Epidemiological study of the bacterium Proteus mirabilis isolated from clinical cases and investigate the molecular basis of resistance to antibiotics

Abstract

290   specimens were collected from different clinical cases of people who admitted and seen at Diwaniya Teaching Hospital.the specimens were distributed to 123 specimens originating male and 167 specimens source females. The total number of bacterial isolates has reached 278 isolates included: Pseudomonas aeruginosa, Pseudomonas fluorescence, Proteus mirabilis, Proteus vulgaris, Escherichia coli, Klebsella pneumonia, Staphylococcus sp. , Enterococcus sp. And Streptococcus sp., the number of bacterial isolates originating in females is the highest, amounting to 159 (57.58%) compared to males, which reached 119 (41.03%) .the bacteria P. mirabilis were 24 isolations  (8.27%) were distributed among the 10 (3.44%) and 14 (4.82%) for males and females respectively, too.   The sensitivity of bacterial isolates had been tested to six antibiotics which more common in health institutions. Sensitivity was varied according to antibiotic type and tested isolate  .The isolates of P. mirabilis showed high resistance to antibiotics trimethoprim, nitrufuration and tetracycline as it was (100%, 100%, 87.5% ) respectively . While given high sensitivity to amikacin and amoxicillin as it reached (95.84%).  Resistance genes by molecular detection PCR were identified nfsA , tetA , tri genes.these genes responsible for bacterial resistance to the recipe isolates of P. mirabilis bacteria to antibiotics Nitrofurantoin, Tetracycline, Trimethoprim. Some virulence factors of P. mirabilis isolates  were also detected and the results showed all isolates possessed phenomenon swarming, while that all isolates can not produce capsule , and some isolates produce Heamolycin (87.5%) and the percent  of isolates capable of producing protease 79.16%; As for the ability of the isolates adhesion, it may have had a high capacity above 80 cell on epithelial cell.