Molecular detection of Escherchia coli carrying uropathogenic-specific-protein and typeâ…¢ secretion system from patients with urinary tract infection

  • Alaa A. Jawad Veterinary Medicine College, University of Al-Qadisiyah, Iraq
  • Hayder N. Ayyez Veterinary Medicine College, University of Al-Qadisiyah, Iraq
  • Miran Al-Rammahi Veterinary Medicine College, University of Al-Qadisiyah, Iraq

Abstract

To specify Escherichia coli, infects urinary bladder, whether it is a urospecific bacterium or not, this polymerase-chain-reaction (PCR)-based study was performed. Urine  specimens from patients with urinary tract infection  were subjected to a process of bacterial recovering 20 isolates of  E. coli. The isolates were then used for PCR-based identification of uropathogenic specific protein (usp) and type III secretion system ( ETTT) genes. The results revealed the presence of usp in 6 (30%) isolates and ETTT in 2 (10%) isolates.  So these results  give no-doubt confirmation that these isolates of E. coli are urospecific and pathogenic bacteria.
Published
2018-09-03
Section
Articles