Molecular Detection of Virulence Factors Genes in Pseudomonas aeruginosa Isolated from Different Infections Cases in Al-Diwaniya Hospital

Abstract

Pseudomonas aeruginosa is the most important and dangerous organism in human infections due to production of several extracellular and cell-associated virulence factors that cause severe tissue damage, the most important virulence factors including (exoenzyme S, exotoxinA and lipoprotein), these factors was encoded by (exoS, toxA and oprI gene respectively). In this study we used polymerase chain traction technique (PCR) for detection virulence factor genes producing by P. aeruginosa that isolated from wound, burn and pulmonary tract infections patient swab samples. The PCR results was show that all P. aeruginosa isolates was carried virulence factors genes with difference in prevalence between them. The burn infection P. areuginosa isolates were show high prevalence of virulence factors genes more than wound and pulmonary tract infections isolates as well as the virulence factor gene (ToxA) was show high production in most isolates. In this study we concluded that the production of virulence factors genes in P. aeruginosa is important to human infection especially (ToxA) gene and the PCR technique is very specific and fast method in detection virulence factor genes in P. areuginosa.