A Genetic Polymorphism of Interleukin 10 Gene at Position-1082 In Iraqis Patients with type 1 diabetes of

  • Ibtesam Badday Hassan University of Diyala. college of Education for pure science, Department of biology.
  • Azdihar Mohammed Jasem University of Diyala. College of Science, Department of Biology
  • Haneen Akram Habeeb Ministry of Education. Directorate of curricula and books, Biological Unit.

Abstract

The aim of this study was to evaluate the frequency of polymorphism of interleukin-10 Gene at position-1082 of the promoter region (IL-10-1082 SNP) in T1D and in healthy. The subjects were determined in 39 Iraqi patients, (12 males & 27 females; 1 5.65 ± 1.79 years) and 21 controls (7 male and 14 female; 14.66 ± 3.43 years) by polymerase chain reaction-specific sequence primer (PCR-SSP) assay, followed by electrophoresis on agarose-gel. The results revealed that comparing IL10 -1082 genotypes and alleles between T1D patients and controls it was observed that frequency of GA genotype was significantly higher (56.41vs. 42.85%; RR =23.7). may be considered as a risk factor for T1D While  A allele  was not significantly in patients contrast to controls, (41.03vs. 40.48%; RR =0.9)  and the associated EF values were 1.73and 1.02, respectively. In contrast, GG genotype and G allele (30.76vs. 58.97%, P =0.579 respectively) frequencies were significantly decreased in patients, compared to controls (38.09vs. 59.52%; P =1.000), and the associated PF values were 0.72 and 0.98, respectively . Similar observations were made in AA genotype. These findings suggest that IL10-1082 polymorphism showed associations (positive and negative) with T1D in the samples of Iraqi patients . Therefore,  the   functional   role  of  such  IL10-1082   might  have  been  altered  due  to  the  deviations  of  some  genotype  and  allele  frequencies.

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Published
2018-05-01
How to Cite
Badday Hassan, I., Mohammed Jasem, A., & Akram Habeeb, H. (2018). A Genetic Polymorphism of Interleukin 10 Gene at Position-1082 In Iraqis Patients with type 1 diabetes of. Al-Qadisiyah Journal of Pure Science, 23(1), 17 - 23. https://doi.org/10.29350/jops.2018.23.1.712
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Articles