Dissemination of some Antibiotic Resistance in Pseudomonas aeruginosa Isolates in Al-Diwaniya Hospitals.

  • Syoof Khowman Alramahy Biology Department - College of Sciences - University of Al-Qadisiyah
  • Zainab Falh Aladily Biology Department - College of Sciences - University of Al-Qadisiyah


   Pseudomonas aeruginosa  is one  of the primary opportunistic pathogens responsible  for nosocomial infections. Aminoglycosides and quinolones are an important component of antipseudomonal chemotherapy. The inactivation of aminoglycosides by modifying enzymes and 16S rRNA methylase are the most common mechanisms of aminoglycoside resistance , As for The inactivation of quinolones by occurrence of mutation encoded in the genes for the production of DNA gyrase  and Topoisomerase-IV enzymes .The goal of the present study was to investigate the occurrence of aminoglycoside and quinolones resistance and the incidence of important modifying enzyme and 16S rRNA methylase genes in P. aeruginosa isolates in Al-Diwaniya Teaching Hospital.                                                                                                                
 The samples of the study were collected from various sources and they were 350 samples from ear, burn, sputum, and urinary tract infections were examined for the detection of P. aeruginosa in Al-Diwaniya Teaching Hospital for the period from November 2014 to March 2015, the results of cultural and biochemical tests showed that 50 isolate belong to      P. aeruginosa and their diagnosis were confirmed by API-20E .                                               The antibiotic resistance profiles of the all isolates were determined by disk diffusion method of Kirby-Bauer against six antipseudomonal agents belonging to two classes. A total of 50 (14.2%) isolates of   P. aeruginosa were identified in the study period . Thirty-two (64%) isolates were found to be resistant to at least one aminoglycosides tested; with gentamicin being the highest (64%) and amikacin (26%) the lowest. Moderate resistance rates were exhibited against ciprofloxacin (20%) and norfloxacin (14%).                                                                                                                                                                                                                 


[1] Gawish, A.; Mohammed, N.; El-Shennawy, G. and Mohammed, H. (2013). An investigation of type 3 secretion toxins encoding-genes of Pseudomonas
aeruginosa isolates in a university hospital in Egypt. J. of Microbio. and Infec. Dise, 3 (3): 116-122.

[2] Cicek, A.C.; Saral, A.; Duzgun, A.O.; Cizmeci, Z.; Kayman, T.; Balci, P.O.; Dal, T; Firat, M.; Yazici, Y.; Sancaktar, M.; , Osman Birol Ozgumus, O.B. and Sandalli, C. (2013). Screening of class 1 and class 2 integrons in clinical isolates of Pseudomonas aeruginosa collected from seven Hospitals in Turkey: A Multicenter Study. J. Med. Microb., 3:227-233 .

[3] Hamed, S.M.; Aboshanab, K.M.A.; Walid F. Elkhatib, W.F. and Ashour, M.S. (2013). Aminoglycoside resistance patterns of certain Gram negative uropathogens recovered from hospitalized Egyptian patients. British Microbiol. Rese. J., 3(4): 678-691.

[4] Laureti, L.; Matic, I. and Gutierrez, A. (2013). Bacterial responses and genome instability induced by subinhibitory concentrations of antibiotics. Antibiotics., 2:100-114.

[5] Vaziri, F.; Peerayeh, S.N.; Nejad, Q.B. and Farhadian, A. (2011). The prevalence of aminoglycoside-modifying enzyme genes (aac (6')-I, aac (6')-II, ant(2')-I, aph(3')-VI) in Pseudomonas aeruginosa. Clinics., 66(9):1519-1522.

[6] Anvarinejad, M. ; Farshad, S. ; Alborzi, A. ; Ranjbar, R. ;Giammanco, G. ; and Japoni, A.(2011).Integron and genotype patterns of Quinolones- resistant uropathogenic Escherichia coli ., Afr. J.Microbial. Res Italy. 5 (22) : 3765-3770.

[7]Winn, J. W.; Allen, S.; Janda, W.; Koneman, E.; Procop, G.; Schreckenberger, P. and Woods, G. (2006). Koneman’s Color Atlas and Textbook of Diagnostic Microbiology, 6th ed., Lippincott–raven Publishers. Philadelphia, PP: 239–270. USA.

[8] Brown, A . (2007). Bensons Microbiological application laboratory manual in general microbiology . McGraw –Hill Co.INC. USA . P:102 -263.

