Prevalence of OXA beta -lactamaes of pseudomonas aeruginosa in Al-Diwaniya City

Abstract

                   In this study, 390 samples were collected from different sources from Al-Diwaniya teaching  Hospital during the period of November 2011 until March 2012,and they includ ( 292 clinical specimens and 98 environmental sample) , the results of cultural and biochemical tests showed that 50 isolates (39 of them were from clinical cases and 11 were from environmental samples),belonged to p. aeruginosa , and their diagnosis were confirmed by 16s-ribosomal RNA ,and the study showed that all the isolates containing the 16s-ribosomal RNA gene , which represents the designed diagnostic gene in this study .      The ability of these isolates to produce broad-spectrum OXA β- lactames enzymes groups was investigated thrrough detection of presence of genes blaOXA-10, blaLCR-1, blaOXA-18, blaOXA-1, blaOXA-2 in these isolates by using the polymerization chain reaction enzyme technology] (PCR) it showed 50/50 (100%) isolates contain blaOXA-10 gene, which belongs to the OXA group I, and the results of this study showed no amplification results for blaOXA-18, blaLCR-1, blaOXA-1, blaOXA-2 genes ,which belong to the main groups of broad-spectrum OXA β- lactames enzymes which are OXAgroup II ,OXAgroup III and OXA groupV respectively except OXA18 enzyme which is not belonging to any of these groups .