Diagnosis of pseudomonas aeruginosa using PCR technique and determining their sensitivity to some antibiotics.
Keywords:
Pseudomonas aeruginosa, resistance, antibiotics, β-lactam antibiotics
Abstract
The study included the collection of pathological specimens from various sources by 360 sample of hospitals in AL- Diwaniyah city for the period of November 2015 to March 2016 and the samples divided, according to collected sources to (169 swab ear 0.102 swab burns, and 89 sample urine), results of cultural and biochemical tests showed that 50 isolate belong to Pseudomonas aeruginosa , tested the sensitivity of the isolates to 7 type of antibiotics, the study results indicated the presence of a relatively high resistance shown by P. aeruginosa to most antibiotics used in the study except Imipenem as Least resistance ratio among the rest of antibiotics 10%, as the study showed that there are 46 isolated (92%) are resistant to at least one of the used β-lactam antibiotics in this study that represent (Carbencilin, Piperacilin, cefotaxime, ceftriaxone, cefepime, Imipenem, and Azteronam ) that they were the highest percentage of Carbencilin (92%,74% ,88% ,82% ,80% ,10% ,68%) respectively.Downloads
Download data is not yet available.
References
1-Bojary, N.M.R. and M. Hajia,(2012).Multidrug- resistant Pseudomonas aeruginosa strains in Tehran Reference Burn Hospital, Tehran,Iran. African Journal of Microbiology Research,6: 1393-1396.
2- Brusselaers, N.; Vogelaers,D. and Blot,S.(2011). The rising problem of antimicrobial resistance in the intensive care unit. Annals Inten. C., 1:47.
3- Peirano, G.and Pitout, J.D.D.(2010).Molecular epidemiology of Escherichia coli producing CTX-M β-lactamases: the worldwide emergence of clone ST131 025:H4. International journal of Antimicrobial Agents, 35(4):316-321.
4- Winn, J. W.; Allen, S.; Janda, W.; Koneman, E.; Procop, G.; Schreckenberger, P. and Woods, G. (2006). Koneman’s Color Atlas and Textbook of Diagnostic Microbiology, 6th ed., Lippincott–raven Publishers. Philadelphia, PP: 239–270. USA.
5- Brown, A . (2007). Bensons Microbiological application laboratory manual in general microbiology . McGraw –Hill Co.INC. USA . P:102 -263.
6- MacFaddin, J. F. (2000). Biochemical tests for identification of medical bacteria. 3rd ed. Lippincott Williams and Wilkins, USA.
7- Collee, J. G.; Fraser, A. G.; Marmiom, B. P. and Simmon, A. (1996). Mackie and McCarteny, Practical Medical Microbiology. 4th ed. Churchill Livingstone inc.; USA.
8- Bauer, A. W.; Kirby, W. M. M.; Sheris, J. C.; and Truck, M.(1966). Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol., 45: 493 – 496.
9-Clinical and Laboratory Standards Institute (CLSI). (2012). Performance standards for antimicrobial susceptibility testing; 22ed. Informational Supplement. 32(3).
10-Selezska, K. and Ukraine, K. (2010). Pseudomonas aeruginosa population structure revisited under environmental focus. der Technischen Universität Carolo-Wilhelmina., 1-106.
11- سالم , رنا مشعل. (2014). انتشار انزيمات البيتالاكتاميز نوع OXA بين بكتيريا الزوائ٠الزنجارية والمعزولة من مشستشÙيات Ù…ØاÙظة الديوانية . رسالة ماجستير , كلية العلوم - جامعة القادسية.
12-داخل ,زينب ÙØ§Ù„Ø (2015). التØري عن بعض الجينات المقاومة للامينوكلايكوسيدات والكينولينات ÙÙŠ بكتريا Pseudomonas aeruginosa المعزولة من مصادر سريرية مختلÙØ© ÙÙŠ مدينة الديوانية. رسالة ماجستير ,كلية العلوم – جامعة القادسية.
13- Øران , عمر Øسين. (2012). التØري عن جينات المقاومة لمضادات البيتالاكتام من البكتيريا المعزولة من بعض الإصابات السريرية ÙÙŠ Ù…ØاÙظة الديوانية . رسالة ماجستير , كلية العلوم - جامعة القادسية .
14-Abdul-Wahid, A. A . (2014). Dissemination of Aminoglycosides Resistance in Pseudomonas aeruginosa Isolates in Al-Nasseryia Hospitals. M.Sc. Thesis. College of Medicine. University of Kufa .
15- Gad, G. F.; El-Domany, R. A.; Zaki, S. and Ashour,H. M. (2007). Characterization of Pseudomonas aeruginosa isolated from clinical and environmental samples in Minia, Egypt: prevalence, antibiogram and resistance mechanisms . Journal of Antimicrobial Chemotherapy., 60: 1010-1017.
