Comparative study on locally produced bivalent inactivated Newcastle disease vaccine in broiler chicks
The study included titration of the Newcastle disease virus strains before the preparation of the vaccines used in this experiment, by detection of EID50 for these strains. This study regarded the first one in the country in preparation double strain oily Newcastle disease virus vaccine. The immune responses for this vaccine were compared with those of single strain NDV vaccine. The same vaccination program was used for all groups included in this study in which live attenuated) Lasota strain) vaccine given at one day old as an installation by eye and nostril, and subcutaneous injection of oily vaccine at the neck region. A total of 120 commercial broiler chicks was divided into fourth groups, (1th) group vaccinated with live attenuated vaccine and bivalent(B1, Lasota )oily vaccine while the (2th) group vaccinated with live attenuated vaccine and (Lasota)oily vaccine and (3th) group vaccinated with live attenuated vaccine and (AG68 )oily vaccine, (4th) group C was left unvaccinated as control, the Immune response for all groups was measured at( 1) day old, (7), (14), (21),and ( 35) days old by hemagglutination test (HI) and Enzyme liked immunosorbent assay (ELISA). The results showed gradual recreation of immune response titer at 7,14 and 21 days post-vaccination intervals with non-significant differences (P> 0.05) among vaccinated groups at (7,14) days and significant difference between vaccinated groups and control group (P>0.05) at (21) days old.T he third group reflected better titer among vaccinated groups, and the result also shows increased antibodies titer at (35) day in comparison with antibodies titer at (14,21) days intervals. With statistical differences (P>0.01) among (third) group and second, and control group). However all groups gave higher titer than the control group (P>0.01) and the result of (HI) test agreement with (ELISA) test while the protection percentage was measured by challenge test ,it was found to be ( 100 % ) for group (3th) vaccinated with live attenuated vaccine and bivalent(B1, Lasota )oily vaccine, (92%) for the second groups (2th) (96%) for the first groups (1th) whereas control group (4th) gave (10%) protection. This study indicates the efficacy of prepared ND vaccines (B1, Lasota) as compared with monoclonal vaccines (B1, Lasota) and the high immunosuppressive capacity of the oily vaccine increased with virulence.
2-Akkar Adel Saber. Comparison of different vaccination programs to vaccinate chicken broilers with Newcastle oil vaccine. Master Thesis, Faculty of Veterinary Medicine, University of Baghdad, (2004).
3-OIE. Newcastle disease. The Center for Food security and Public Health. Collage of veterinary medicine, Iowa state university,(2004).
4-Palya V.Manual for the productin of Mareks disease,Gumboro disease and inactivation Newcastel disease vaccines.Phylaxia Veterinary Biological Company. Budapest, Hungary. F.A.O.R. (1991).
5-Grimes E Sally. A basic laboratory manual for the small- scaie production and testing of I-2 Newcastle disease vaccine. Australian Center for International Agricultural Research. (2002).
6-Brugh M, Stane HD, Lupton HW. Comparison of inactivated Newcastle disease viral vaccines containing different emulsion adjuvants. Am. J. Vet. Res. (1983); 44: 72-75
7-Yong M, Grimes R, Spradbrow P, Dias P, Siwa A, Lobo Q. Controling Newcastle disease, Al aboratry manual in village chickens. Australian Center for International Agriculture Research http://www.aciar.gov.au. (2002).
8-Saadi Majid Younis. A Comparative Study to Immunize Chicken Embryos against Newcastle Disease. Master Thesis, Faculty of Veterinary Medicine, University of Baghdad. Mokhtari, (2003).
9-Jamil Abbas Ahmed. Immune response to vaccinated chickens with domestically produced Newcastle vaccines. Master Thesis, Faculty of Veterinary Medicine, University of Baghdad. (1996).
10-Borland LJ, Allan WH. Laboratory tests for comparing live lento genic Newcastle disease vaccines. Avian Pathology. (1980); 9:45-59.
11-Degefa T, Dadi L, Yami A, Marian G, Nassir M. Technical and economic evaluation of different methods of Newcastle disease vaccine. Administration. J. Vet. Med. Aphysiol. Pathol. Clin. Med. (2004); 51(7-8): 365-369.
12-Erhard MH, Schmidt P, Zinsmeister P, Hofmann A, Munster U, Kaspers B, Wiesmuller KH, Bessler WG, Stangassinger M. Adjuvant effects of various lipopeptides and interferon- y on the humoral immune response of chickens. Poult. Sci. (2000); 79: 1264-1270.
13-Huang Z, Panda A, Elankumaran S, Govindarajan D, Rockemann DD, Samel SK. The Hemagglutinin – neuraminidase protein of Newcastle disease virus determines tropism and virulence. J. Virol. (2004); 78(8): 4176-4184.
14-Jacob JP, Butcher GD, Mather FB. Vaccination. Of small poultry flocks. University of Florida. Institute of food and Agricultural Sciences. Poultry view. Com. Vaccination. htm. Site http://hammock.ifas.ufl.edu. (2001).
15-Mass RA, Komen M, Van diepen M, Oei HL, Claassen IJ. Correlation of haemagglutinin neuraminidase and fusion protein content with protective antibody response after immunization with inactivated Newcastle disease vaccines. Vaccine. (2003); 12: 3137-3142.
16-Panshin A, Shinmanter E, Weisman Y, Orvell C, Lipkind M. Antigenic heterogenity among the field isolates of virus in relation to the vaccine strain. I- Studies on viruses isolated from domestic bird. Comp Immunol. Microbiol Infect Dis. (2002); 25(3): 173- 185.
17-Saville P. Newcastle disease and avian influenza. Animal Health Advisory Leaflet, South Pacific Commission. (1996); 8.
18-Van-Jhh E, Witenbury NN, Jasper D, AN-Wittonbury N.An ulster2c strain-derived newcastel disease vaccine:efficacy and excrection of maternally immune chicken. Avian Dis. (1997); 20:260-267.
19-Winterfield RW, Dihillon AS. Comparative immuno response from vaccination chickens with Lentogenic Newcastle disease virus strains. Poult. Sci. (1981); 60:1195-1203.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright: All manuscripts published by Al-Qadisiyah Journal of Veterinary Medicine Sciences are under the terms of the Creative Commons Attribution License. This permits anyone to copy, distribute, transmit and adapt the work provided the original work and source is appropriately cited. Copyright on any open access article published by Al-Qadisiyah Journal of Veterinary Medicine Sciences is retained by the author(s).
Note: The journal is not allow any scientific misconduct in any published research article. Any article exceeds the permissible ratio of plagiarism is rejected. However, as a publisher will follow strict scientific guidelines and advice to retract or erratum of any article at any time if found scientific misconduct or errors in any articles. Authors are responsible for obtaining permission from copyright holders for reproducing any illustrations, tables, figures or lengthy quotations previously published elsewhere.