Evaluation of the Efficiency of Penicillium marneffei Segretain in control of larval instars of the mosquito Culex quinquefasciatus Say (Diptera: culicidae)
Keywords:
Culex quinquefasciatus, Say,, Wuchereria bancrofti, Peincillium marneffei, Bioassay
Abstract
The current research evaluate the efficacy of the fungus P. marneffei and using it as biological agent in the control of larval instars of the mosquito Cx. quinquefasciatus. Different concentrations of fungus suspention and its secondary metabolism were used in larvae of of mosquito, the result showed that the higher mortality percentage of first larval instars was 77.70% at the concentration of 2×10 4spore / ml after 120 hours, while the lowest mortality percentage was 59.5% at the lowest concentration of 2 × 10 2 spore / ml at the same period. But the application secondary metabolism showed high mortality percentage when 100% concentration was used against the mentioned instar and it reached 78.93% after 72 hours .while alower mortality percentage was recorded 55.77% concentration of 25% of secondary metabolism was used at the same period.Downloads
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References
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24. Mohanty,S.S. and Prakash,S. (2010). Comparative efficacy and pathogenicity of keratinophilic soil fungi against Culex quinquefasciatus larvae. Indian J Microbiol .50:299–302.
25. Nadeau , M.P. and Boisvert , J.I. 1994 . Larvicidal activity of the entomopathogenic fungus Tolypocladium cylindrosporum ( Deuteromycotina:Hyphoophomycetes ) on the mosquito Aedes triseviatus and the black fly Simulim vittatum ( Diptera : Simulidae ) . J. Am. Mosq. Control Assoc . 10 : 487 – 491 .
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29. Rajesh ,K.; Dhanasekaran,D. and Tyagi,K. (2014).Mosquito survey and larvicidal activity of actinobacterial isolates against Culex larvae (Diptera: Culicidae), Journal of the Saudi Society of Agricultural Sciences. 2 (6): 233-239 pp
30. Scholete , E.J. ; Taken , W. and Knols , B.G.J.( 2003) . pathogencity of five East African entomopathogenic Fungi to adult Anopheles gambiae ( Diptera : Culicidae ) mosquitoes , Netherlands Entomol. society 14 : 25 –29.
31. Singh, G and Prakash, S. 2010. Fungi Beauveria bassiana ( Balsamo) metabolites for controlling malaria and filarial in tropical countries. Advan. in Biomed. Res.9: 238-242.
32. Soni , N. and Prakash, S.E.( 2013). Possible Mosquito Control by Silver Nanoparticles Synthesized by Soil Fungus (Aspergillus niger 2587). Advances in Nanoparticles.2: 125-132pp.
33. Soni ,N. and Prakash,S. (2012). Larvicidal effect of Verticillium lecanii metabolites on Culex quinquefasciatus and Aedes aegypti larvae. Asian Pacific Journal of Tropical Disease . 2(3):220-224.
34. Soni ,N. and Prakash,S.(2011). Aspergillus Niger Metabolites Efficacies Against the Mosquito Larval (Culex Quinquefasciatus, Anopheles Stephensi and Aedes Aegypti) Population after Column Chromatography. American Journal of Microbiology . (1): 15-20.
35. Steinhause,E.A.(1949).Principle 0f in Pathology .NewYork.Tornto.London .Mc Graw-Hill book company INC.757.
36. Weiser , J. and Matha , V. (1988) . Tolypin , anew insecticidal metabolite of fungi of the genus Tolypocladium. J. of the invertebrate Pathol. 51 : 94 – 96.
37. WorldHealthOrganization.2013b.Lymphaticfilarias.http://www.who.int/mediacentre/factsheets/fs102/e/
2. عبد القادر ØŒ أياد عبد الوهاب . 2000 . دراسة تصنيÙية لعائلة البعوض (Diptera : Culicidae) ÙÙŠ Ù…ØاÙظة البصرة . اطروØØ© دكتوراه . كلية العلوم/ جامعة البصرة . 230 صÙØØ©.
3. الكرعاوي ØŒ هناء رØمن Ù„Ùته . 2012 . دراسة مختبرية لكÙاءة بعض طرائق السيطرة ÙÙŠ نوعين من البعوض (Diptera : Culicidae) ÙÙŠ Ù…ØاÙظة الديوانية . رسالة ماجستير . كلية العلوم / جامعة القادسية 94 صÙØØ©.