16- Dohar, J. E. ; M. A. Kenna and R. M. Wadosky (1996). In Vitro Susceptibility of Actual Isolates of Pseudomonas aeruginosa to Commonly Used Ototopical Antibdies. American Journal Of Otology. 17 (2) : 207 – 209.
17- Goycoolea, M. and Ruah, L.(1991) .Definitions and Terminology .Otol Clin.of North Am ., -24:757761.
18- Pruitt, B.A.; McManus, A.T.; Kim, S.H.; and Goodwin, C.W. (1998).Burn wound infections: current status. World J Surg., 22: 135-145.
19- Magnet, MD. M. H.; Arongozeb, MD.; Khan,G. M.; and Ahmed, Z. (2013). Isolation and identification of different bacteria from different types of burn wound infection and study their antimicrobial sensitivity pattern. International Journal of Research in Applied, Natural and Social Sciences., 1(3) :125-132.
20- Gaynes, R. and Edwards, J. R. (2005). Overview of nosocomial infections caused by Gram-negative bacilli. Clin. Infect. Dis., 41:848-854.
21- Ibezim, E. C.(2005). Microbial resistance to antibiotics. African Journal of Biotechnology. In burn patients.Burns, 28(1).Isolates Causing 4(13): 1606-1611.
22- Anil, C. and Shahid, R. M. (2013). Antimicrobial susceptibility patterns of Pseudomonas aeruginosa clinical isolates at a tertiary care hospital in Kathmandu, Nepal. Asian J Pharm Clin Res., 6(3) : 235-238.
23- Livermore, D. M. and Brown, D. F. J. (2005). Detection of β-lactamase mediated resistance. Retrieved 31.
24- EL-Mosallamy, W. A., E.; Osman, A. S.; Tabl, H. A., E.; and AL-Tabbakh, A. M. (2015). Phenotypic and Genotypic Methods for Detection of Metallo-Beta-Lactamase (MβL) Producing Pseudomonas aeruginosa. Egyptian Journal of Medical Microbiology., 24(3) : 27-35.
25- Bush, K. 1988. -Lactamase inhibitors from laboratory to clinic. Clin. Microbiol. Rev. 1:109–123.
26- Du , S. J. ; Kuo, H. C. ; Cheng, C. H. ; Fei, A.C.Y. ; Wei, H. W. ; and Chang, S. K. (2010). Molecular mechanisms of ceftazidime resistance in Pseudomonas aeruginosa isolates from canine and human infections. Veterinarni Medicina, 55(4): 172-182.
27- Tirodimos, I.; Arvanitidou, M.; Dardavessis, T. ; Bisiklis, A.; and Alexiou-Daniil , S. (2010). Prevalence and antibiotic resistance of Pseudomonas aeruginosa isolated from swimming pools in northern Greece . Eastern Mediterranean Health Journal., 16 (7) : 783-787.
2- Brusselaers, N.; Vogelaers,D. and Blot,S.(2011). The rising problem of antimicrobial resistance in the intensive care unit. Annals Inten. C., 1:47.
3- Peirano, G.and Pitout, J.D.D.(2010).Molecular epidemiology of Escherichia coli producing CTX-M β-lactamases: the worldwide emergence of clone ST131 025:H4. International journal of Antimicrobial Agents, 35(4):316-321.
4- Winn, J. W.; Allen, S.; Janda, W.; Koneman, E.; Procop, G.; Schreckenberger, P. and Woods, G. (2006). Koneman’s Color Atlas and Textbook of Diagnostic Microbiology, 6th ed., Lippincott–raven Publishers. Philadelphia, PP: 239–270. USA.
5- Brown, A . (2007). Bensons Microbiological application laboratory manual in general microbiology . McGraw –Hill Co.INC. USA . P:102 -263.
6- MacFaddin, J. F. (2000). Biochemical tests for identification of medical bacteria. 3rd ed. Lippincott Williams and Wilkins, USA.
7- Collee, J. G.; Fraser, A. G.; Marmiom, B. P. and Simmon, A. (1996). Mackie and McCarteny, Practical Medical Microbiology. 4th ed. Churchill Livingstone inc.; USA.
8- Bauer, A. W.; Kirby, W. M. M.; Sheris, J. C.; and Truck, M.(1966). Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol., 45: 493 – 496.
9-Clinical and Laboratory Standards Institute (CLSI). (2012). Performance standards for antimicrobial susceptibility testing; 22ed. Informational Supplement. 32(3).
10-Selezska, K. and Ukraine, K. (2010). Pseudomonas aeruginosa population structure revisited under environmental focus. der Technischen Universität Carolo-Wilhelmina., 1-106.
11- سالم , رنا مشعل. (2014). انتشار انزيمات البيتالاكتاميز نوع OXA بين بكتيريا الزوائ٠الزنجارية والمعزولة من مشستشÙيات Ù…ØاÙظة الديوانية . رسالة ماجستير , كلية العلوم - جامعة القادسية.
