Molecular detection and phylogenetic analysis of Coxiella burnetii in goats milk

  • Hayder N. Ayyez Unit of Zoonotic Diseases, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq


   Over the last years, the number Coxiella burnetii infections increased throughout the wourld. A molecular detection and phylogenetic analysis were performed in Al-Diwaniayh city in which the first phylogenetic documentation of C.burnetii reported in this area with accession numbers KY576802.1, KY576803.1, KY576804.1 and KY576805.1 Molecular detection and phylogenetic analysis of C.burnetii targeting the transposase gene partial sequence  was done on the milk samples from 50  apperently healthy goats. DNA sequence was amplified in 6 out of 50 (12%) milk samples collected from different regions of Al Diwaniya city. Four of these amplicons were submitted to sequences analysis and gave 99% nucleotides sequence similarity. Also the amplicon sequences of local strain were compared with global C.burnetti sequences in NCBI which revealed  close related to NCBI-Blast C.burnetii transposase gene of Indian (AB84899.1) and Brazilian (JF970261.1) strains, whereas there was genetic variation to NCBI-Blast C.burnetii transposase gene for USA (DQ379976.1), Portugal (EU009657.1) and Taiwan (EU000273.1). This work confirm goats infection  and shedding the C. burnetii in Al-Diwaniayh, Iraq and determined the phylogenetic tree of local strain of this bacterium.


1-Raoult D, Marrie T, Mege J. Natural history and pathophysiology of Q fever. Lancet Infect Dis, (2005); 5:219-226.
2-Ming-Yang Y, Si-Xian Q, Qi-Dong T, Sheng- Yong F, Guang-Xue L, Dong- Hui Z, Xing-Quan Z. First report of C.burnetti seroprevelance in Tibetan sheep in China. Vector-Borne and Zoonotic Diseases; (2015);15(7):419-422.
3-Domenico M, Curini V, Massis F, Provvido A, Scacchia M, Camma C. Coxiella burnetii incentral italy Novel genotype are circulating in cattle and goats. (2014); V 14, No. 10.
4-Agarholm JS. Coxiella burnetii associated reproductive disorder in domestic animals a critical review. Acta Vet Scand (2013); 55:13.
5-Setiyono A, Ogawa M, Cai Y, Shiga S, Kishimoto T, Kurane I. New criteria for immunofluorescence assay for Q fever diagnosis in Japan. J Clin Microbiol; (2005); 43:5555-5559.
6-Borriello G, Iovane G, Galiero G. La febbre Q negli animali domestici. Large Animal Review; (2010); 16: 273-283.
7-ECDC (Eurooean Centre for Disease Prevention and Control). Panel with Representatives from the Netherlands, France, Germany, United Kingdom, United States of America. Risk assessment on Q fever. ECDC Technical Report;( 2010); 40 pp.
8-Zhang GQ, To H, Yamaguchi T, Fukushi H, et al. Differentiation of Coxiella burnetii by sequence analysis of the gene (com1) encoding a 27-kDa outer membrane protein. Microbiol Immunol. (1997); 41:871-877.
9-Khalifa NO, Elhofy FI, Fahmy HA, Sobhy MM, Agag MA. Seropervelance and molecular detection of Coxiella burnetii infected in sheep, goats and human in Egypt. (2016);Vol 2 No. 1-7.
10-Markey B, Leonard F, Archambault M, Cullinane A, Maguire D. Clinical Veterinary Microbiology. (2013);Second edition
11-BY, Seo MG, Lee SH, Byun JW, Oem JK, Kwak D. Molecular and serologic detection of Coxiella burnetii in native Korean goats (Capra hircus coreanae). (2014); Vet Microbiol.
12-Abbasi S, Farzan R, Momtaz H. Molecular detection of Coxiella burnetii in goat bulk milk samples in some provinces of Iran. (2011); 10 (80): 18513-18515.
13-Guatteo R, Beaudeau F, Joly A, Seegers H. Assessing the within-herd prevalence of Coxiella burnetii milk shedder cows using a real-time PCR applied to bulk tank milk. Zoonoses Puplic health, (2007);54: 191-194.
How to Cite
AYYEZ, Hayder N.. Molecular detection and phylogenetic analysis of Coxiella burnetii in goats milk. AL-Qadisiyah Journal of Veterinary Medicine Sciences, [S.l.], v. 16, n. 1, p. 79-83, nov. 2017. ISSN 2313-4429. Available at: <>. Date accessed: 19 mar. 2018. doi: