ABSTRACT : The paper describes a field survey performed to determine the prevalence of hydatid cyst disease in cattle at slaughters in Al-Diwanyah abattoir. The study lasted for five months from November 2017 to April 2018. During the study, 200 cattle were examined. The study included 15 samples were collected from fluids and the germinal layer. The isolation and identification of E. granulosus were done using microscopic visualization followed by confirmation using a polymerase chain reaction (PCR) technique targeting the antigen B (AgB2) gene. The results indicate that(7.5%) of cattle were infected. The livers of cattle demonstrated a higher incidence of hydatid cysts than the lungs, with 6.66% oflivers and 20% of lung being infected, respectively. Also, the results of the study showed that the sex of the slaughtered animals has non-significant effect on the distribution of HC, as the rate of HC in males and females were close. The microscopic results revealed the presence of characteristic cysts. All E. granulosus isolates in cows appeared to be contained this gene show one distinct band (MW400 bp) when electrophoresed on agarose gel. The results of this study indicated that the PCR technique had a high specify in the detection of E. granulosus especially this species that encoded to AgB2 gene isolated from cowsin comparison to other routine diagnostic tests. ... قراءة المزيد

Aim of our study is investigation of the genetic relationships between Salmonella spp isolates which taken from human stool isolates and Salmonella spp isolates which taken from cattle meat. The total number of study isolates is one hundred (50 cattlebeef and 50human stools. Bacterial isolation and bacterial identification are used for testing the samples for culturing and examined by biochemical tests then detected by using 16S rRNA gene in PCR that designed by NCBI and make DNA sequencing for two positive isolates. Our results revealed that percentage of Salmonellaspp was 8/50(16%) which isolated from cattle beef and was 10/50(20%) which isolated from human stool. The PCR was demonstrated highly specific and sensitive. DNA sequencing of part of 16S rRNA was show highly similarity with NCBI-Blast data and the phylogenetic study was reveal genetic homology at (0.5 genetic change) between animals and human isolates and then the gene sequence deposited into NCBI-Genbank accession numbers accession numbers (MN523665.1) and (MG388337.1). In conclusion, our study is done for first-time in Iraq of the genetic correlating between Salmonellaspp which isolated from cattle meat and human stool. So, it is important to know the cattle meat is significant source for spreading infection between human and animals. ... قراءة المزيد