Serological investigation of Brucella spp. and Toxoplasma gondii in horses by using rose Bengal and toxoplasma latex tests in Dhi-Qar province, Iraq

  • Jalil Abed Gatie and Saleem Amin Hasso Veterinary Department, Ministry of Agriculture, Iraq and Department of Internal Veterinary Medicine, College of Veterinary Medicine, University of Baghdad, Iraq.


To detect the infection by Brucella spp. and Toxoplasma gondii. This study was conducted using a screening Rose Bengal and Toxoplasma latex tests on 100 blood samples collected from horses (18 male +82 female) average of age (1month-25 years) living in different areas in the districts of Dhi-Qar province in southern Iraq, where results showed a spread of Brucella spp. antibodies germs by 8% and the spread of the parasite Toxoplasma gondii antibodies 21%. This study showed the spread of infection by bacteria Brucella and Toxoplasma parasite in horses in Dhi-Qar. Province-southern Iraq.


1-Osman Y, TEL N, Arserim R, Oktay K. Seroprevalence of Equine Brucellosis in Southeast Turkey.YYU Veteriner Fakultesi Dergisi, (2011); 22 (3): 181-183.
2-Getachew T, Getachew G, Sintayehu G, Getenet M, Fasil A. Bayesian estimation of Sensitivity and specificity of Rose Bengal, complement fixation, and indirect ELISA tests for the diagnosis of bovine brucellosis in Ethiopia. Veterinary Medicine International., (2016) ;(3):753-755.
3-Al-Khafaji WS, Al-Talibi MAM Alsaad K, Hassan SD. Investigation for the presence of brucella antibodies in equine in Mosul city using rose Bengal and indirect elisa tests. Bas. j. Vet. Res., (2011); 10 (1):43-48.
4-Akca A, Babur C, Arslan MO, Gicik Y, Kara M, Kilic S. Prevalence of antibodies to Toxoplasma gondii in horses in the province of Kars, Turkey. Vet. Med.-Czech, (2004); 49(1): 9-13.
5-Jin-Lei W, Dong-Hui Z, Jia C, Guang-Xue L,Wen-Bing P, Ting-Yu L, Si-Yuan Q, Ming-Yang Y, Xing-Quan Z. The prevalence of antibodies to Toxoplasma gondiiin horses in Changji Hui Autonomous Prefecture,Xinjiang, northwestern China.Braz. J. Vet. Parasitol.,Jaboticabal. (2015); ISSN 1984-2961 (Electronic).
6-Mehdi T, Mohammad S, Rahman A, Shahram K, Abdollah R, Anahita R. Seroprevalence of Toxoplasma gondii and Neospora spp. Infections in Arab Horses, Southwest of Iran.Jundishapur J Microbiol., (2015); 8(3):149-154 .
7-Mohammed B, Dauda M, Abubakarand Z, Adamu G. Prevalence of Brucella antibodies in horses (Equus caballus) in Jalingo, Taraba State, Nigeria. Journal of Public Health and Epidemiology. (2016); 8(7):111-114.
8-Mohammed B, Dauda M. Seroprevalence of horse (Equus caballus) brucellosis on the Mambilla plateau of Taraba State, Nigeria.J Equine Sci. (2016); 27(1):1–6.
9-Özgür C, Fatih B, Aliye G, Ahmet Ü, Salih O. Seroprevalence of brucellosis in horses in Kars Ardahan provinces of Turkey where ruminant brucellosis is endemic and prevalent. Kafkas Univ Vet Fak Derg,: (2013); (3)19:541-544.
10-Yahya T, Mohammad M, Gholamreza M, Gholamreza M. Prevalence of brucellosis in horse North-East of Iran.J.E.V.S., (2010); 30 (7): 376-378.
11-Navab G, Ali R. Evaluation of prevalence of brucellosis in horse Hamadan of Iran.IJACS., (2013); 5(6): 603-605.
12-Masatani T, Takashima Y, Takasu M, Matsuu A, Amaya T. Prevalence of anti-Toxoplasma gondii antibody in domestic horses in Japan. Parasitol Int., (2016); 65(2):146-50.
13-Saqib M, Hussain MH, Sajid MS, Mansoor MK, Asi MN, Fadya A, Zohaib A, Muhammad G, Ullah A. Sero-epidemiology of equine toxoplasmosis using a latex agglutination test in the three metropolises of Punjab Pakistan. Tropical Biomedicine. (2015); 32(2):276–285.
14-Altaee IA, Al-Ani JM, Al-Rubaie HM. Seroprevalence of Toxoplasmosis in horses and animal handlers in Baghdad city. Al-Anbar Journal of Veterinary Sciences. 7(Issue:2): (2014); 28-34.
15-Alshahery MN, Mansour RS. Detection of Toxoplasma gondii antibodies in horses in Mosu Iraq.Iraqi Journal of Veterinary Sciences. (2012); 26 (Supplement II) :39-41.
16-Radostits O, Gay C, Hinchcliff K, Constable D. A Text book of the diseases of cattles,sheep,pigs,goats and horses. 10th ed London. Saunders Elsevier. Edinburgh. (2007); Pp (966-975).
How to Cite
SALEEM AMIN HASSO, Jalil Abed Gatie and. Serological investigation of Brucella spp. and Toxoplasma gondii in horses by using rose Bengal and toxoplasma latex tests in Dhi-Qar province, Iraq. AL-Qadisiyah Journal of Veterinary Medicine Sciences, [S.l.], v. 16, n. 1, p. 5-8, nov. 2017. ISSN 2313-4429. Available at: <>. Date accessed: 19 mar. 2018. doi: