Gene expression profile of four immune genes in AIDS patients

Abstract

Human immunodeficiency virus (HIV) is one of Retroviruses which cause acquired immunodeficiency syndrome (AIDS) associated with severe effect on immune system.  Retroviruses possess a reverse transcriptase (RT) enzyme that convert viral RNA template into DNA which integrates with cellular DNA to cause persistent infection. The relationship between HIV and the immune system has also yielded important insights into mechanisms of immune homeostasis and host defenses in general. HIV causes impact on expression of immune genes. Four immune genes were subjected to this study using analysis of Bioinformatics tools represented by Real-time-reverse transcription PCR-array (RT-PCR-array) (SABioscience). Control genes were taken from previous studies to get the fold change of gene expression. The results showed that all subjected genes were up regulated with different fold change for each one (IFNA2, 11.2713 fold; IL10, 103.5786 fold; IL10RA2, 12.0803 fold; CXCL8, 34.1682 fold). These results may give important information to comprehend the relationship between HIV and human immune cells.