Impact of Fascioliasis on circulating physiological biomarkers of stress and inflammation in cattle

  • Shaimaa Abbas Sabeeh College of Veterinary Medicine, University of Al-Qadisiyah, Iraq
  • Esraa Taher Muslim College of Veterinary Medicine, University of Al-Qadisiyah, Iraq.
  • Asseel A. Saeed College of Veterinary Medicine, University of Al-Qadisiyah, Iraq.


This study was conducted for verifying the effect of liver worm sickness  (Fascioliasis) on the general health of the animal, The hematocrits levels, (RBCs) count, Haemoglobin concentration ( Hb ) and packed cell volume  (PCV) were also determined  in the two groups of beef  infected and non-infected with Fascioliasis  the inflammatory parameter in liver :glutamic oxaloacetic transaminase (GOT) and  glutamate pyruvate transaminase( GPT) were determined  in the beef with moderate Fascioliasis and  The results of this study were compared between infected animals and non-infected animals. A few have been taken oxidative stress: Superoxide Dismutase (SOD), Glutathione (GSH), Malondialdehyde (MDA) and catalase enzyme (CAT).  In both groups, there were some significant contrasts in the levels of GSH, MDA, SOD, CAT and GOT, GPT. For CAT there was a significantly accelerated in its levels in the infected beef in comparison with the non-infection beef.  While GSH and MDA there was a decrease in the level of GSH and an accelerated in the level of MDA in compared to non-infected beef respectively. SOD activity was decreased in blood serum of infected beef comparison with non-infected. The findings showed the beef infected with F. spp. were under oxidative stress. In inflammatory parameters were some significant differences in the levels of (GOT, GPT), its showed accelerated in levels with infected animals and normally in non-infected animals. This research reinforces that animals infected with trematodes, which may have effects on the overall health of animals, will be under oxidative stress and inflammation.


