Ahmed A.H. Abdelmonem / Egypt

 

 

 

 

 Curriculum Vitae

Ahmed A. Hussein Abdelmonem

Prof. of Biotechnology

Contact Information

Texas Tech University

College of Arts and Science

Department of Chemistry and Biochemistry

Box 41061 | Lubbock, Texas 79409-1061

Mobile Phone:          806-224-5567

European Commission expert candidature number: EX2014D224994

ahmed.abdelmonem@ttu.edu

University of Alexandria,

Department of Biotechnology,

Institute of Graduate Studies & Research,

163-Horreya Avenue, El-Shatby, P.O. Box 832,

Alexandria 21526, Egypt.

Tel (Home):   002-03-3316339

Tel (Work):     002-03-4295007

Mobile Phone:          002- 01227922071

Fax:    002-03-4285792

ahmed.hussein@alexu.edu.eg

unarc2000@hotmail.com

 Personal Information

Birth date: 24

Jan, 1968

Marital status: Married

Research Interest

 Microbiology and Molecular Biology, Medical Biotechnology

Structural/function of biological molecule using mass spectrometers

Glycobiology and vaccinology (Conjugate vaccine, bacterial

ghost and adjuvant preparation)

Diagnosis of cancer and metabolic diseases by Glycoproteomics based tools

Education

1990: B.Sc. in Microbiology, Faculty of Science, Alexandria University, Excellent Distinction, Egypt.

1994: M.Sc. in Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Egypt. Fellowship at University of Manchester Institute of Science and Technology (UMIST), UK.

Title: Isolation and characterization of bacterial mutant strains for enhancement of crude oil biodegradation

2001: Ph.D. in Microbiology,  Charles/Comenius University, Faculty of Science, Czechoslovakia.

Title: Coxiella burnetii and Chlamydial lipopolysaccharides: Studies on their chemical structures and biomedical applications.

Scientific collaborations

  1993: Research visitor, Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology, Manchester, United Kingdom. (3 months)

 1997: Research assistant, (WHO) Reference Center, Department of Rickettsiology and Chlamydiology, Institute of Virology, Czechoslovakia, (5-years)

  2001: Research visitor, University

of South of Paris, Institute of Biochemistry and Biophysics. CNRS, endotoxin group, Paris, France. (3-months)

 2001: Research

visitor, Unite des Rickettsies, CNRS UPRES-A 6020, Faculte de Medicine, Universite de la Mediterranee, Marseille, France. (1-month)

 2002: Research visitor, European Molecular Biology Center in

Hamburge

, Germany. (2-months)

2003: Post-doctor (Agency of Francophone Universities), University

of South of Paris, Institute of Biochemistry and Biophysics. CNRS, Endotoxin Group, Paris, France. (1-year)

 2005: Visiting Professor, University

of South of Paris, Institute of Biochemistry and Biophysics. CNRS, Endotoxin Group (Joint Research Project: France, Morrocco

and Egypt). Paris, France. (6-months)

2006: Visiting Professor, Slovak Academy of sciences, Institute of Virology, Slovakia. (3-months)

 2007: Post- doct

, National center for glycomics and glycoproteomics, Chemistry Department, Indiana University, USA. (2-years) 

 2009: Visiting Professor, International Center for Science and High Technology-United Nations Industrial Development Organization, ICS-UNIDO, Trieste, Italy.  (1-month)

 2009: Visiting professor, National center for glycomics and glycoproteomics, Indiana University, USA. (2-months)

 2010: Visiting professor, National center for glycomics and glycoproteomics, Indiana University,    USA. (2-months)

 2011: Visiting Professor, University

of South of Paris, Institute of Genetics and Microbiology,   France. (3-months)

2011-Now: Senior Research Associate (Research Professor), Texas Tech University, Texas, USA.   

 Professional Memberships

  • Water Environmental Federation, USA
  • Egyptian Society for Water Environmental Affaires
  • European Working Group of Rickettsiology
  • The African Association of Physiological Sciences
  • American Society of Chemistry
  • American Society of mass spectrometry
  • Human proteome organization
  • Committee of Scientific Research, Institute of Virology, Slovak Academy of Science, Slovakia,
  • Alliance of glycobiologists, National cancer Institute, USA

 

Employment History

1990-1994: Instructor

Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University.

1994-1997: Assistant Lecturer

Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University.

1997-2002: Research Assistant

WHO Reference Center, Czechoslovakia

2002-2009: Assistance Prof.

Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University.

2009-2013: Associate Prof.

Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University.

2014-Now: Full Professor

Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University.

Consultations

1990-Curent: Pharmaceutical sectors in Egypt.

Honors and Awards

2001: The best applied

research results, WHO Reference Center, Czechoslovakia.

2007: Alexandria University Award of scientific encouragement, Alexandria University, Egypt.

2013: Alexandria University Award of Biotechnology, Alexandria University, Egypt

Workshops

2003: New Era of Biotechnology in pharmaceutical industries, Mubarak City for Science and Technology, Alexandria, Egypt.