4. المØنة ØŒ اØمد غانم نوري . 2011 . تقييم ÙƒÙاءة الÙطر Metarhizium
anisopliae(Metschnikoff) Sorokin ÙÙŠ مكاÙØØ© نوعين من البعوض (Diptera : Culicidae ) ÙÙŠ Ù…ØاÙظة الديوانية . رسالة ماجستير . كلية العلوم / جامعة القادسية 69 صÙØØ© .
5. المشكور ,براء جليل سعيد .2014 . تقويم ÙƒÙاءة بعض عوامل المكاÙØØ© الجرثومية ÙÙŠ السيطرة على البعوض Culex quinquefasciatus (Diptera:culicidae) ,رسالة ماجستير .كلية العلوم /جامعة القادسية .64 صÙØØ©.
6. الموسوي ,منى ابراهيم جاسم .2015 . إنتاج وتوصي٠أنزيم البروتييز القاعدي من الÙطرMetarhizium anisopliae كعامل من عوامل السيطرة الØيوية تجاه عثة الشمع الكبرى Galleria mellonella,اطروØØ© دكتوراة كلية التربية للعلوم الصرÙØ© /جامعة كربلاء .201 صÙØØ©
7. Abbot , W. 1925 . A method of computing the effectiveness of insecticide . J. Econ. Entomol. 18 : 265 – 267
8. Abul-hab , J.K. 1968 . Larvae of culicine mosquitoes of Iraq with akey for their Identification . Bull. End – Dis . Baghdad . X(1 – 4) : 23 .
9. Ashoor, A. and Abu-Baleer, Y. (2002) . Is the Classical Classification of aspergillosis paranasal sinuses to non-invasive and Invasive still valid or not? Bahrain Medical Bull. 24: 91-94.
10. Balachander, M.;Remadevi, O.K.; Sasidharan, T.O and Bai, N.S.(2012). Virulence and mycotoxic effects of Metarhizium anisopliae on Mahogany shoot borer, Hypsipyla robusta (Lepidoptera:pyralidea). Journal of Forestry Research.23(4):651-659.
11. Becker ,N.; Petric ,D.; Boase, C.; Madon, M.; Dahl ,C. and Kaiser,A. (2010). Mosquitoes and Their Control, Springer Heidelberg Dordrecht London. New York. Library of Congress Control.Second Edition.608pp.
12. Benserradj ,O. and Mihoubi ,I.(2014) Larvicidal activity of entomopathogenic fungi Metarhizium anisopliae against mosquito larvae in Algeria . International Journal of Current Microbiology and applied Sciences . 3(1): 54-62
13. CDC. (June 2007). CDC answers your questions about St.Louis encephalitis. Center for Disease Control. http://www.cdc.gov/sle/technical/fact.html (2 June 2009).
14. Gottel , M.S. and Ingilis , D. 1997 . Fungi : Hyphomycetes . In lacey , L. (ed) Manual of techniques in insect pathology . Acadmic press . Sandiego . 409 pp .
15. Govindarajan, M.; Jebanesan, A.and Reetha, D. (2005) .Larvicidal effect of extracellular secondary metabolites of different fungi against the mosquito, Culex quinquefasciatus Say. Trop Biomed .22(1):1–3
16. Grove , J.F. and Pople , M. 1980 . The insecticidal activity of Beauveria and enniation complex . Mycopathology , 70 : 103 – 105.
17. James ,J. C.(2006). Understanding the Pathogenic Fungus Penicillium marneffei: A Computational Genomics Perspective.PHD thesis. University of Hong Kong.290pp.
18. Khan,S.;Guo,L.;Maimaiti,Y.;Mijit,M.;andQiu,D.(2012).Entomopathogenic Fungi as Microbial Biocontrol Agent. Molecular Plant Breeding .3)7:(63-79.
19. Lau, S.K.; Chow, W.N.; Wong, A.Y.; Yeung, J.M.; Bao, J.; Zhang, N.; Lok ,S. ;Woo, P.C. and Yuen, K.Y. (2013).Identification of microRNA-likeRNAs in mycelial and yeast phases of the thermal dimorphicfungus Penicillium marneffei. PLoS Negl Trop Dis.7(8): 2398-2410pp.
20. Lacey , L.A. 1997 . Manual of techniques in insect pathology ( Biological Techniques ) . Acadmic press . Sandiego – London – Boston – 408 pp.
21. Mohanty, S.S.and Prakash, S. (2000). Laboratory evaluation of Trichophyton ajelloi a fungal pathogen of Anopheles stephensi and Culex quinquefasciatus. J Am Mosq Control Assoc .16:254–257pp.
