Detecting of virulence factors COWP gene and CP15 gene for Cryptosporidium parvum by polymerase chain reaction (PCR)

Abstract

The   present   study aims to diagnose the parasite Cryptosporidium parvum isolated stool samples of patients to reviewers Diwaniya Teaching Hospital and Maternity  Hospital and Children in the province of Diwaniyia .The infected ranged from the age  less than one year to 12 years at the period from beginning of May to the end of November 2016.                                                                                                               .        110 samples stool were collected and the number of infected samples is (30) sample. After examining samples in direct examination of the sample wet method.                                                   The study included verification of virulence factors Cryptosporidium Oocyst Wall Protein (COWP), Surface Protein of Sporozoite (CP15) in parasite using conventional PCR technique.                                                                                                                                            The study showed that age groups less than one year were the highest infection rate (43.3%) while the age group of (9-12) years showed lowest infection rate (10%) with a significant difference between them, infection rate in females (56.6%) was higher than males (43.3%) but did not reach the level of significant.                                                                                                  These ratios also appeared in the rural areas are the highest (63.3%) compare with  urban areas (36.6%) with a significant difference between them.                                                                   DNA was extracted from positive stool samples and then after amplified using virulence factors of parasite COWP gene (281bp), CP15 gene (230bp) and the results appeared present these factors in all positive samples of C.parvum.