Diagnosis of Proteus mirabilis using PCR technique and determining their sensitivity to some antibiotics.

  • Nuha Jawad Biology Department - College of Sciences - University of Al-Qadisiyah
  • Syoof Khowman. Alramahy Biology Department - College of Sciences - University of Al-Qadisiyah

Abstract

Proteus mirabilis The samples of the study were collected from different clinical sources 185 isolates of hospitals, the Diwaniyah city during the period from October 2015 to April and divided the samples, according to sources collected into five groups (46 swab ear, 42 swab burns, 37 a stool sample, 59  urine sample and 1 blood sample), as results showed that cultural and biochemical tests 69 isolated belong to P.mirabilis, diagnosis was confirmed by api 20E and the use of polymerase chain reaction.         Tested the sensitivity of isolates P.mirabilis about 15 types of antibiotics by disk diffusion method, and belonging to ten classes of antibiotics,That shown sensitivity first to isolates of P.mirabilis results, the proportion of resistant P.mirabils antimicrobial β- lactam antibiotic represented in Amoxicillin resistance and Ampicillin 84.05%, Amoxicillin/cluvanic acid 47.82%, Ticarcillin and , Cefatozidime 66.66%, Cefotaxim 53.62%, Meropenem 5.6%. The rate of resistance to antibiotics aminoglycosides represented in Amikacin resistance 17.36 %, Gentamicin percentage resistance 33.33%, Quinolones represented in Nalidic acid resistance 75.36%, while the Ciprofloxacin counted lower proportion of resistance 4.34 %, Trimethoprim resistance75.36 %.Chloramphenicol  have high     resistance rate of 82.60 %, as well as all of the Tetracycline 82.60%, and Rifampin  91.30% .  

