Phylogenetic tree analysis study of Lumpy skin disease virus based envelope protein P32 gene in Al-Qadisiyah province, Iraq

  • Khalefa Ali Mansour Department of Internal Veterinary Medicine, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq.


   In this study, fifty samples from nodules of skin tissue collected from cattle were clinically supposed to be infected with lumpy skin disease (LSD) from deferent area in Al-Qadisiyah province .These specimens were provided to PCR assay. The endpoint of conventional   PCR assay was sent to sequence analysis. The results revealed that thirty-nine samples out of fifty were positive to lumpy skin disease virus at 752bp PCR product of p32 gene, the sequence analysis of five positive samples were done as a confirmative diagnosis to PCR assay. The sequences of these five samples can be found under the accession numbers (KR066462.1,KR066463.1,KR066464.1,KR066465.1 and KR066466.1) at NCBI-Gen Bank submission. In conclusion; the sequence analysis of five local isolate of lumpy skin disease virus was close related to NCBI-Blast reference lumpy skin disease virus isolate (KU720359.1) and Kurdistan isolates, whereas others "Turkish and Egyptian isolates"  were different.


1-Fenner F. Poxviruses. In B.N. Fields, D.M. Knipe, and P.M. Howley (ed.), Fields virology. Lippincott-Raven, Philadelphia, Pa, (1996); 2673-2702.
2-Esposito JJ, Fenner F. Poxviruses, In: Fields Virology. Field, B.N., D.M. Knipe, P.M. Howley, R.M. Chanock and J.L. Melnick et al., (Eds.). Lippincott, Williams and Wilkins, Philadelphia, Pa. (2001).
3-Davies FG. LSD- An African Capripox virus disease of cattle. Br. Yet. J., (1991);147: 489-503.
4-JAW Coetzer, Dr. EevaTuppurainen. Lumpy skin disease. Oxford University Press Southern Africa, (2004); 2:1268-1276.
5-Tuppurainen E, Oura C. Review: Lumpy skin disease: An emerging threat to Europe, the Middle East and Asia. Trans-boundary and Emerging Diseases, (2012); 59, 40-48.
6-Al-Salihi K, Hassan I. Lumpy skin disease in Iraq: Study of the disease emergence. Transboundary and Emerging Diseases, ( 2015); 62 , 457-462.
7-Prozesky L, Barnard BJH. A study of the pathology of LSD in cattle. Onderstepoort. J. Vet. Res, (1982); 49: 167-175.
8-Hamoda FK, Aboul-Soud EA, Magda MS, Shahein MA, Michael A, Daoud AM. Field and laboratory studies on lumpy skin disease. J. Egypt. Vet. Med. Ass., (2002); 625: 183-199.
9-Zangana, I, M Abdullah. Epidemiological, clinical and histopathological studies of lamb and kid pox in Duhok, Iraq. Bulgarian Journal of Veterinary Medicine,(2013).16,133-138.
10-Buller RM, Arif BM, Black DN, Dumbell KR, Esposito JJ, Lefkowitz EJ, McFadden G, Moss B, Mercer AA, Moyer RW, Skinner MA, Tripathy DN. Poxviridae. In: Fauquet, C.M., Mayo, M.A., Maniloff, J., Desselberger, U., Ball, L.A. (Eds.), Virus Taxonomy: Eight Report of the International Committee on the Taxonomy of Viruses. Elsevier Academic Press, Oxford, (2005); pp. 117–133.
11-Fauquet CM, MA Mayo, J Maniloff, U Desselberger, LA Ball. Virus Taxonomy: VIIIth Report of the International Committee on Taxonomy of Viruses, Academic Press. (2005).
12-El-Kenawy AA, El-Tholoth MS. Isolation and identification of lumpy skin disease virus from cattle on chorioallantoic membranes (CAMs) of fertile eggs. Proceedings of the 6th International Science Conference, April, 22-23, Mansoura, (2009); PP: 115-133.