1-Marquardt WC, RS Demaree, RB Grieve. Parasitology and Vector Biology. 2 led. London: Harcourt Academic press, (2000); pp: 273-279.
2-Urquhart GM, JL Amour, AM Dunn, FW Jennings. Veterinary Parasitology Oxford: Blackwell publishing, (1996); Pp 103-112.
3-Taylor MA, RL Coop, RL Wall. Veterinary parasitology. 3 died. Oxford: Blackwell Publishing, (2007); pp: 85-87
4-Hunter A. Fascioliasis in animal health. Specific Disease in Tropics: 1- ed. London: CT Macmillan (1994); 2:149-154.
5-Kassai T. Veterinary Helminthology. University of science, oxford: Butterworth Heinemann. (1999); pp: 9.
6-Behm CA, NC Sangster. 23. Pathology, Pathophysiology and clinical aspects of fascioliasis. 2ed. Welling ford: CABI publishing, (1999); pp: 185-224.
7-Cohn VH, Lyle J. Volumetric Assay for Glutathione. Analytical Biochemistry (1964); 14: 434-440.
8-Lind C, Cadenas E, Hoshstein P, Ernster L. DT. Diaphoresis purification. Methods Enzymology (1990); 186: 287-301.
9-Simesen MG, Nansen P. Serum g-glutamyl Trans peptidase (g-GT) and aspartate aminotransferase (Asp AT) activities in adult cattle with chronic Fasciola hepatica infection. Acta Vet Scan (1974); 15: 239 -243.
10-Vengust G, Klinkon M, Bidovec A, Vengust, A. Fasciola hepatica: effects on blood constituents and liver minerals in fallow deer (Dama dama). Veterinary Parasitology (2003); 112: 51-61.
11-Matanovic K, Severin K, Martinkovic F, Simpraga M, Janicki Z, Barisic J. Haematological and biochemical changes in organically farmed sheep naturally infected with Fasciola hepatica. Parasitology Research (2007); 101: 1657-1661.
12-Saleh MA. Circulating oxidative stress status in desert sheep naturally infected with Fasciola hepatica. Veterinary Parasitology (2008); 154: 262-269.
13-Ashaolu JO, Ukwenya VO, Okonoboh AB, Ghazal OK, Jimoh AAG. Effect of momosodium glutamate on haematological parameters of Winstar rats. International Journal of Medicine and Medical Sciences (2011); 3: 219-222.
14-Berry CI, Dargie JD. Pathophysiology of ovine Fascioliasis: the influence of dietary protein and iron on the erythrokinetics of sheep experimentally infected with Fasciola hepatica. Veterinary Parasitology (1978); 4: 327-339.
15-Howlader MMR, Begum S, Islam NK, Hai MA, Hossain MG. Further observation on the packed cell volume and hemoglobin concentration in cattle naturally infected with Fasciola gigantica. Bangladesh Journal of Veterinary Medicine (2004); 2: 125 -127.
16-Deger Y, Ertekin A, Deger S, Mert H. Lipid Peroxidation and Antioxidant Potential of Sheep Liver Infected Naturally with Distomatosis. Turkish Society for Parasitology, (2008); 32: 23-26.
17-Loyacano AF, JC Williams, J Guriey AA Derosa. Effect of gastrointestinal nematode and liver fluke on weigth gain and reproductive performance of beef heifers. Vet. Parasitol. (2002); 107, 227-234.
18-Caicedo Rivas RE, J Dimas Ros, A Torres Beltrn, SV Martnez Badillo, R Pérez Avilés, JS Hernndez Zepeda y R. Reséndiz Martnez. La fasciolosis como ecopatologa que afecta la productividad ganadera en el Estado de Puebla, México. En XI Simposio Iberoamericano sobre Conservaciَn y Utilizaciَn de Recursos Zoogéneticos. Joao Pessoa-Paraba, Brasil. ISSN: 2179-1961. (2010); Pp.395-399.
19-Ortega V, RE Caicedo, JS Hernndez, R Resendiz, y A. Ramrez. (2007). Fisiopatologa hepلtica en bovinos(Bos taurus X Bos indicus) con fasciolosis en el Estado de Puebla. Memorias del L Congreso Nacional de Ciencias Fisiolَgicas, Puebla, Pue., México.
20-Chirinos ARy, NI De Chirinos EvaluaciÙŽn de los efectos de la distomatosis heptica bovina sobre la eficiencia reproductiva y producciÙŽn lechera. Rev. Cientf. FCV-LUZ. III, (1993); 35-45.
21-Rahman I, Biswas SK, Kode A. Oxidant and antioxidant balance in airways and airway diseases. European Journal of Pharmacology (2006); 533: 222-239.
22-Kolodziejczyk L, Siemieniuk E, Skrzydlewska E. Antioxidant potential of rat liver in experimental infection with Fasciola hepatica. Parasitology Research (2005); 96: 367-372.
23-Ji LL, Stratman W, Lardy HA. Antioxidant enzyme systems in rat liver and skeletal muscle.Influences of selenium deficiency, chronic training and acute exercise. Archives of Biochemistry and Biophysics (1988); 263: 150-160.
24-Benzer F, Ozan TS. The status of lipd peroxidation, antioxidant enzymes and nitric oxide in sheep infected with Fasciola hepatica. Turkish Journal of Veterinary Animal Science (2003); 27: 657-661.
25-Siwela AH, Mambvura CI, Masanganise KE, Dube S. Oxidative stress associated with nematode infections in sheep: a preliminary study. International Journal of Biological and Chemical Sciences (2010); 4: 235-240.
26-Fabian E, Poloskey P, Kosa L, Elmadfa I, Rethy LA. Activities of antioxidant enzymes in relation to oxidative and nitrosative challenges in childhood asthma. Journal of Asthma (2011); 48: 351-357.
27-Veldhoen M, Hocking RJ, Atkins CJ, Locksley RM, Stockinger B. TGF-beta in the context of an inflammatory cytokine milieu supports de novo differentiation of IL-17 producing T cells. Immunity (2006), 24: 179-189.
28-Galtier P, Cambon-Gros C, Fernandez Y, Deltour P, Eeckhoutte C, Hoellinger H. Fasciola hepatica: liver microsomal membrane functions in host rat. Exprimental Parasitology, (1994); 78: 175-182.
29-Galtier P, Cambon-Gros C, Fernandez Y, Deltour P, Eeckhoutte C, Hoellinger H. Fasciola hepatica: liver microsomal membrane functions in host rat. Exprimental Parasitology, (1994); 78: 175-182.
30-Maffei FR, Carini M, Aldini G, Ceserani R, Ceserani I, Cavaletti E, Vederio L. Ef icacy of glutathione for treatment of Fascioliasis, an investigation in the experimentally infected rat. (1993); Arzneim-Forsch/Drug Research43:455-460.