Phylogenetic tree analysis study of Lumpy skin disease virus based envelope protein P32 gene in Al-Qadisiyah province, Iraq

  • Khalefa Ali Mansour Department of Internal Veterinary Medicine, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq.

Abstract

   In this study, fifty samples from nodules of skin tissue collected from cattle were clinically supposed to be infected with lumpy skin disease (LSD) from deferent area in Al-Qadisiyah province .These specimens were provided to PCR assay. The endpoint of conventional   PCR assay was sent to sequence analysis. The results revealed that thirty-nine samples out of fifty were positive to lumpy skin disease virus at 752bp PCR product of p32 gene, the sequence analysis of five positive samples were done as a confirmative diagnosis to PCR assay. The sequences of these five samples can be found under the accession numbers (KR066462.1,KR066463.1,KR066464.1,KR066465.1 and KR066466.1) at NCBI-Gen Bank submission. In conclusion; the sequence analysis of five local isolate of lumpy skin disease virus was close related to NCBI-Blast reference lumpy skin disease virus isolate (KU720359.1) and Kurdistan isolates, whereas others "Turkish and Egyptian isolates"  were different.

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Published
2017-11-19
How to Cite
MANSOUR, Khalefa Ali. Phylogenetic tree analysis study of Lumpy skin disease virus based envelope protein P32 gene in Al-Qadisiyah province, Iraq. AL-Qadisiyah Journal of Veterinary Medicine Sciences, [S.l.], v. 16, n. 1, p. 106-111, nov. 2017. ISSN 2313-4429. Available at: <http://qu.edu.iq/journalvm/index.php/vm_journal/article/view/45>. Date accessed: 24 june 2018. doi: https://doi.org/10.29079/vol16iss1art45.