[9] MacFaddin, J. F. (2000). Biochemical tests for identification of medical bacteria. 3rd ed. Lippincott Williams and Wilkins, USA.
[10] Collee, J. G.; Fraser, A. G.; Marmiom, B. P. and Simmon, A. (1996). Mackie and McCarteny, Practical Medical Microbiology. 4th ed. Churchill Livingstone inc.; USA.

[11] Bauer, A. W.; Kirby, W. M. M.; Sheris, J. C.; and Truck, M.(1966). Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol., 45: 493 – 496.
[12] Clinical and Laboratory Standards Institute (CLSI). (2012). Performance standards for antimicrobial susceptibility testing; 22ed. Informational Supplement. 32(3).

[13] Rossolini, G.M. and Mantengoli, E. (2005). Treatment and control of severe infections caused by multiresistant Pseudomonas aeruginosa. Clin. Microbiol Infect., 11:17 32

[14] Abdul-Wahid, A.A.(2014). Dissemination of Aminoglycosides Resistance in Pseudomonas aeruginosa Isolates in Al-Nasseryia Hospitals. M.Sc. Thesis. College of Medicine. University of Kufa.

[15] Aziz, Z. S. (2015). Study of carbapenems phenotypic resistance in Pseudomonas aeruginosa isolated from burns in Misan province. Journal of International Academic Research for Multidisciplinary.3(3): 2320-5083.

[16] Upadhaya, S. ; Shenoy, R. ; Shetty, V. ; Lamsal, A. ; Lamichhane, P. ; and Pokhrel, S. (2014). Multi-drug Resistant Pseudomonas aeruginosa Isolated from Intensive Care Burn Unit. International Journal of Biomedical Research, 5 (4) : 0976-9633.

[17] Avains , A.B. (2009). Identification of unusal pathogenic gram-negative aerobic and an aerobic . Afr. J. Med . Sci,24:135-139.

[18] Jane , M. (2007) . Clinical investigation the prevalence and resistance patterns of Pseudomonas aeruginosa in intensive care units in au niversity hospital . Turk . J . Med . Sci . 35 : 317-322.

[19] Al-Jubori,S.S.; Al-Jabiri, H.A.; and Al-Kadmy, I. M.S. (2015). Molecular Detection of Aminoglycoside Resistance Mediated by Efflux Pump and Modifying Enzymes in Pseudomonas aeruginosa Isolated From Iraqi Hospitals.. Int'l Conf. on Medical Genetics, Cellular & Molecular Biology, Pharmaceutical & Food Sciences.

[20] Al-Shara, J.M.R. (2013). Phenotypic and molecular detecting of carbapenem resistant Pseudomonas aeruginosa in Najaf Hospitals. Ph.D. Thesis. Faculty of Science. University of Kufa. Iraq.

[21] Al-Muhannak, F.H. (2010). Spread of some extended-spectrum beta-lactamases in clinical isolates of Gram-negative bacilli in Najaf. M.Sc. Thesis.College of Medicine.University of Kufa.

[22] Iwalokun, B.A.; Akinsinde , K.A.; Lanlenhin, O. and Onubogu, C. (2006). Bacterio cinogenicity and production of pyocins from Pseudomonas aeruginosa species isolated in Lagos, Nigeria. Afr. J. Biotechnology. 5(11) : 1072-1077.

[23] Al-Shalchi, S.A. ; Jubrael, J.M. and Krekor, A. (2001) . The use of RAPD-PCR atyping method for epidemiological inrestigation of Pseudomonas aeruginosa . The second conferencein Medical and Biological Sciences , 157-158.

[24] World Health Organization,WHO. (2002) .Prevention of hospital-acquired infections. Geneva, Switzerland . P.72.

[25] Fergie, J.E. ; Shama, S.J. ; Lott, L. ;Crawford, R. ; and Patrick, C.C.P. (1994). Pseudomonas aeruginosa bacteraemia in immunocomporomised children : analysis of factors associated with apoor outcome . Clin. Infect. Dis., 18: 390-394.

[26] Fayroz-Ali, J.M.H. (2012). Detection of quinolone resistance genes in Escherichia coli isolated from patients with significant bacteriuria in Najaf Province. Ph.D. Thesis. Sc.University of Babylon. Iraq.

[27] Al-Kabei, M.N.H.(2009). Isolation and Characterization of Lytic Bacteriophages Infecting Pseudomonas aeruginosa . M.Sc. Thesis. College of Sciences, Al-Qadisiya University.

[28] Raja, N.S.and Singh, N.N. (2007). Antimicrobial susceptibility pattern of clinical isolates of Pseudomonas aeruginosa in a tertiary care hospital. J. Microbiol.Immunol. Infect.,40:45-49.