12-داخل ,زينب ÙØ§Ù„Ø (2015). التØري عن بعض الجينات المقاومة للامينوكلايكوسيدات والكينولينات ÙÙŠ بكتريا Pseudomonas aeruginosa المعزولة من مصادر سريرية مختلÙØ© ÙÙŠ مدينة الديوانية. رسالة ماجستير ,كلية العلوم – جامعة القادسية.
13- Øران , عمر Øسين. (2012). التØري عن جينات المقاومة لمضادات البيتالاكتام من البكتيريا المعزولة من بعض الإصابات السريرية ÙÙŠ Ù…ØاÙظة الديوانية . رسالة ماجستير , كلية العلوم - جامعة القادسية .
14-Abdul-Wahid, A. A . (2014). Dissemination of Aminoglycosides Resistance in Pseudomonas aeruginosa Isolates in Al-Nasseryia Hospitals. M.Sc. Thesis. College of Medicine. University of Kufa .
15- Gad, G. F.; El-Domany, R. A.; Zaki, S. and Ashour,H. M. (2007). Characterization of Pseudomonas aeruginosa isolated from clinical and environmental samples in Minia, Egypt: prevalence, antibiogram and resistance mechanisms . Journal of Antimicrobial Chemotherapy., 60: 1010-1017.
16- Dohar, J. E. ; M. A. Kenna and R. M. Wadosky (1996). In Vitro Susceptibility of Actual Isolates of Pseudomonas aeruginosa to Commonly Used Ototopical Antibdies. American Journal Of Otology. 17 (2) : 207 – 209.
17- Goycoolea, M. and Ruah, L.(1991) .Definitions and Terminology .Otol Clin.of North Am ., -24:757761.
18- Pruitt, B.A.; McManus, A.T.; Kim, S.H.; and Goodwin, C.W. (1998).Burn wound infections: current status. World J Surg., 22: 135-145.
19- Magnet, MD. M. H.; Arongozeb, MD.; Khan,G. M.; and Ahmed, Z. (2013). Isolation and identification of different bacteria from different types of burn wound infection and study their antimicrobial sensitivity pattern. International Journal of Research in Applied, Natural and Social Sciences., 1(3) :125-132.
20- Gaynes, R. and Edwards, J. R. (2005). Overview of nosocomial infections caused by Gram-negative bacilli. Clin. Infect. Dis., 41:848-854.
21- Ibezim, E. C.(2005). Microbial resistance to antibiotics. African Journal of Biotechnology. In burn patients.Burns, 28(1).Isolates Causing 4(13): 1606-1611.
22- Anil, C. and Shahid, R. M. (2013). Antimicrobial susceptibility patterns of Pseudomonas aeruginosa clinical isolates at a tertiary care hospital in Kathmandu, Nepal. Asian J Pharm Clin Res., 6(3) : 235-238.
23- Livermore, D. M. and Brown, D. F. J. (2005). Detection of β-lactamase mediated resistance. Retrieved 31.
24- EL-Mosallamy, W. A., E.; Osman, A. S.; Tabl, H. A., E.; and AL-Tabbakh, A. M. (2015). Phenotypic and Genotypic Methods for Detection of Metallo-Beta-Lactamase (MβL) Producing Pseudomonas aeruginosa. Egyptian Journal of Medical Microbiology., 24(3) : 27-35.
25- Bush, K. 1988. -Lactamase inhibitors from laboratory to clinic. Clin. Microbiol. Rev. 1:109–123.
26- Du , S. J. ; Kuo, H. C. ; Cheng, C. H. ; Fei, A.C.Y. ; Wei, H. W. ; and Chang, S. K. (2010). Molecular mechanisms of ceftazidime resistance in Pseudomonas aeruginosa isolates from canine and human infections. Veterinarni Medicina, 55(4): 172-182.
27- Tirodimos, I.; Arvanitidou, M.; Dardavessis, T. ; Bisiklis, A.; and Alexiou-Daniil , S. (2010). Prevalence and antibiotic resistance of Pseudomonas aeruginosa isolated from swimming pools in northern Greece . Eastern Mediterranean Health Journal., 16 (7) : 783-787.
Published
2018-02-08
How to Cite
Hamoodi Jabbar, A., & Khowman. Alramahy, S. (2018). Diagnosis of pseudomonas aeruginosa using PCR technique and determining their sensitivity to some antibiotics. Al-Qadisiyah Journal of Pure Science, 22(3), 454 - 464. Retrieved from https://journalsc.qu.edu.iq/index.php/JOPS/article/view/622
Issue
Section
Articles
Copyright © Author(s) . This is an open access article distributed under the Creative Commons Attribution
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly
cited.
