Immunohistochemical study for localization detection of insulin like growth factors(IGF1) in testiscular tissue of immature and mature male Wistar rats.

Abstract

The present study has been carried to investigate the immunological localization of Insulin-like Growth Factor 1 (IGF1) in pre- and post-pubertal stages and its involvement in male reproductive physiology of rats, Forty immature and mature male Wistar albino rats were used in the present study which assigned into 5 equal groups, 8 per each at 25th, 35th, 45th, 55th, and 65th day of age, male rats of each group have been anaesthetized. Testes tissue samples were obtained for immunohistochemical study. The results of immunohistochemical study showed the presence of IGF1 immunoreactivity in,sertoli cells, leydig cells and all cells that Constituent of seminiferous tubule, and showed density immunoreactivity increase during pubertal stage. Also observed the presence of IGF-1 immunoreactivity in mature sperm in seminiferous tubule lumen of post-pubertal testis.