Genotyping of Brucella melitensis isolated from human and sheep in Iraq
Brucellosis is a widespread endemic zoonotic disease as well as significant impact on human health together with ruminant’s manifests as abortions or other reproductive problems in different animal’s species. A specific sensitive PCR and DNA sequencing technique employed in this study to provide the first Iraqi profile about B. melitensis in Genebank to overcome the determinates posed by the others accurate diagnostic methods like isolation and serotyping. In Women's Maternity and Children Hospital, and Gynecology Outdoor Patient (OPD) in the city One hundred twenty two (122) samples (107 serum and 15 aborted fetus) collected from a women have a history of abortion and either aborted fetus, serum tested directly with rose Bengal while aborted fetus submitted to culturing. Seventy four (74) blood samples collected from different ewes with abortion history tested with Rose Bengal test conducted the positive cases to PCR then DNA sequencing. Out of 196 samples 6 samples (2 human and 4 sheep) were positive for PCR technique, while only 3 partial gene sequenced samples were identified as B. melitensis revealed three different biovars available under accession number (KX793714.1, KX793715.1, and KX793716.1) in Genebank A1, A2 strains isolated from sheep and A3 human strain. B. melitensis was the only species detected, ensuring its highest zoonotic potential in Brucella genus. A1 and A2 Sheep isolate were shown closed related to NCBI-Blast Brucella melitensis biovar 3 (DQ086122.1). Whereas, the Brucella spp. A3 Human isolate was shown closed relation to NCBI-Blast Brucella melitensis biovar 1 (DQ086119.1) and ( DQ086121.1).
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