Detection of fimA & fimC genes of Salmonella Typhimurium isolated from different sources

  • Abbas mayar hazam Collage of science- Al-Qadisiya university
  • Maitham Ghaly Yousif Collage of science- Al-Qadisiya university

Abstract

The collection of 500 different sample for the purpose of investigating the bacterium Salmonella typhimurium as included 140 stool specimens from children, male and female who admitted to the Teaching  Hospital  of  Maternity and Pediatrics,  in Al-Diwaniya ,90 a sample of stool pigeons ,142 a sample of the liver freezer and 128 sample of frozen meat examined in the laboratory of public health in Diwaniya, a laboratory of the Central Health in Baghdad.. The results of culturing on selective media, in addition to API 20 E system revealed that the rate of Salmonella isolates was (54/500)10.8% .  results of serotyping Salmonella isolates by using  monovalent antisera  revealed that 50 out of 54 isolates (92.6 %) belong to  S .typhimurium. Polymerase chain reaction (PCR) was used to detect  fimA gene encoding for biosynthesis of fimbriae A of S. typhimurium  and  fimC gene encoding for fimbriae C of  S. typhimurium,  PCR technique revealed that  5092.6%) ) isolates of S. typhimurium. were contain fimA  and fimC  gene.

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Published
2018-02-06
How to Cite
MAYAR HAZAM, Abbas; GHALY YOUSIF, Maitham. Detection of fimA & fimC genes of Salmonella Typhimurium isolated from different sources. Al-Qadisiyah Journal Of Pure Science, [S.l.], v. 22, n. 3, p. 444 - 453, feb. 2018. ISSN 2411-3514. Available at: <http://qu.edu.iq/journalsc/index.php/JOPS/article/view/614>. Date accessed: 12 dec. 2018.
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Articles