ABSTRACT
Mardenli O, Mohammad MS, Alolo AY. 2020. Effect of combination of follicle size, FSH and cysteamine on in vitro
production sheep embryos. JITV 25(3): 131-138. DOI: http://dx.doi.org/10.14334/jitv.v25i3.2517
The participatory relationship among the follicle size, follicle stimulating hormone (FSH), and cysteamine (antioxidant agent)
contribute to the production of embryos characterized by abundance and good quality. The aim of this study was to evaluate the
efficacy of FSH, cysteamine and follicle size on in vitro embryo production of Awassi sheep oocytes. Follicles sizes were
determined into two groups: small follicles (1-2 mm) and large follicles (> 2 mm). Oocytes were matured across two
increasingly shared levels of FSH and cysteamine: A (40 ng/ml + 50 μM) and B (60 ng/ml + 100 μM). Results of the bilateral
interaction showed significant differences across the follicle size (large follicles group) and the maturation treatment (B medium)
in the rates of fertilization (highest value: 67.51%; p= 0.02), cleavage (highest value: 65.41%; p= 0.01), 2-16 cell stage (lowest
value: 2.29%; p= 0.0001), blastocyst stage (highest value: 44.82%; p= 0.04), down to morula stage arrest (lowest value: 55.17%;
p= 0.04) and Type I embryos (highest value: 52.87%; p= 0.03). Likewise, matured oocytes of small follicles group (B medium)
attained the highest rate of morula stage (56.60%; p= 0.03). No significant differences were observed in Type II and Type III
embryos. In order to obtain high yields of good quality embryos, it is advised to add FSH and cysteamine with levels of 60 ng/ml
and 100 μM respectively to maturation medium of ovine oocytes obtained from follicles with a diameter > 2 mm.