Molecular detection of Escherchia coli carrying uropathogenic-specific-protein and typeâ…¢ secretion system from patients with urinary tract infection

  • Alaa A. Jawad Veterinary Medicine College, University of Al-Qadisiyah, Iraq
  • Hayder N. Ayyez Veterinary Medicine College, University of Al-Qadisiyah, Iraq
  • Miran Al-Rammahi Veterinary Medicine College, University of Al-Qadisiyah, Iraq
DOI: https://doi.org/10.29350/jops.2018.23.3.892

Abstract

To specify Escherichia coli, infects urinary bladder, whether it is a urospecific bacterium or not, this polymerase-chain-reaction (PCR)-based study was performed. Urine  specimens from patients with urinary tract infection  were subjected to a process of bacterial recovering 20 isolates of  E. coli. The isolates were then used for PCR-based identification of uropathogenic specific protein (usp) and type III secretion system ( ETTT) genes. The results revealed the presence of usp in 6 (30%) isolates and ETTT in 2 (10%) isolates.  So these results  give no-doubt confirmation that these isolates of E. coli are urospecific and pathogenic bacteria.

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Published
2018-09-03
How to Cite
A. Jawad, A., N. Ayyez, H., & Al-Rammahi, M. (2018). Molecular detection of Escherchia coli carrying uropathogenic-specific-protein and typeâ…¢ secretion system from patients with urinary tract infection. Al-Qadisiyah Journal of Pure Science, 23(3), 70 - 75. https://doi.org/10.29350/jops.2018.23.3.892
Issue
Vol 23 No 3 (2018)
Section
Articles

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