2004:

Bio vision

Alexandria. The New Life sciences: Ethics, Patents and the Poor. Organized in Partnership with the World Life Sciences Forum, Bibliotheca Alexandria, Alexandria, Egypt

2004: Center of Excellence Alexandria University meeting held at the Institute of Graduate Studies and Research, Alexandria, Egypt.

2004: Internal quality assurance system in higher education, Faculty of Agriculture, Cairo University, Egypt.

2004: Accreditation system in post-graduate studies, Alexandria University, Faculty of Nursing, Alexandria University, Egypt.

2005: Workshop on time

of flight mass spectrometry and its applications, Alexandria, Egypt.

2006: Workshop on aquaculture, Institute of Graduate Studies and Research, Alexandria, Egypt.

2006: Second environmental physics conference, organized by Egyptian nuclear physics association, Alexandria, Egypt.

2006: Workshop on scientific research, organized by Agence Universitaire de la Francophonie,   Bibliotheca Alexandrina, Alexandria, Egypt.

2006: First-environmental workshop, Alexandria University, Alexandria, Egypt.

2009: Science Parks, Korean-Egyptian scientific committee for scientific

affaires

, Mubarak City of scientific research, Alexandria Egypt.

2009: Biomedical Applications of Biodegradable Polymers, September 28-29, Alexandria, Egypt

2010: The International Workshop on Industrial Biotechnology. Under The Umbrella Of

The France-Egypt Year Of Science And Technology,  Egypt

2010: International winter school on `Research promotion, technology transfer and business creation` from November 22nd to 24th in Alexandria, Egypt.

2012: Workshop on mass spectrometry and its applications, Faculty of Medicine, Alex. University.

2012: Workshop on Chromatography and its applications‏, Institute of Graduate Studies and Research, Alexandria University

2012: The Establishment of a Joint Research  Facility between UNU-INWEH and the Alexandria University, 12-13 May, Alexandria, Egypt

2014: Safety in lab, Texas Tech University, Department of Chemistry and Biochemistry, Texas, USA

2014: International 2th workshop on Molecular Therapeutics, in partnership with Cultural French Center in Cairo. 15-18th December. Invited speaker

2015: International 2th workshop on Molecular Therapeutics, in partnership with Cultural French Center in Cairo. 11-18th September. Invited speaker

Projects

1992-1997: MED-CAMPUS Project, European community.

1994-1997: In plant modification and improvement of handling processing to reduce wastes at Al- Amerya

Petroleum Refining Co. USAID.

1997-2002: Studies on the structural/function relationship of

lipopolysaccharide

of Coxiella burnetii, Slovak Academy of Sciences and the Ministry of Education of Czechoslovakia.

2003-2005: Rhizobium modulating Acacia

.Agency of Francophone Universities. Inter-university project among Egypt,

France and Morocco.

2004-2006: Biotechnology Training Center Project, supported by the Higher Education

Department ,

Ministry of Higher Education of  Egypt.

2007-2010: Glycomics and glycoproteomics analysis of biological materials, NIH, Indiana University, USA.

2009-2010: Quality Assurance and Accreditation Program. Egypt.

2009-2012: Accreditation and Quality Assurance Program, Egypt.

2010-2015: ISIS Euro-Mediterranean Master in Neuroscience and biotechnology, TEMPUS, FP7 Project.

2012-Now:  Proteome Lab, Capacity building program, Faculty of medicine, Alexandria University

2012-Now: Diagnosis of breast Cancer, STDF

2014-Now:

Alexprep

. Project in collaboration with Faculty of Medicine, Department of Microbiology and immunology, Alexandria, Egypt, Diagnosis of Mycobacterium tuberculosis
2013-Now: CPRIT Grant For Research Into How Breast Cancer Cells Metastasize To The Brain, Texas Tech University, Texas, USA

2015-Now: Glycomics platform, NIH, Texas Tech University, Texas, USA

Activities

2000-2002: Executive chairman of the Egyptian Student Union in Czechoslovakia, Embassy of the Arab Republic of Egypt, Vienna, Austria.

2002: Inviting Prof. R. Toman (UN and UNIDO expert), as a visiting professor

to Alexandria and Ain Shams Universities.

2005: Inviting Prof. Martine CAROFF (

University

of South of Paris, Institute of Biochemistry, France) and Prof. Abd El-Karim

Falali

(University of Mohammed V, Rabat, Morocco).

2000-Current:  Peer-Review of the international journal of environmental sciences, CATRINA.

2009-Current: Peer-Review of Electrophoresis.

2012: Coordinator of Grant Initiative Technology Transfer Center, Alexandria University.
Research and professional experience

 Studies of the physicochemical characterization of the endotoxins

  1. Preparation of antigens from pathogenic microorganisms
  2. Preparation of vaccine and immunodiagnostic reagents
  3. Preparation of adjuvants
  4. Studies of the immunological role of monosaccharides

6.    Studies of the O-polysaccharide antigen as an immunodiagnostic reagent.

7.    Studies of the bacterial lipid A: easy and rapid method for mass spectrometric characterization.

8.    Studies of the immunization trials against Klebsiella pneumoniae.

9.    Studies of the quantitative serum glycomics of esophageal adenocarcinoma and other esophageal disease onsets.

10. Studies of the assigning N-glycosylation sites of glycoproteins using LC/MSMS in conjunction with endo-M/exoglycosidase mixture.

  1. Studies of the comparative glycomic profiling in esophageal adenocarcinoma.
  2. Studies of the microchip electrophoresis of N-glycans on serpentine separation channels with asymmetrically tapered turns.
  3. Studies of the enhanced sensitivity of LC-MS analysis of permethylated N-glycans through online purification.
  4. Studies of the n-linked Glycan Structures and Their Expressions Change in the Blood Sera of Ovarian Cancer Patients.
  5. Production of valuable compounds like bacteriocin and siderophore from bacteria
  6. Natural anti-cancer agents from bacteria and fungi

Teaching Experience

Department of Biotechnology

 Diploma students:

  • Microbiology and fermentation technology; Biochemical analysis in bioscience (I/II); Applications of biotechnology; Microbial Biotechnology; Bases of biotechnology; Bases of  molecular biology; Methods in microbiology

M.Sc. students:

  • Applications of biotechnology; Microbial Biotechnology; Bases of biotechnology; Bases of  molecular biology; Biochemical analysis in bioscience (I/II); Methods in microbiology; Macromolecules; Advanced biochemistry; Methods in molecular biology; Medical waste, hazardous and management; Molecular diagnosis of diseases; Enzymes and their applications

Ph.D. students:

  • Molecular biology techniques; Advanced immunology; Advanced biochemistry; Biofertilizers; Biopigments; Proteomics and genomics; Antioxidants; Advanced enzymology and its applications

 Department of Environmental Studies

  • Applied microbiology; Microbiology of stressed environments; New advancements in genetic; control of microorganisms

 Faculty of Science, Alexandria University

  • Proteomics and genomics

Number of supervised degrees

  • 25 students: Diploma (Awarded)
  • 25 students: Sc.   (Awarded and 10 undergoing)
  • 15 students: D. (Awarded and 5 undergoing)

Post-experience training courses

1992: Biodegradation of crude oil in aquatic system

2003: Molecular diagnosis of diseases

2004: Protein analysis

2004: Biotechnology in molecular diagnosis of diseases

2004-2006:   Biotechnology Training Center Project, Higher Education

Department ,

Ministry of Higher Education of

Egypt ,

Instrumentations, Applications of biotechnology, Basics of protein analysis, Bioinformatics and Molecular diagnosis of the diseases

2007: Bioinformatics, Institute of Graduate Studies and Research

2009: Biomedical applications of Biopolymers in cooperation of Royal Swedish Institute.

2009-2010: Environmental health Risks

References

Dr. Rudolf Toman: Institute of Virology, Czechoslovakia

Prof. Milos Novotny, Indiana University, Chemistry Department, Indiana, USA

Dr. Helena Soini,  Indiana University, Chemistry Department, Indiana, USA

Dr. Martine Caroff: CNRS, Paris, France

Dr. Ludovit Skultety: University of Rice, Houston, Texas, USA.

Prof. Yahia Mechref: Texas Technical University, Texas, USA.   

  Conferences

  1. Ftacek, P., Skultety, L., Hussein, A. & Toman, R. (1998) Compositional changes in the outer membrane glycose polymer of Coxiella burnetii during the phase variation. In: Book of Abstracts, 21th Congress of the Czechoslovak Society for Microbiology, 6-10 September, Hradec Kralove, Czech Republic, p.218.
  2. Hussein, A., Kovacova, E., Ftacek, P. & Toman, R. (1999) Evaluation of Coxiella burnetii lipopolysaccharide components as the immunodiagnostic reagents. In: Book of Abstracts, EUWOG-ASR joint meeting, 14-16 June, Marseille, France, p. 17.

 

  1. Hussein, A., Patoprsty‎, V., & Toman, R. (1999) Compositional and structural analyses of the lipopolysaccharide of Chlamydia psittaci PK 5082. In: Book of Abstracts, International School for Molecular Biology and Microbiology, 13-18 November, Bratislava, Slovakia, p. 32.
  2. Hussein, A., Kovacova, E., & Toman, R. (2000) O-polysaccharid von Coxiella burnetii als mِgliches antigen für die Diagnose des Q-fiebers. In : Book of Abstracts,  5. Internationale Tagung, Infektionskrankheiten in den Alpenlandern, 23-25 Marz, Seis am Schlern (BZ), Italien, p. 82-83.

 

  1. Hussein, A., Patoprsty‎, V., & Toman R.  (2000) Eigenschaften des lipopolysaccharides von Chlamydia psittaci-Stamm PK 5082. In: Book of Abstracts, 5. Internationale Tagung, Infektionskrankheiten in den Alpenlandern, 23-25 Marz, Seis am Schlern (BZ), Italien, p. 108.

 

  1. Toman, R., Hussein, A. &  Skultety, L. (2000) Structural features of lipopolysaccharides from two Chlamydia psittaci strains PK 5082 and 6BC. In: Book of Abstracts, 9th International Congress on Infectious Diseases, 10-13 April, Buenos Aires, Argentina, p. 256 (95.237).

 

  1. Hussein, A., Kovacova, E. & Toman, R. (2000) Studies on application of Coxiella burnetii O-antigen in Q fever diagnosis. 6th Conference of the International Endotoxin Society, 24-27 August, Paris, France. Abstract in: J. Endotoxin Research6, 2000, 150,  P105.

 

  1. Toman, R., Ftacek, P., Hussein, A. &  Palkovic, P.  (2000) Structural features of lipopolysaccharides from virulent and low-virulent phases of Coxiella burnetii, the etiological agent of Q-fever. In: Book of Abstracts, 20th International Carbohydrate Symposium, 27 August- 1 September, Hamburg, Germany, p. 75, A-216.

 

  1. Toman, R., Hussein A., Ftacek, P.  & Palkovic P.  Lipopolysaccharides from virulent and low-virulent phases of Coxiella burnetii, the etiological agent of Q fever and Q fever endocarditis. In: Book of Abstracts, 11th European Carbohydrate Symposium, 2-7 September, Lisboa, Portugal, p. 19., 2001.

 

  1. Hussein, A., Palkovic, P. & Toman, R. Chlamydial lipopolysaccharides: Chemical composition and structure.  In: Book of Abstracts, Structure and Stability of Biomacromolecules, 12-14 September, Kosice, Slovakia, pp. 53-54, 2001.

 

  1. Hussein, A., Bystrick‎, S., Kovacova E. & Toman R.   Preparation, characterization, and immunological properties of polysaccharide-protein conjugate as a vaccine against Q fever. In: Book of Abstracts, Structure and Stability of Biomacromolecules, 12-14 September, Kosice, Slovakia, pp. 97-98, 2001.

 

  1. Slaba, K., Hussein, A., Palkovic, P. & Toman R. Studies on immunological role of virenose and dihydrohydroxystreptose present in the Coxiella burnetii phase I lipopolysaccharide.  In: Book of Abstracts, International Conference on rickettsiae and Rickettsial diseases joint with ASR Conference.4th -7th September, Medical faculty, Korytkova 2, Ljubljana, Slovenia, 2002
  1. Toman, R., Hussein, A., Palkovic, P. & Ftacek, P. Structural properties of lipopolysacharides from Coxiella burnetii Henzerling and S.   In: Book of Abstracts, International Conference on rickettsiae and Rickettsial diseases joint with ASR Conference.4th -7th September, Medical faculty, Korytkova 2, Ljubljana, Slovenia, 2002.
  1. Hussein, A, Martine, C. & Toman, R.  Lipid A of Coxiella burnetii strain Nine Mile in virulent phase I and low-virulent phase II: Chemical composition and structure. In: Book of Abstracts, 12th European Carbohydrate Symposium, Grenoble, France, July 6-11, 2003.
  2. Toman R., Hussein A., Caroff M. & Skultety L. Lipid A is the endotoxic principle of the Coxiella burnetii lipopolysaccharide; Estimation of its composition and structural features. In the book of abstracts of 55th Congress of Chemical Society, Kosice, Slovakia, September 8-12, 2003.
  3. El-Hamidi Asmaa, Tirsoaga Alina, Hussein Ahmed, Senechal Magalie et Caroff Martine.  Nouvelles methods d`analyse des lipopolysaccharides bacteriens a partir de microquantites de cellules.. In: Book of abstracts, Congres Groupe Francais de Glucides. 25-28 Mai, Dourdan, ILE de France, France, 2004.
  4. Asmaa El-Hamidi, Alina Tirsoaga, Ahmed Hussein, Abdel Karim Filali-Maltouf, Fatiha Bhrada et Martine Caroff. Caracterisation d’endotoxines bacteriennes par MALDI a partir de microquantites de cellules. In the book of abstracts, 21 th  French Mass Spectrometry Meeting, 14 th    -17 th  September, Strasbourg, France, 2004. 
  5. Caroff M., Tirsoaga A;, El-Hamidi A., Hussein A., Novikov A., Adib-Conquy M., Werts C. & Cavallion J-M. New methods for the extraction, purification and quick extraction of bacterial endotoxins. In the book of Abstracts, 8 th Biennial Conference of the International Endotoxin Society, Kyoto, Japan, 15-18 Nov. 2004.
  6. Hussein A., Novikov A., Perry M., and Caroff  M. Structural characterization of lipid A of Bordetella holmesii by MALDI-TOF mass spectrometer. In the book of abstracts, 17th International Conference of Mass Spectrometery, Prague, Czech Republic, 28th August-1st of September, 2006.
  7. Hussein, A., Novikov, A., Perry, M., and Caroff, M. Lipid A of  Bordetella holmesii as a linkage between B. brochiseptica and B. trimatum. 8th International Symposium: Saga of the Genus Bordetella, 1906-2006. Pasteur  Institute, 7-10 Nov. France, 2006.
  8. Hussein, A., Danial, K., Mechref, Y., and Novotny, M. Isolation and characterization of the exopolysaccharide required for biofilm formation in Bacillus subtilis. 56th  conference of the American Society of mass spectrometry, Dever, Colorado, 1-5 June, USA, 2008.
  9. Mechref, Y., Novotny, M., Alley, W., Kyselova, Z., Madera, M., Mann, B., Klouckova, I., Goetz, J., Hussein, A. and Kang, P. Development of glycomics and glycoproteomics tools for cancer biomarker discovery. American Chemical Society, USA, 2008

 

  1. Novotny, M., Mechref, Y., Hussein, A., Saleh, M., Alley, W., Kang, P., Madera, M., Goetz, J., Maldi-Tof/MS and CE-LIF glycomics mapping in cancer diagnosis and prognosis measurements. MSB, 1-5 February, Boston, USA, 2009.
  2. Zane Hammoud, Yehia Mechref, Ahmed Hussein, Slavka Bekesova, Min Zhang, Kenneth Kesler, Robert Hickey, Milos Novotny.. 89th Annual Meeting of the American Association of Thoracic surgery, May 9-13, 2009. Boston, Massachusetts, USA.
  3. Ahmed Hussein, Saad Salah, Medhat Haroun and Hesham El-Masry. Alginates of the Egyptian marine brown algae display anti-tumorigenic activity against Agrobacterium tumefaciens. The International Conference on: “Current Trends in Medicinal Plant Research and Microbiological Applications”(October 27-29/ 2010), Alexandria, Egypt.   (Oral representation)
  4. Laila H. El-sayed, El-Ashray E. H., Haroun M.A., Hussein   A. A., Ahmad T. A. Screening of noval epitopes in Klebsiella pneumonia, as target for vaccine design in Alexandrian population. Frontiers in immunology research international conference. July, 1-4, 2011, in Split Croatia. (Oral representation)
  1. Malika Alili, Zaghloul TI and Ahmed Hussein. Purification and characterization of alkaline protease with feather degradation from the recombinant strain of Bacillus subtilis dnaC 30 temperature sensitive mutant. V International Conference on Environmental, Industrial and Applied Microbiology, Madrid, Spain, 2-4 October 2013. (Oral representation)
  2. Malika Alili, Zaghloul TI and Ahmed Hussein. Purification and characterization of alkaline protease with feather degradation from the recombinant strain of Bacillus subtilis dnaC 30 temperature sensitive mutant. 2th Biotechnology congress, 2013. (Oral representation)
  3. Malika Alili, Zaghloul TI and Ahmed Hussein. Biodegradation of native feather keratin by the recombinant strain of Bacillus subtilis dnaC 30 temperature sensitive mutant. IV International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2011) 14-16 September, 2011, Torremolinos (Spain) (Oral representation)
  4. Malika Alili, Zaghloul TI and Ahmed Hussein. Enhancing expression and stability of the cloned alkaline protease (aprE) gene in Bacillus subtilis dnaC30 temperature sensitive mutant.The Second International Conference on Biotechnology, 26-29 April 2010, Oran, Algeria (Oral epresentation)
  1. Hanan Mostapha, T. I. Zaghloul, Hassan F., Ahmed Hussein. Production and Optimization of Surfactin from two Bacillus strains isolated from environmental waste. BioVision, April, 2012, Alexandria, Egypt.
  1. Yehia Mechref , Yunli Hu , Janie L. De Santos-Garcia , Ahmed Hussein , Shiyue Zhou. Probing the Glycomic Signature of Brain Metastasis in Breast Cancer. The 36th International Symposium on Capillary Chromatography. May 27 – June 1, 2012 in the Congress Centre, Riva del Garda, Italy. (Oral representation)
  2. Ahmed Hussein, Manal Ali, Shwekar Abdel-Salam, Ezzat Ali, Pasent Moez. Detection of Helicobacter pylori lipopolysaccharide-fucosyltransferases among gastroduidenal patients. First conference of advancements in botany and micobiological technologies, July, 2012, Alexandria, Egypt. (Oral representation)

 

  1. Hanan Mostapha, T. I. Zaghloul, Hassan F., Ahmed Hussein. “Optimization and kinetic study of a Bacillus surfactin and its possible application for soil washing“. BioVision, March, 2014, Alexandria, Egypt.
  2. Ahmed Hussein, Daniel Kearns, Yehia Mechref, Milos Novotny. Exopolymeric materials from Bacillus subtilis biofilm: Easy and rapid method for mass spectrometric characterization. The 14th Arab International Conference of Material Science, 1-3 December 2013, Alexandria, Egypt
  3. Ahmed Hussein,  Shrine Sameh,  Amal AlKamal,  Amal Youssef,  Magda Nasr. Isolation and Characterization of Phenazine from  Pseudomonas aeruginosa and Evaluation of  Its Redox Activity after Derivatization. The 11th International Symposium on Biocontrol and Biotechnology, October 1-4, 2013, Alexandria – Egypt. (Oral representation)
  4. Separation of permethylated glycan isolated by bacterial PNGase-F. PITTCON, USA, 2014. (Oral representation)
  5. Shwekar Abdel-Salam , Ahmed Hussein, Manal Ali, , Ezzat Ali, Pasent Moez. The Prevalence of Cag A and Vac A Genotypes among Helicobacter pylori Infected Egyptian Patients.  American Society of Microbiology Conference, Boston, May, 2014
  6. Amira Embaby, Ramy Meleka, Ahmed Hussein, Amel El-Kamel. A noval approach towards biosynthesis of chitosan-oligosaccharides by a endochitosanase producing Aspergillus flavus.  TWAS Symposium on Industrial Biotechnology, October 15th-18th, 2014 Beijin, China
  7. Lucas Veillon, Ahmed Hussein, Yehia Mechref, Microscale Chemical Release of O-linked Oligosaccharides by RAIDR. 65th ASMS Conference, June 4-8, 2017, Indianapolis, Indiana, USA

 

 

Articles:

  1. Hussein, A., Patoprsty‎, V. & Toman, R. (1999) Chlamydia psittaci lipopolysaccharide. A reinvestigation of its chemical composition and structure. Acta Virol. 43, 381-386.
  2. Hussein, A., Kovasova, E. & Toman, R. (1999) Application of O-polysaccharide antigen of Coxiella burnetii in diagnosis of Q-fever. Bull.  Czechoslovak  Soc. Microbiol. 23, 81-83.
  3. Ftacek, P., Hussein, A. & Toman, R. (1999) Phase variation of Coxiella burnetii. Bull. Czechoslovak  Soc. Microbiol. 23, 80-81.
  4. Hussein, A., Skultety, L.  & Toman, R. (2001) Structural analyses of the lipopolysaccharides from Chlamydophila psittaci strain 6BC and Chlamydophila pneumoniae strain Kajaani 6.     Carbohydr. Res. 336, 213-223.
  5. Hussein, A., Kovacova, E. & Toman R. (2001) Isolation and evaluation of  Coxiella burnetii O-polysaccharide antigen as an immunodiagnostic reagent.  Acta Virol. 45, 173-180.
  6. Slaba, K., Hussein, A., Palkovic, P. & Toman R. (2003) Studies on immunological role of virenose and dihydrohydroxystreptose present in the Coxiella burnetii phase I lipopolysaccharide.  Ann N Y Acad Sci. 990:505-9.
  7. Toman, R., Hussein, A., Palkovic, P. & Ftacek, P. (2003) Structural properties of lipopolysacharides from Coxiella burnetii Henzerling and S.  Ann N Y Acad Sci. 990:563-7.
  8. Toman, R., Hussein, A., Slaba, K. & Skultety, L. Further structural characteristics of the lipopolysaccharide from Coxiella burnetii strain Nine Mile in virulent phase II. Acta Virol. 47, 57-59, 2003.
  9. Toman R., Hussein A., Caroff M. & Skultety L. Lipid A is the endotoxic principle of the C. burnetii lipopolysaccharide. Chemicke Listy, 97:8,751-752, 2003.
  10. Physicochemical characterization of the endotoxins from Coxiella burnetii strain Priscilla in relation to their bioactivities. BMC Biochem. 12;5(1): 1-11,  2004.
  11. Caroff, M., Tirsoaga, M., El-Hamidi, A., Hussein, A., Novikov, A., Adib-Conquy, M. and Cavaillon, J.M. New methods for the extraction, purification and quick extraction of  bacterial endotoxins. Journal of  Endotoxin  Research , Vol. 10, Number 5, 79-80, 2004.
  12. Microextraction of bacterial lipid A: easy and rapid method for mass spectrometric characterization. J Lipid Res. Aug; 46(8):1773-8, 2005. 
  13. Hussein, A. Flash isolation method for lipopolysaccharide from some Gram-negative bacteria.  Alexandria journal of Pharmaceutical Sciences, vol. 19 (2), Sept., 161-166, 2005.
  14. Yehia, M., Hussein, A., Bekesova, S., Pungpapong, V., Zhang, M., Dobrolecki, R., Hickey, J., Hammoud, Z. and Novotny, M. Quantitative serum glycomics of esophageal adenocarcinoma, and other esophageal disease onsets. J. Proteome Res. 2009 Jun;8(6):2656-66.
  1. Hammoud ZT, Mechref Y, Hussein A, Bekesova S, Zhang M, Kesler KA, Novotny MV. J Thorac Cardiovasc Surg. 2010 May; 139(5):1216-23.

 

  1. Zaneer M. Segu, Ahmed Hussein, Milos V. Novotny and Yehia Mechref. Assigning N-Glycosylation Sites of Glycoproteins using LC/MSMS in Conjunction with Endo-M/Exoglycosidase.  J. Proteome Res. 2010 Jun;8(6):2656-66.

 

  1. Zhuang, Zexi; Mitra, Indranil; Ahmed, Hussein; Novotny, Milos V; Mechref, Yehia Salem; Jacobson, Stephen C. “Microchip Electrophoresis of N-Glycans on Serpentine Separation Channels with Asymmetrically Tapered Turns”. Electrophoresis,  2011 Jan;32(2):246-53.

 

  1. Ahmed Hussein, Saad Salah, Medhat Haroun and Hesham El-Masry. Alginates of the Egyptian marine brown algae display anti-tumorigenic activity against Agrobacterium tumefaciens,  Egyptian Journal of Botany, pp. 68-84, volume 27-29 October 2010.
  2. Janie L. Desantos-Garcia, Sarah I. Khalil, Ahmed Hussein, Yunli Hu and Yehia Mechref. Enhanced sensitivity of LC-MS analysis of permethylated n-glycans through online purification.   Electrophoresis, 2011 Dec;32(24):3516-25.
  1. Alley WR, Vasseur JA, Goetz JA, Svoboda M, Mann BF, Matei DE, Menning N, Hussein A, Mechref Y, Novotny MV. J Proteome Res. 2012 Apr 6;11(4):2282-300.
  2. Ahmad TA, El-Sayed LH, Haroun M, Hussein AA, El Ashry ES. Vaccine. 2011 Nov 17.
  3. Screening of Novel Epitopes in Klebsiella pneumoniae, as Target for Vaccine Design
    by Laila H. El-Sayed, Medhat A. Haroun, Ahmad A. Hussein, El Sayed H. El Ashry, Tarek A. Ahmad. International Journal of Immunological Studies, volume 1(4)-Jan. 1, 2012  
  4. Identifying Cancer Biomarkers through Mass Spectrometry Based Glycomics. Mechref Y, Hu Y, Garcia A, Hussein A. Electrophoresis. 2012 Jul;33(12):1755-67
  5. Development of a new trend conjugate vaccine for the prevention of Klebsiella pneumoniae. Tarek A. Ahmad, Medhat Haroun, Ahmed A. Hussein, El Sayed H. El Ashry, Laila H. El-Sayed | Infectious Disease Reports: 2012-07-23 17:21:48
  6. Defining putative glycan cancer biomarker by MS. Bioanalysis. 2012 Oct;4(20):2457-69.
  1. Quantitative glycomic strategies.  Mol Cell Proteomics. 2013 Apr;12(4):874-84.

 

  1. Evaluating the Surface antigen of the Salmonella typhimurium ATCC 14028 Ghosts (STGs) prepared by “SLRP”.  The Scientific World journal.  Volume 2014, Article ID 840863, 6 pages

 

  1. Ahmed Hussein, Manal Ali, Shwekar Abdel-Salam, Ezzat Ali, Pasent Moez. Detection of Helicobacter pylori lipopolysaccharide-fucosyltransferases among gastroduidenal patients. Journal of Molecular Diagnosis and Vaccines, 11, 2013 Page: 09-17

 

  1. Amira Embaby, Yasmine Kamel, Ahmed Hussein and Heba Marey. A Sequential Statistical Approach towards an Optimized Production of a Broad Spectrum Bacteriocin Substance from a Soil Bacterium Bacillus sp. YAS 1 Strain. Accepted to The Scientific World Journal, 2014;2014:396304. doi: 10.1155/2014/396304.

 

  1. Rafik Abbas, Ahmed Hefnawy, Ahmed Hussein, Nashwa Ashraf. Enzymatically modified chitosan by Streptomyces lividans as a green inhibitor for steel corrosion in acidic medium. Accepted to International Journal of Chemistry and Applied Biological Sciences. Volume 1, 1-8, 2015

 

 

  1. CdTe Quantum Dots as a Novel Biosensor for Serratia marcescens and Lipopolysaccharide, Accepted Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, May, 2015

 

  1. Bioethanol Production from Shrimp Shell Waste Using Marine Alcaligenes feacalis MS7 and Candida albicans ATCC 14053. H. Ibrahim, A. Hussein, and M. Abd El-Waness. Volume 3, issue 3, 2014

 

 

  1. Song E., Hussein A., Mechref Y. HIV glycoproteins heterogeneity. Journal of Virology, Submitted

 

  1. Song, Y. Hu, A. Hussein, C-Y. Yu, H. Tang, Y. Mechref “Comprehensive Characterization of the Glycosylation Site of Human PSA Prompted by Missense Mutation using LC-MS/MS” J Proteome Res. 2015 Jul 2;14(7):2872-83.

 

  1. Gaye, M. M.; Ding, T.; Shion, H.; Hussein, A.; Hu, Y.; Zhou, S.; Hammoud, Z. T.; Lavine, B. K.; Mechref, Y.; Gebler, J. C.; Clemmer, D. E. Delineation of Disease Phenotypes Associated with Esophageal Adenocarcinoma by MALDI-IMS-MS Analysis of Serum N-linked Glycans , submitted.

 

 

  1. Hussein, S. Zhou, Y. Hu and Y. Mechref “Multiplexed Comparative Glycomic Mapping (MC-GlycoMAP) for Quantitative Glycomics“, Chem., submitted.

 

  1. Hussein, Y. Hu, Shiyue Zhou and Y. Mechref “Glycomic Mapping of Blood Serum of Esophagus Diseases Facilitated by Endo M” J. Proteome Res., submitted.

 

  1. Hussein, Y. Hu, Shiyue Zhou and Y. Mechref. New method of O-glycan preparation, In preparation, J. Proteome Res., submitted.

 

 

39.  Amira M. Embaby · Yasmin Heshmat · Ahmed Hussein. Unusual non-fluorescent broad spectrum siderophore activity (SID EGYII) by Pseudomonas aeruginosa strain EGYII DSM 101801 and a new insight towards simple siderophore bioassay. Applied Microbiology and Biotechnology Express, 6 (26) 2016.

 

  1. Hussein, A., Caroff, M. & Toman, R. Structure of Lipid A from Coxiella burnetii strain Nine Mile.    Rapid Commun. Mass Spectrom.  submitted
  1. EmbabyAM, Melika RR, Hussein A, El-Kamel AH, Marey HS . A Novel Non-Cumbersome Approach Towards Biosynthesis of Pectic-Oligosaccharides by Non-Aflatoxigenic Aspergillus sp. Section Flavi Strain EGY1 DSM 101520 through Citrus Pectin Fermentation. PLoS One. 2016 Dec 19;11(12 ).
  2. EmbabyAM, Saeed H, Hussein SHG10 keratinolytic alkaline protease from Bacillus licheniformis SHG10 DSM 28096: Robust stability and unusual non-cumbersome purification. J Basic Microbiol. 2016 Dec;56(12):1317-1330
  3. Novel Impedimetric and voltammetric sensors for tetracycline based on polyaniline modified electrode. Sensor Letters 14(5):484-489. May 2016

Review articles

 

  1. Ahmad TA, El-Sayed LH, Haroun M, Hussein AA, El Ashry ES. Vaccine. 2011 Nov 17

 

  1. Identifying Cancer Biomarkers through Mass Spectrometry Based Glycomics. Mechref Y, Hu Y, Garcia A, Hussein A. Electrophoresis. 2012 Jul;33(12):1755-67

 

  1. Defining putative glycan cancer biomarker by MS. 2012 Oct;4(20):2457-69.

 

  1. Quantitative glycomic strategies. Mol Cell Proteomics. 2013 Apr;12(4):874-84.

 

Patents

 

Vaccine

against Septicaemia Septivac vaccine,   Grant Initiative Technology Transfer Center, Alexandria University. Egypt, 2012.

2012040647

Arab Republic of Egypt. Ministry of state for Scientific Research, Academy of Scientific Research and Technology

 

Low cost

production of natural bacterial secondary metabolite as anticancer agent, Texas Tech University System, USA, 2014, Pending Provisional Patent

 

Therapeutics

and prophylactic effect of natural bacterial secondary metabolite as anticancer agent, Texas Tech University System, USA, 2014, Pending Provisional Patent

 

Novel solvent system for membrane glycoprotein extraction of carcinoma cell lines with different clinicopathological feature, Texas Tech University, 2015, Pending Provisional Patent

 

Flash isolation method of O-glycan, Texas Tech University, 2016,  Provisional patent

 

Chapter in Book”

 

  1. Mechref, Y., Zhou, S., Hu, Y., Hussein, A. and Tang, H. 2014. Quantitative Glycomics by Mass Spectrometry and Liquid Chromatography-Mass Spectrometry. Encyclopedia of Analytical Chemistry. 1–22.
  2. Zhu, E. Song, A. Hussein, F. Kobeissy, Y. Mechref “Glycoproteins Enrichment and LC-MS/MS Glycoproteomics in Central Nervous System Applications” in S. Stevens and F. Kobeissy (ed.), Neuroproteomics, Methods in Molecular Biology book series, published by Humana Press, USA, 2016. in press.

Reviewer:

 

Electrophoresis; Vaccine; the Scientific World Journal; Bio-Resources