Effect of Vitamin E on in Vitro Sperm Activation of Asthenospermic Infertile Patients by using Swim-up technique

  • Dhirgam F.Al-Shimerty Pharmacy college\ University of Kufa
  • Sabah N.Alwachi Scince college\ University of Baghdad
  • YahiaK Al-sultani Pharmacy college\ University of Kufa


This Study was performed on 50 seminal fluid samples belongs to 50 patients suffering from Asthenospermia The objective of the persent study is to activation patient sperms quality in vitro by using swim-up technique for 30 minute of incubation in Earl’s medium  and Earl’s medium + vitamin E to find out the effect of vitamin E on the sperms parameter.                                                                  
     The result of sperm patients suffering from Ashenospermia  by using the swim-up technique (Earl’s media and Earl’s media + vitamin E)  showed that there was a significant decrease (P<0.05) in sperm concentration and leukocyte concentration in comparison to that used before activation.                       
     The sperm activation of patients suffering from Asthenospermia by using the swim-up technique (Earl’s media and Earl’s media + vitamin E) showed that there was a significant improvement (P<0.05) in the sperm motility percent, normal sperm morphology percent and sperm grade activity in comparison to its result befor activation Also, a significant improvement was noticed(P<0.05) in the sperm motility percent, sperm grade activity by using swim-up technique when Earl’s media was mixed with vitamin E in comparison to the process of activation in swim-up technique when only Earl’s media was used. However, there was a significant improvement (P<0.05) in sperm concentration swim-up technique by using Earl’s media and vitamin E  when compared to that in which only Earl’s media was used.                                                                                                             
     It was concluded that using a human sperm activation it was found that the addition of vitamin E to Earl’s media improve the sperm parameters and their activation in infertile patients suffering from Asthenospermia.


1-Cooper ,T.G.;Noonan.E and Von Eckandstein S.(2010). World Health organization reference values for human semen characteristics. Hum.Reprod.Uptake 16(3):231-45.
2-Visser,L. and Repping,S.(2010).Unraveling the genetics of spermatogenetic failure Reproduction;139:303-307.
3-Dohle ,G,R; Jungwirth,A; Copli , G; Giwercman, A; Diemer ,T and Hargreare , T,B(2007). Guidelines on male infertility .European association of Urology.P 6.
4-World, Health Organization (2010) . Laboratory Manual for the semen and sperm cervical Mucus Interaction, 1th ed, Cambridge University press, Cambridge.
5-Agarwal, A.; Saleh, R..A. and Bedaiwy, M..A. (2003) . Role of reactive oxygen species in the pathophysiology of Human Reproduction .Fertil.Steril; 79 : 829-843.
6-Pasqualotto, F.F.; Sharma, R.K.; Nelson, D.R.; thomas, A.J. and Agarwal, A. (2000) .Relationship between oxidative stress, semen characteristics, and Clinical diagnosis in men undergoing infertility investigation.Fertil.Steril; 73 : 459-464.
7-Agarwal, A. (2004) . Increased seminal reactive oxygen species levels in patients with varicoceles correlate with varicocele grade but not with testic size. Fertil.Steril; 82 : 1684-1686.
8-Bolle, P.; Fyandri, M.G. and Saso, I. (2002) .The controversial effeacy of vitamin E for human maic infertility.Contraception ; 65 : 313-315.
9-Sepalla, M. (1985) .The world Collaborative report on in vitro fertilization and embryo replacement. Current state of the art in January, 1984, Ann. Ny. Acad. Sci; 442 : 558-568.
10-الراوي ، خاشع محمود (2000) . مدخل الى الأحصاء ، الطبعة الثانية . كلية الزراعة والغابات , جامعة الموصل.
11-الهادي ، فارس ناجي عبود (1997) . استخدام التقنية الطبقية المزدوجة الترسيبية في تنشيط نطف مرضى العقم المصابين بقلة ووهن النطف . اطروحة دكتوراه ، كلية العلوم ، جامعة بغداد .
12-الشمرتي ، ضرغام فالح حسن (2008) . تأثير فيتامين E في تنشيط معالم النطف في الزجاج لمرضى العقم المصابين بقلة النطف ووهنها . أطروحة ماجستير ، كلية العلوم ، جامعة بغداد.
13-Stovall, D.W.; Guzick, D.S.; Berga, S.L.; Krasnow, J.S. and Zeleznik, A. J. (1994) . Sperm recovary and survival : Two tests that predict in vitro fertilization out come. Fertil .Steril; 62 : 1244-1249.
14-Menkveld, R.; Swanson, R.J.; Kotze, T.J.W. and Kruger, T.F. (1990). Comparsion of afiscontinuouspercoll gradient method versus aswim-up method : Effects on sperm morphology and other semen parameters. Andrologia; 22 : 152-158.
-15السلطاني ، يحيى كاظم (1997) . تنشيط النطف خارج الجسم لمرضى العقم والمصابين بقلة وابيضاض المني باستخدام المستنبتات الزراعية والهرمونات المحرضة للقند . اطروحة دكتوراه ، كلية العلوم ، جامعة بغداد .
16-Harris, S.J.; Milligan, M.P.; Masson, G.M.and Dennis, K.J. ( 1981) . Improved separation of motile sperm in asthenospermia and its application to artificial insemination homologous (AIH) .Fertil.Sertil; 36: 219-221.
17-Kasai, T.J.; Ogawa. K.; Mizuno, K.; Nagea, S.; Uchida, Y.; Ohta, S.; Fujie, M.; Suzuki, K.; Hirata, S.and Hoshi, K. (2002) . Relationship between sperm mitochondrial membrane Potential, Sperm motility and fertility Potential. Asian J Androl; 4 : 97-103.
18-Makler , A .(1998) . Modren methods in semen analysis evaluation .In : Progress in Infertility . Behrman, S.J; Kistner,RW. And Patton; G.W.(eds.),Little, Brown and Company, Boston,Toronto:633-661.
-19 المرشدي ، صاحب يحيى حسن (2006) . تأثير بيروكسيد الهيدروجين وبعض مضادات الأكسدة في معايير النطف البشرية خارج الجسم الحي . أطروحة دكتوراه ، كلية العلوم ، جامعة بابل.
20-Iwasaki, A. and Gagnon. C. (1992) .Formation of reactive oxygen species in spermatozoa of infertile patients.Fertil.Steril; 57 : 409.
21-Donnelly, E.T,; Lewis, S. E.M.; Mc-Nally,J.A. and Thompson, W.(1999) . Invitro fertilization and Pregnancy rates : The influence of sperm motility and morphology on IVF outcome. Fertile ; 70 : 305-314.
How to Cite
F.AL-SHIMERTY, Dhirgam; N.ALWACHI, Sabah; AL-SULTANI, YahiaK. Effect of Vitamin E on in Vitro Sperm Activation of Asthenospermic Infertile Patients by using Swim-up technique. Al-Qadisiyah Journal Of Pure Science, [S.l.], v. 22, n. 3, p. 406 - 414, feb. 2018. ISSN 2411-3514. Available at: <http://qu.edu.iq/journalsc/index.php/JOPS/article/view/616>. Date accessed: 16 dec. 2018.