Toxic pathological effect of mitomycin-c on male reproductive organs in rat

  • Hala Abbas Naji Department of pathology and poultery diseases/College of Veterinary Medicine/ University of AL-Qadisiyah
  • Khalil Gazar Chelab Department of pathology and poultery diseases/College of Veterinary Medicine/ University of AL-Qadisiyah
  • Atiaf Ghanim Rhyaf Department of pathology and poultry diseases, College of Veterinary Medicine/ University of AL-Qadisiyah
DOI: https://doi.org/10.29079/vol17iss2art517

Abstract

The aim of present study was detected the effect of Mitomycin-c on the histological structure on male reproductive organ. In this study used (24) healthy adult male rats were divided into three groups, each group includes (8) animals: the 1st toxic group was injected (6.5mg/kg) of Mitomycin-c subcutaneous (s/c) once daily, the 2ed therapeutic or low dose group was injected (3.25 mg/kg) of Mitomycin-c subcutaneous (s/c) once daily, while the 3rd control group was injected physiological normal saline (0.9% NaCl2). The experimental period was (10) weeks. Animal, which were treated with Mitomycin-c along the period of experiment showed, sever pathological changes, particularly in testes and epididymis.

References

1-Carter SK, Livingston RB. Drugs available to treat cancer. In Carter, S.K.:Principles of cancer treatment. McGraw, Hill.; New York. (1981).
2-Theilin GH. Chemotherapy. In Theilen GH, Madewell BR. Veterinary Cancer Medicine. 2 nd , Ed., Lea and Febiger, Philadelphia, (1987);pp 1315- 1318.
3-Boulton AJM, Carpenter JR, Clark B, Dascombe MJ, De akin JFM, Dive C, Duxbury AJ, Foster RW. Antiparasitic Chemotherap y. In Basic pharmacology. 4 th Ed., Oxford Auckland Boston and ohannesburg Melbourne, New Delhi, (1996);pp 269-274.
4-Danshiitsoodol N, de Pinho CA, Matoba Y, Kumagai T, Sugiyama M. "The mitomycin C (MMC)-binding protein from MMC-producing microorganisms protects from the lethal effect of bleomycin: crystallographic analysis to elucidate the binding mode of the antibiotic to the protein". J Molec Biol (2006);360 (2): 398–408.
5-Tomasz Maria. "Mitomycin C: small, fast and deadly (but very selective)." Chemistry and Biology (1995);2 (9): 575–579.
6-Renault J, Baron M, Mailliet P et al. Heterocyclic quinones 2. Quinoxaline-5,6-(and 5-8)-diones - Potential antitumoral agents. Eur. J. Med. Chem. (1981);16, 6, 545–550.
7-Hata TY Sano, R Sugawara, A Matsumae, K Kanamori, T Shima , T Hoshi. Mitomycin anew antibiotic from streptomyces. j antibiotic. (1956);9,141-146.
8-Wakaki SH Marumo, K Tomioka, G Shimizu, E Kato, H Kamado, S Kuda, Y Fujimoto. Isolation of new fractions of antitumor mitomycins .Antibiotic chemother. (1958);8,228-240.
9-Luna LG. Manual of Histologic staining methods of the armed forces. Institute of Pathology. 3 rd ,Ed., McGraw-Hill Book Company, N.Y., Toronto, London, Sydney (1968);pp.12-31.
10-Chemwatch Material Safety Data Sheet.( 2011). TCH 32647 C293LP Version No:6.1.1.1P:14 -19. www.biochem.uci.edu/.../MSDS/Mitomycin%20C%20 .
11-Van VJ , deRooij DG,Wensing CJ, Bootsma AL.Protection of spermatogenesis against cytotoxic effects of two chemotherapeutic drugs by temporary testicular blood flow interruption in the arm . Sep.Oct, (1993);25(5):251-6.
12-Xiao Y, Tates AD. Increased frequencies of micronuclei in early spermatids of rats following exposure of young primary spermatocytes to acrylamide. Sep.(1994);309(2):245-53.
13-Soichi N, Chisato M. Detection of mitomycin c induced testicular toxicity by micronucleus in mice. Vol.(2), No.(2), (2003); 69-73.del:10.1046/j.1445-5781. 2003.00023.x.
14-Nakagawa S,Nakamura N, Fujioka M , Moric. Spermatogenic cell apoptosis induced by mitomycin c in the mouse testis Toxicol Appl pharmacol .Dec. (1997);147(2):204-13.
15-Sjoblom T, Parvinen M, Landetie J. Stage specific DNA synthesis of rat spermatogenesis an indicator of genotoxic effect of vinblastine ,mitomycin-c &ionizing radiation on rat spermatogenia & spermatocytes .Mutat Res .Oct.(1995);331(2):181-90.
16-Drews Jurgen. Drug discovery :Ahitorical perspective )Science (2000);287:1960-1964.
17-Ritter JM, Lewis LD, Mant TGK. Chemotherapy. A textbook of clinical pharmacology. 4th Ed., Hodder Headline Group, London;(1999);pp:555-558.
Published
2018-12-30
Issue
Vol 17 No 2 (2018): Al-Qadisiyah Journal of Veterinary Medicine Sciences (QJVMS) 6th (1st international) Scientific Conference 27-28 Sep. 2017
Section
Research Article

Copyright (c) 2018 Al-Qadisiyah Journal of Veterinary Medicine Sciences

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Copyright: All manuscripts published by Al-Qadisiyah Journal of Veterinary Medicine Sciences are under the terms of the Creative Commons Attribution License. This permits anyone to copy, distribute, transmit and adapt the work provided the original work and source is appropriately cited. Copyright on any open access article published by Al-Qadisiyah Journal of Veterinary Medicine Sciences is retained by the author(s).

Note: The journal is not allow any scientific misconduct in any published research article. Any article exceeds the permissible ratio of plagiarism is rejected. However, as a publisher will follow strict scientific guidelines and advice to retract or erratum of any article at any time if found scientific misconduct or errors in any articles. Authors are responsible for obtaining permission from copyright holders for reproducing any illustrations, tables, figures or lengthy quotations previously published elsewhere.