[29] Al-Fatlawi, A.F.(2012). Detection of some Extended Spectrum Beta Lactamases Genes in Escherchia coli and Klebsiella spp. Isolated from Colon and Bladder Cancer Patiants in Al-Diwania City . M.Sc. Thesis. College of Medicine, Al-Qadisiya University.

[30] Gaynes, R. and Edwards, J. R. (2005). Overview of nosocomial infections caused by Gram-negative bacilli. Clin. Infect. Dis., 41:848-854 .

[31] Mahmoud, A. B.; Zahran, W. A.; Hindawi, G. R.; Labib, A. Z.; and Galal, R. (2013). Prevalence of multidrug-resistant Pseudomonas aeruginosa in patients with nosocomial infections at a University Hospital in Egypt, with special reference to typing methods. Journal of Virology & Microbiology : 13.

[32] Naqvi, Z.A.; Hashmi, K.; Rizwan, Q. and Kharal, S.A. (2005). Multidrug resistant Pseudomonas aeruginosa: A nosocomial insection treat in burn patients. Pakistan Journal of Pharmacology., 22(2):9-15 .
[33] Varaiya A, Kulkarni K, Kulkarni M, Bhalekar P, & Dogra J.( 2007) Incidence of metallo beta lactamase producing Pseudomonas aeruginosa in ICU patients. Department of Microbiology, S.L.Raheja Hospital, Mumbai, India.

[34] Sivaraj, S. ; Murugesan, P. ; Muthuvelu, S. ; Purusothaman, S. and Silambarasan, A.(2012). Comparative study of Pseudomonas aeruginosa isolate recovered from clinical and environmental samples against, 4 (3): 975-1491.

[35]Haldorsen, B.C. (2011).Aminoglycoside resistance in clinical Gram-negative isolates from Norway.M.Sc. thesis in medical biology. Norway. University of Tromso.

[36] Kim, J.Y.; Park, Y.J.; Kwon, H.J.; Han, K.; Kang, M.W. and Woo, G.J. (2008). Occurrence and mechanisms of amikacin resistance and its association with b-lactamases in Pseudomonas aeruginosa: a Korean nationwide study. J Antimicrob Chemother. 62:47983,doi:10.1093.

[37] Raja, N.S.and Singh, N.N. (2007). Antimicrobial susceptibility pattern of clinical isolates of Pseudomonas aeruginosa in a tertiary care hospital. J. Microbiol.Immunol. Infect.,40:45-49.

[38] Aghazadeh, M.; Hojabri, Z.; Mahdian, R.; Nahaei, M.R.; Rahmati, M.; Hojabri, T.; Pirzadeh, T. and Pajand, O. (2014). Role of efflux pumps: MexAB-OprM and MexXY (-OprA), AmpC cephalosporinase and OprD porin in non-metallo-β-lactamase producing Pseudomonas aeruginosa isolated from cystic fibrosis and burn patients. Infection, Genetics and Evolution. 24:187-192

[39] Magnet, S. and Blanchard, J.S. (2005). Molecular insights into aminoglycoside action and resistance. Chem. Rev., 105(2):477–498.

[40] Poulikakos, P. and Falagas, M.E. (2013). Aminoglycoside therapy in infectious diseases. Expert Opinion on Pharmacotherapy., 14(12):1585-1597 .

[41] DeMiguel Martinez, I.;Del Rosario Quintana, C.; Bolanos, Rivero, M. and Ramos Macias, A. (2005). Aetiology and therapeutic considerations in chronic otitis media. Analysis of a 5 years period. Acta. Otorrinolaringol. Esp. 56 (10): 459 – 462.

[42] Martinez, J. L. and Baquero, F. (2002). Interactions among strategies associated with bacterial infections: Pathogenicity, epidemicity and antibiotic resistance. C. M. R. 15 (4): 647 – 679.

[43] Sheng, W.H.; Chen, Y.C.; Wang, J.T.; Chang, S.C.; Luh, K.T. and Hsieh, W.C. (2002). Emerging fluoroquinolone-resistance for common clinically important gram negative bacteria in Taiwan. Diagn. Microbiol. Infect. Dis., 43:141–147.
How to Cite
ALRAMAHY, Syoof Khowman; FALH ALADILY, Zainab. Dissemination of some Antibiotic Resistance in Pseudomonas aeruginosa Isolates in Al-Diwaniya Hospitals.. Al-Qadisiyah Journal Of Pure Science, [S.l.], v. 22, n. 3, p. 349 - 359, feb. 2018. ISSN 2411-3514. Available at: <http://qu.edu.iq/journalsc/index.php/JOPS/article/view/613>. Date accessed: 17 dec. 2018.