Purification and Charactrization of protease for some plants sources

  • Rawaa majid dakhil AL-Sefar
  • Nazar abid Al- Amer Hamza

Abstract

The protease extract from Citrullus colocynthis L. was purified  by several  steps ,including precipitation with ammonium sulfate 60%, ion exchange chromatograghy using DEAE- Cellulose and gel filtration on Sepharose-6B column. The result showed purification fold and recovery (15.01) and (9.1)% respectively .Its molecular weight was estimated to be 50118kDa.The optimum pH for protease  was( 7.5 ) and stable in the pH range of (7-8.5) .The protease showed maximum activity at 35 C.using 1% as a substrate ,and  showed heat stability at (25-40) C . The results showed in crease in the activity with Ca++ and Na+ It was 112% and 121% respectively. The enzyme  strongly inhibited by PMSF but not by EDTA.The results indicate that the protease is aserine protease.

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Published
2018-05-24
How to Cite
majid dakhil AL-Sefar, R., & abid Al- Amer Hamza, N. (2018). Purification and Charactrization of protease for some plants sources. Al-Qadisiyah Journal of Pure Science, 22(4), 105 - 129. Retrieved from https://journalsc.qu.edu.iq/index.php/JOPS/article/view/780
Section
Articles