Cu\Zn SOD enzyme activity, partial purification from patients with diabetes type 1

Abstract

        Antioxidants are agents that prevent or reduce the level of oxidative damage to biomolecules in the living organisms. Antioxidants can be enzymatic or non-enzymatic. An enzymatic antioxidants such as catalase, superoxide dismutase (SOD), and glutathione peroxidase (GPx) predominantly have metal chelators. Cu/Zn SOD enzyme, reacts with superoxide radical to form H2O2, which will get converted to H2O. In this study, (Cu\Zn)SOD was isolated from human RBCs using DEAE-cellulose chromatography. The enzyme was purified  from  patients with diabetes type 1 and normal people as well .The (Cu\Zn)SOD activity was measured  in the serum of 20 samples of patients with diabetes  and 20 normal samples