References

1.Armbruster, C. E. and Mobley, H. L. 2012. Merging mythology and morphology: the multifaceted lifestyle of Proteus mirabilis. Natu. Revi. Microbio.. 10(11), pp: 743-754.
2-Jacobsen, S. M. & Shirtliff, M. E. (2011). Proteus mirabilis biofilmsand catheter-associated urinary tract infections. Virulence 2, 460–465.Iran. EMHJ, 18(2). 3. Pellegrino. R.; Scavone, P.; Umpiérrez, A.; Maskell, D.J.and Zunino, P.(2013). Proteus mirabilis uroepithelial cell adhesin (UCA) fimbria plays a role in the colonization of the urinary tract. J. Phatho. Dis., 2(67):104-107. 4.Mollenkopf ,D.(2012) .Epidemiology of CTX-M cephalosporinase – bearing Escherichia coli and Salmonalla spp isolated from US livestock .M.Sc .Thesis, college of science ,Ohio state University
5. Dhillon R, Clark J (2011). ESBL: A clear and present danger? Crit. Care Res. Pract. 2012 (2012):1-11
6.Winn, J. W.; Allen, S.; Janda, W.; Koneman, E.; Procop, G.; Schreckenberger, P. and Woods, G. (2006). Koneman’s Color Atlas and Textbook of Diagnostic Microbiology, 6th ed., Lippincott–raven Publishers. Philadelphia, PP: 239–270. USA.
7.CLSI (Clinical and Laboratory Standards Institute) (2012). Performance standards for Antimicrobial Susceptibility Testing; 23th Information Supplement 33(1). Wayne, Pannsylvannia, USA. 8. Thomas, L.C. (2007). Genetic methods for rapid detection of medicallyimportantnosocomial bacteria. Faculty of Medicine, Department of Medicine, The University of Sydney, Australia
9. Shoff, W. H. and Green-McKenzie, J. (2007). Pyelonephritis, acute.. J. Appl. Microbiol. 100: 1028–1033. 10. ALJumaa, M. H. (2011) . Bacteriological and Molecular Study of SomeIsolate of Proteus mirablis and Proteus vulgaris in Hilla Province.M.S.C. Microbiology.Thesis. College of Medicine.University. Iraq . 11.Abbas, K. F.; Al Khafaji,J. K. ; Al-Shukri , M. S. (2015). Molecular Detection of Some Virulence Genes in Proteus Mirabilis Isolated from Hillaprovince. International Journal of Research Studies in Biosciences (IJRSB). 3(10):PP85-89.
12.ALDulami,A.A;Nauman,N.G;andHasan,A.R.S(2011).Virulance Factors of Proteus mirablis Isolated From Patients Otitis Media in Baquba and it's Peripheries 1. Diyala. J. of Med. (69);(69-75).
13.Kwiecinska-Pirog , J ; Skowron ,K ; Bartczak ; and Gospodarek-Komkowska ,E .(2016). The Ciprofloxacin Impact on Biofilm Formation by Proteus Mirabilis and P. Vulgaris Strains . Jundishapur J Microbiol. 9(4):e32656.
14.Zuhir , R; Alaubydi, M. A. S. (2016). Extraction and Partial Purification of Lipopolysaccharide from Clinical Proteus mirabilis Isolate and Compared with Standard Bacteria. Iraq. Jo. of Sci,. 57, (1): 599-608.
15. Pal, N.; Sharma,N.; Sharma,R.; Hooja,S.; and K Maheshwari , R .(2014) . Prevalence of Multidrug (MDR) and Extensively Drug Resistant (XDR) Proteus species in a tertiary care hospital, India. Int.J.Curr.Microbiol.App.Sci 3(10) 243-252.
16.Senthamarai ,S. Sivasankari, S.; Anitha ,C.; Kumudavathi ,MS.; Amshavathani ,SK.; Venugopal ,V.; and Thenmozhi Valli, P R.(2015). A study on the antibiotic susceptibility pattern of Proteus spp among various samples. IJAPBC – Vol. 4(2): 2277 – 4688.
17.Wang, J; Chen ,P; Chang ,S ; Shiau, Y ; Wang, H ; Lai ,J ; Huang, I Tan ,M ; Lauderdale ,T,Y; and Hospitals ,T.( 2014). Antimicrobial susceptibilities of Proteus mirabilis: a longitudinal nationwide study from the Taiwan surveillance of antimicrobial resistance (TSAR) program .BMC Infec .Dis. 14:486.
18.Adnan,M.; Hussein,I.; and Al-DeresawiP3 ,A. M.S.(2014). Molecular detection of Proteus mirabilis using PCR technique among urinary tract infection patients. Ira. Jo. of Biote,.13(2 ): 35-47
19.Brooks , G.F. ; Carroll , K. C. ; Butel , J.S. and Morse , S. A. (2007) . Jawets, Melnick, Adelberg's Medical microbiology. 24th ed. McGraw-Hill com. PP. 174.
20. الطائي,خالد عبد الكاظم ,ناجي,حسن فاضل.(2013).تأثير خلط مضادات البيتالاكنم مع الامينو كلايكوسيدات على المتقلبات المتعددة المقاومة للمضادات الحيوية.مجلة جامعة بابل/ االعلوم الصرفة والتطبيقية . 4(21):( 1293-1299).
21.Freedman, A. L. (2005). Urologic Diseases in North America Project: trends in resource utilization for urinary tract infections in children. J. Urol., 173(3):949-954 22.A.Bahashwan,S.(2013).Antimicrobial resistance patterns of proteus isolates from clinical specimens. Eur. Sci. Jo, 9(27): 1857 – 7881
23. الحسو,محمود زكي,خلف,صبحي حسين.(2013). مقاومة بعض العصيات السالبة لصبغة كرام من اصابات الجهاز التنفسي السفلي لمضادات البيتالاكتم .مجلة علوم الرافدين ,24(6):(66-76).
24.Hussein ,A .A .(2013) . phenotypic detection of extended-spectrum betalactamase production in Proteus mirablis isolation from patients with Significant Bacteriuria in Najaf Provina .QMJ ,9(16);(146-160).
25.Perween, N; Prakash.S, K; and Bharara ,T.(2016). Prevalence of Multidrug-Resistant and Extensively Drug-Resistant Proteus, Providencia and Morganella Species in Burn Wound Infection. Inter. Jo of Scien.Study .Vol 3 (11).154-156. 26.Guilfoile, G.(2007). Deadly disease and epidemics Antibiotic-Resistant Bacteria. Chelser House publisher , World Health organization. P:10-119.
27.Hasanin, A; Eladawy, A; Mohamed ,H; Salah ,Y ; Lotfy ,A; Mostafa ,H; Ghaith ,D; Mukhtar, A. (2014). Prevalence of extensively drug-resistant gram negative bacilli in surgical intensive care in Egypt.Pan Afric. Medic. J. 19:177.4307.

28.Nahar , A. ; Siddiquee , M.; Nahar,S.; Anwar2,K . S.; Ali , S .k . Islam1, I.S.(2014). Multidrug Resistant-Proteus Mirabilis Isolated from Chicken Droppings in Commercial Poultry Farms: Bisecurity Concern and Emerging Public Health Threat in Bangladesh. J Biosafety Health Educ ,2(2): 2332-0893.
Published
2018-03-08
How to Cite
JAWAD, Nuha; KHOWMAN. ALRAMAHY, Syoof. Diagnosis of Proteus mirabilis using PCR technique and determining their sensitivity to some antibiotics.. Al-Qadisiyah Journal Of Pure Science, [S.l.], v. 22, n. 3, p. 692 - 703, mar. 2018. ISSN 2411-3514. Available at: <http://qu.edu.iq/journalsc/index.php/JOPS/article/view/633>. Date accessed: 17 dec. 2018.
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Articles