A new approach for estrus induction in Arabian mares suffering from ovarian inactivity

  • Karam K.M Department of Theriogenology, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq
  • El-Belely M.S Department of Theriogenology, Faculty of Veterinary Medicine, University of Cairo-Giza Egypt.
  • Ismail S.T Animal reproduction research institute-Giza-Egypt
  • Fadel M.S Department of Cairo Mounted, Cairo Police, Cairo, Egypt
  • Abu Atiah E.F Department of Theriogenology, Faculty of Veterinary Medicine, University of Cairo-Giza Egypt.

Abstract

Twenty-six Arabian mares affected with ovarian inactivity, as monitored by the clinical examination and ultrasonographical scanning, were selected during December and January. These animals were subjected to a new protocol of sequential hormonal injections as a trial for estrus induction, progesterone (300 mg/day) was injected on days 1,4,7,10 and 14, Estradiol benzoate (15mg/day) on days 6 and 9, and GnRH (0.01mg/day) on days 18, 20, and 22 of the treatment regimen. Anestrus induction response of 80.77% (21/26) was observed following hormonal treatment. The onset time of post-treatment was short (66.48 hours) and more synchronized. The responded group showed significant (P<0.05) increase in follicular size from the day -3 (3.26 cm) until reaching its maximum size at the pre-ovulatory day (4.48cm). This was accompanied by continuous significant (P<0.05)decrease in the serum concentrations of progesterone and increase in the levels of serum estradiol-17β from the day -3 (0.42 ng/ml and 56.48 pg/ml, respectively). The minimum level of progesterone (0.28 ng/ml) was detected at day 0 of ovulation, whereas the maximum level of estradiol-17β was observed at the preovulatory day (89.22 pg/ml). However, 28.57% (6/21) of the responded mares did not develop functional corpus luteum so confirmed by functional progesterone estimation. Reasons for failure of 19.23% mares (5/26) to respond to hormonal treatment could be due to the small size of emerging follicles at the end of treatment (1.05  vs 1.82 cm ) as well as the older ages of these animals (15.36 vs 8.87 years old) compared to the responded group.

References

1-Alt MO, Cetin H, Zonturlu AK. Effect of progesterone impregnated device combined with prostaglandin F2 alpha on the oestrus cycle and fertility in mares. Saglk, Bilimleri Dergisi, Firat Universitesi Veteriner, (2004); 18 (1): 55-60.
2-Amer HA, Shawki G, Ismail R. Profile of steroid hormones during estrus and early pregnancy in Arabian mares. Slov Vet.Res.: (2008); 45(1)25-32.
3-Arbeiter K, Barth H, Jochle W. Observation on the use of progesterone intra-vaginally and of deslorelin in acyclic mares for induction of ovulation. Journal of Equine Veterinary Science, (1994); 14 (1): 21-25.
4-Ataman MB, Gunay A, Gunay U, Baran A, Uzman M. Oestrus synchronization with progesterone-impregnated device and prostaglandinF2alpha both combined with human chorionic gonadotropin in transitional mares. Revue de Medicine Veterinaire, (2000); 151 (11): 1031-1034.
5-Card C, Green J. Comparison of pregnancy rates by week from stallion exposure and overall pregnancy rates in pasture-bred mares synchronized with CIDR and/ or prostaglandin F2 alpha. In 50th Annual conference American Association of Equine Practicener, Denver, Colorado (2004); 4-6 Dec.
6-Evans MJ, Irvine CHG. Induction of follicular development and ovulation in seasonally acyclic mares using gonadotropin releasing hormone and progesterone. Journal of Reproduction and Fertility Supplement, (1979); 27: 113-121
7-Fitzgerald BP, Affleck KJ, Permstein R, Loy RG. Investigation of the potential of LHRH or an agonist to induce ovulation in seasonally anestrus mares with observations on the use of agonist in problem acyclic mares. J. Reprod. Fert., suppl. (1987); 35, 683-684.
8-Ginther OJ, Beg MA, Gestal MO, Gestal EL. Follicle dynamics and selection in mares. Animal reproduction, (2004); 1:45-63
9-Ginther OJ, Beg MA, Gestal EL, Gestal MO, Baerwald AR, Pierson RA. Systemic concentrations of hormones during the development of follicular waves in mares and women: a comparative study. J.Reprod. (2005); 130(3) 379-388.
10-Ginther OJ, Utt MD, Beg MA, Gestal EL, Gestal MO. Negative effect of Estradiol on Luteinizing hormone throughout the ovulatory luteinizing hormone surge in mares: Biology of reproductive (2007); 77,543-550.
11-Hyland JH, PJ, Wright IJ, Clark RS, Carson DA Langfield, LB Jeffcott. Infusion of gonadotrophin-releasing hormone (GnRH) induces ovulation and fertile oestrous in mares during seasonal anoestrus. J. Reprod. Fertil. (Suppl.) (1987); 35:211.
12-Johnson AL. Induction of ovulation in anestrous mares with pulsatile administration of gonadotropin-releasing hormone. American Journal of Veterinary Research, (1986); 47: 983-986.
13-Kumar R, Phogat JB, Mann JS, Singh U, Sharma AK. Estrus induction and fertility response in anestrus mares with exogenous progesterone releasing device (CIDR-B) during late breeding season. Biology and Medicine, 3 (2) special issue (2011); 359- 364
14-Lubbecke M, Klung E, Hoppen O, Jochle W. Attempts to synchronize oestrus and ovulation in mares using progesterone (CIDR-B) and GnRH analog, deslorelin. Reproduction in Domestic Animal, (1994); 29 (4): 305-314.
15-Martin JL, Saltiel A, Evans JW. Progesterone synthesis by different types of corpora lutea during the first 198 days of pregnancy. J. Equine Vet. Sci. (1989); 9:84.
16-Newcombe JR, Wilson MC. The use of progesterone releasing intra-vaginal devices to induce oestrus and ovulation in anoestrus standard bred mares. Australian Journal of Equine Practice, (1997); 19 (6): 13-21.
17-Newcombe JR, Handler J, Klug E, Meyers PJ, Jochle W. Treatment of transition phase mares with progesterone intra-vaginally and with deslorelinor hCG to assist ovulations. Journal of Equine Veterinary Science, (2002); 22 (2): 57-63.
18-Noden PA, Oxender WD, Hafs HD. The cycle of oestrus, ovulation and plasma levels hor¬ mones in the mare. /. Reprod. Fert., Suppl. (1975); 23, 189- 192.
Published
2018-06-28
How to Cite
K.M, Karam et al. A new approach for estrus induction in Arabian mares suffering from ovarian inactivity. Al-Qadisiyah Journal of Veterinary Medicine Sciences, [S.l.], v. 17, n. 1, p. 142-146, june 2018. ISSN 2313-4429. Available at: <http://qu.edu.iq/journalvm/index.php/vm_journal/article/view/490>. Date accessed: 10 dec. 2018. doi: https://doi.org/10.29079/vol17iss1art490.