyculture in carp ponds in Republic of Serbia. Technologija Mesa, 52 (10) : 106–121.
[27]Stancheva, M.; Merdzhanova, A.; Dobreva, D.A. and Makedonski, L. (2014). Common carp (Cyprinus carpio) and European calfish (Siurus glanis) from the Danube River as Sources of fat soluble vitamins and fatty acids. Czech Journal of Food Sciences, 32 (1): 16–24.
[28]Fajmonova,E.;Zelenka,J.;Komprda,T.;Kladroba,D. and Sarmanova,I.(2003). Effect of sex,growth intensity and heat treatment on fatty acid composition of common carp (Cyprinus carpio) fillets. Czech Journal of Animal Science,48:85-92.
[29] Liu, X.L.; Serhan, C. N. and weller, P.F. (1990). Intravascular filarial parasites elaborate cyclooxygenase– derived eicosanoids. The Journal of Experimental Medicine, 172 : 993 – 996.
[30]Harris,W.S.(2004). Fish oil supplementation: Evidence for health benefits. Cleveland Clinic Journal of Medicine,71(3):221-226..
[31]Tocher, D. (2003). Metabolism and functions of lipid and fatty acids in teleost fish. Reviews in Fisheries Science,11:107-184.
Published
2018-01-27
How to Cite
Al-Niaeemi, B. H. S., & Mikael, M. H. (2018). Estimation of the total lipids concentration and fatty acids diagnosis in the tapeworm parasite Postgangesia hemispherous parasitic in European Fishes Silurus glanis from Tigris River-Mousl city. Al-Qadisiyah Journal of Pure Science, 22(3), 166 - 174. Retrieved from https://journalsc.qu.edu.iq/index.php/JOPS/article/view/603
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