22. Mohanty, S.S.and Prakash, S. (2002). Efficacy of Chrysosporium lobatum against larvae of malaria vector, Anopheles stephensi in the laboratory. curr Sci. 83:1585–1588pp.
23. Mohanty, S.S.and Prakash, S. (2004). Extracellular metabolites of Trichophyton ajelloi against Anopheles stephensi and Culex quinquefasciatus larvae. Curr Sci. 86(2):323–325pp.
24. Mohanty,S.S. and Prakash,S. (2010). Comparative efficacy and pathogenicity of keratinophilic soil fungi against Culex quinquefasciatus larvae. Indian J Microbiol .50:299–302.
25. Nadeau , M.P. and Boisvert , J.I. 1994 . Larvicidal activity of the entomopathogenic fungus Tolypocladium cylindrosporum ( Deuteromycotina:Hyphoophomycetes ) on the mosquito Aedes triseviatus and the black fly Simulim vittatum ( Diptera : Simulidae ) . J. Am. Mosq. Control Assoc . 10 : 487 – 491 .
26. Nisbet, L.J., and Fox, F.M. (1991).The Biodiversity of Microorganisms and Invertebrates: Its Role In Sustainable Agriculture. London: CAB International.p. 445-650.
27. Pelizza , S.A. , Lopezlastra , C.C. and Becnel , J.J. 2008 . Research on the production , Longevity and Infectivity of the zoospores of Leptolegnia chapmanii Seymor (Oomycota : Peronosporomycetes ) . J. Invertbr. Pathol ., 98 : 314 – 319 .
28. Prakash, S.; Singh, G.; Soni, N.and Sharma, S.(2010). Pathogenicity of Fusarium oxysporum against the larvae of Culex quinquefasciatus (Say) and Anopheles stephensi (Liston) in laboratory. Parasitol Res . 107(3): 651-655.
29. Rajesh ,K.; Dhanasekaran,D. and Tyagi,K. (2014).Mosquito survey and larvicidal activity of actinobacterial isolates against Culex larvae (Diptera: Culicidae), Journal of the Saudi Society of Agricultural Sciences. 2 (6): 233-239 pp
30. Scholete , E.J. ; Taken , W. and Knols , B.G.J.( 2003) . pathogencity of five East African entomopathogenic Fungi to adult Anopheles gambiae ( Diptera : Culicidae ) mosquitoes , Netherlands Entomol. society 14 : 25 –29.
31. Singh, G and Prakash, S. 2010. Fungi Beauveria bassiana ( Balsamo) metabolites for controlling malaria and filarial in tropical countries. Advan. in Biomed. Res.9: 238-242.
32. Soni , N. and Prakash, S.E.( 2013). Possible Mosquito Control by Silver Nanoparticles Synthesized by Soil Fungus (Aspergillus niger 2587). Advances in Nanoparticles.2: 125-132pp.
33. Soni ,N. and Prakash,S. (2012). Larvicidal effect of Verticillium lecanii metabolites on Culex quinquefasciatus and Aedes aegypti larvae. Asian Pacific Journal of Tropical Disease . 2(3):220-224.
34. Soni ,N. and Prakash,S.(2011). Aspergillus Niger Metabolites Efficacies Against the Mosquito Larval (Culex Quinquefasciatus, Anopheles Stephensi and Aedes Aegypti) Population after Column Chromatography. American Journal of Microbiology . (1): 15-20.
35. Steinhause,E.A.(1949).Principle 0f in Pathology .NewYork.Tornto.London .Mc Graw-Hill book company INC.757.
36. Weiser , J. and Matha , V. (1988) . Tolypin , anew insecticidal metabolite of fungi of the genus Tolypocladium. J. of the invertebrate Pathol. 51 : 94 – 96.
37. WorldHealthOrganization.2013b.Lymphaticfilarias.http://www.who.int/mediacentre/factsheets/fs102/e/
Published
2018-02-06
How to Cite
AbduL-Hadi Alganimi, A., & R. Annon Alhasnawy, M. (2018). Evaluation of the Efficiency of Penicillium marneffei Segretain in control of larval instars of the mosquito Culex quinquefasciatus Say (Diptera: culicidae). Al-Qadisiyah Journal of Pure Science, 22(3), 431 - 443. Retrieved from https://journalsc.qu.edu.iq/index.php/JOPS/article/view/615
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