13-Rashid PMA, Baba Sheikh MO, Raheem ZH, Marouf AS. Molecular characterization of lumpy skin disease virus and sheeppox virus based on P32 gene. Bulg. J. Vet. Med. (online first). (2016).
14-Heine HG, Stevens MP, Foord A, Jand Boyle DB. A capripoxvirus detection PCR and antibody ELISA based on the major antigen P32, homologue of the vaccinia virus H3L gene. Journal of Immunological Methods, (1999); 227: 187-196.
15-Khalefa AM, Hassan HN, Asaad j. Molecular and Clinical investigation of lumpy skin disease in cattle in AL .Qadissiyia province. Kufa Journal For Veterinary Medical Sciences. (2015); 6 :8-14.
16-El-Bagoury GF, El-Nahas EM, El-Habbaa AS, Abdel-Nour ET. Isolation and Molecular Characterization of Lumpy Skin Disease Virus from Kalubia Governorate. Benha Vet. Med. J., Vol. 20, No.2. (2009).
17-IRELAND DC, BINEPAL YS. Improved detection ofcapripoxvirus in biopsy samples by PCR. Journal of VirologicalMethods, (1998); 74:1-7.
18-Tulman E, Afonso C, Lu Z, Zsak L, Sur JH, Sandybaev N, Kerembekova U, Zaitsev V, Kutish G, Rock D. The genomes of sheeppox and goatpox viruses. Journal of Virology, (2002);76, 6054-6061.
19-Beard P, Sugar S, Bazarragchaa E, Gerelmaa U, Tserendorj S, Tuppurainen E, Sodnomdarjaa R. A description of two outbreaks of capripoxvirus disease in Mongolia. Veterinary Microbiology, (2010); 142, 427-431.
20-Orlova ES, Shcherbakov AV, Diev VI, et al. Differentiation of capripoxvirus species and strains by polymerase chain reaction.. Molecular Biology, (2006); 40 (1): 158-164.
21-Lamien CE, Goff CLe, Silber R, Wallace DB, Gulyaz V, E Tuppurainen, H Madani, P Caufour, T Adam, M El Harrak, AG Luckins, E Albina, Diallo A. Use of the Capripoxvirus homologue of Vaccinia virus 30 kDa RNA polymerase subunit (RPO30) gene as a novel diagnostic and genotyping target: development of a classical PCR method todifferentiate Goat poxvirus from Sheep poxvirus. Vet Microbiol, (2011); 21, 149: 30-9.
22-Zhu X, Yang F, Li H, Dou Y, Meng X, H. Li, X. Luo& X. Cai. Identification and phylogenetic analysis of a sheep pox virus isolated from the Ningxia Hui Autonomous Region of China. Genetics and Molecular Research, (2013);12, 1670-1678.
23-Fulzele S, RK Singh, M Hosamani, B Mondal, MP Yadav. Evaluation of duplex PCR and PCR-RFLP for diagnosisof sheep pox and goat pox. International Journal of Tropical Medicine, (2006);1,66-70.
24-El-Nahas EM, El-Habbaa AS, El-bagoury GF, Mervat EI Radwan. Isolation and Identification of Lumpy Skin Disease Virus from Naturally Infected Buffaloes at Kaluobia, Egypt. Global Veterinaria. (2011);7 (3): 234-237,
25-Stram Y, Kuznetzova L, Friedgut O, Gelman B, Yadin H, Rubinstein-Guini M. The use of lumpy skin disease virusgenome termini for detection and phylogenetic analysis. Journal of Virological Methods. (2008); 151: 225-229.
26-Black DN, Hammond JM, Kitching RP. Genomic relationship between capripoxviruses. Virus Res., (1986). 5:277-292.
27-Tulman ER, Afonso CL, Lu Z, Zsak L, Kutish GF, Rock DL. Genome of Lumpy Skin Disease Virus. Journal of Virology, Vol. (2011); 75 (15): 7122-7130.
How to Cite
MANSOUR, Khalefa Ali. Phylogenetic tree analysis study of Lumpy skin disease virus based envelope protein P32 gene in Al-Qadisiyah province, Iraq. AL-Qadisiyah Journal of Veterinary Medicine Sciences, [S.l.], v. 16, n. 1, p. 106-111, nov. 2017. ISSN 2313-4429. Available at: <>. Date accessed: 20 sep. 2018. doi: