Screening of Rabies among Dogs in Kelantan Using Direct Fluorescence Antibody Test (DFAT)

Screening of Rabies Using Direct Fluorescence Antibody Test

  • Dr.Fawzia Shalsh , Ministry Science and Technology, Iraq
  • Imad Ibrahim Ali Al-Sultana Professor of Pathology, Head of Pathology Unit Faculty of Medicine, Lincoln University College, Malaysia.
  • Abdul Rahman Bin Azizc, Professor ,Faculty of Veterinary Medicine, University Malaysia Kelantan.
  • Salahudeen Muhamad Professor ,Faculty of Veterinary Medicine, University Malaysia Kelantan.
Keywords: Direct Fluorescent Antibody Test, rabies, Screening


Inarguably, in several developing and in-transition countries, human are threatened by the occurrence of rabies in domestic dogs and it is posing a major concern. However, the infection of rabies in domestic livestock could definitely have economic consequences. This study aimed to determine the occurrence of rabies among dogs in Kelantan by the use of Direct Fluorescent Antibody Test (DFAT) to determine presence of rabies infection in dogs. The detected rabies cases were 12.5% (1 out of 8 samples). The finding might be inconclusive due to small sample size. It was generally difficult to obtain a satisfactory large number representing high sample size. The positive control used in this research work is made up of inactivated rabies vaccine (Rabisin®) whose antigenic determinant is the viral glycoprotein. It was sufficient to be used although the use of live rabies virus for inoculation in brain tissue smear would be a better option for positive control. The finding from the florescence microscopy examination reveals a good brilliant green fluoresce of high grade (+4) for the positive control. The results of the samples show all negative at conjugate dilution 1:100.


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1- Murphy FA, Gibbs EP, Horzinek MC. Student MJ Veterinary Virology. A Division of Harcourt Brace Company 1999, USA, p: 402. 1999;4.
2- Fenner FJ, Bachmann PA, Gibbs EP. Veterinary virology. Academic Press; 2014 Jun 28.
3- Greene, C.E., The Infectious Disease of Dogs and Cats 4th Ed. 2012. Chapter 20: pp 179.
4- OIE, Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2015, Chapter 2.1.13. Rabies
5- Constantine DG. Rabies transmission by nonbite route. Public health reports. 1962;77(4):287. PMID: 13880956
6- Lahane Sunil R.l, Rautmare S.S.,lGanvir P.T.l and Bhuktar VM. Rabies in Animal Part 1 .2016, Western Regional Disease Diagnostic Laboratory
7- Kaplan, C., Turner, G.S., Warrell, D.A. Rabies The Facts. The Chaucer Press Ltd. 1986. (Richard Clay), Great Britain (Bungay, Suffolk).
8- Ehizibolo DO, Nwosuh CI, Ehizibolo EE, Kia GS. Comparison of the Fluorescent Antibody Test and Direct Microscopic Examination for Rabies Diagnosis at the National Veterinary Research Institute, Vom, Nigeria. African Journal of Biomedical Research. 2009;12(1):73-6.
9- Barrat, J. Experimental Diagnosis of Rabies. Adaptations to Field and Tropical Conditions.Proceedings of the International Conference on Epidemiology, Control and Prevention of Rabies in Eastern and Southern Africa. Lusaka, Zambia, 1992, 72–83.
10- Barton LD, Campbell JB. Measurement of rabies-specific antibodies in carnivores by an enzyme-linked immunosorbent assay. Journal of wildlife diseases. 1988;24(2):246-58.
11- Premalatha BR, Roopa SR, Vijaya M. Immunofluorescence in oral pathology: Part I-methodology. World J Dent. 2011 Oct;2:326-3.
12- Meslin, F.M. (1997) Zoonoses in the world: current and future trends. In Information circular. WHO Mediterranean Zoonoses Control Centre 42.
13- Department of Veterinary Services (2015). Laporan Harian Kejadian Wabak Penyakit Rabies.
14- King, ARabies Diagnosis Manual Southern and Eastern African Rabies Group (SEARG) .2000.
15- Warner CK, Whitfield SG, Fekadu M, Ho H. Procedures for reproducible detection of rabies virus antigen mRNA and genome in situ in formalin-fixed tissues. Journal of virological methods. 1997 Aug 1;67(1):5-12.
16- Ananda RA, Ser HL, Letchumanan V. Updates on the development of vaccines and therapeutic options against rabies. Progress In Microbes & Molecular Biology. 2020 Aug 26;3(1). Doi: 10.36877/pmmb.a0000100
17- Pranee Panichabhongse (2001) The epidemiology of rabies in Thailand. 2001. MVsc Thesis. Massey University, New Zealand. PraneePanichabhongseMVS.pdf (3,390 KB)
How to Cite
Shalsh, D., Al-Sultana, I. I. A., Bin Azizc, , A. R., & Muhamad, S. M. (2021). Screening of Rabies among Dogs in Kelantan Using Direct Fluorescence Antibody Test (DFAT). Al-Qadisiyah Journal of Pure Science, 26(4), 73–79.
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