An investigation of sheep toxoplasmosis in Basrah Province / Iraq

  • K. M. Al-Saad Coll. of Vet. Med. / Univ. of Basrah
  • Saad Hashim Al-Husseiny Coll. of Vet. Med. / Univ. of Al-Qadisiyah http://orcid.org/0000-0002-0966-9515

Abstract

The objective of this study was to investigate Toxoplasma gondii antibodies among sheep in different regions of Basrah province (including Al-Mdayna, Shatt Al-Arab, Al-Basrah, Al-Zubayr, and Abu Al-Khasib). The study was started in Oct. 2008 and was finished in May 2009, using latex agglutination test (LAT) and indirect enzyme linked immunosorbent assay (ELISA) IgG, 309 adult sheep were randomly selected from 15 herds among different ages and both sexes and used in this study, including 62 pregnant ewes, 185 non-pregnant ewes, 14 aborted ewes, and 48 rams. Results showed, that 60.84% were seropositive by LAT, whereas 51.11% were seropositive by ELISA IgG test, among animals used in this study, results detected that 79.03% pregnant ewes (highest value), 56.75% nonpregnant ewes,71.40% aborted ewes and 50% Rams (lowest value) were seropositive by LAT, whereas 56.52% pregnant ewes, 51.11% non-pregnant ewes, 83.33% aborted ewes (highest value), and 31.25% Rams (lowest value) were seropositive by indirect ELISA IgG. Moreover, among regions of Basrah province, the details of percentage of T.gondii antibodies were 54.54% in AL-Basrah , 71.43% in Abu Al-Khasib (highest value), 57.35% in Al- Mdayna, 47.83% in Shatt Al-Arab (lowest value), and 67.16% in Al-Zubayr by LAT, whereas 63.64% in AL-Basrah (highest value), 22.73% in Abu Al-Khasib (lowest value), 57.89% in Al-Mdayna, 50% in Shatt Al-Arab and 61.90% in Al-Zubayr by indirect ELISA test. Although the difference observed in the percentage of Toxoplasma gondii antibodies among different regions of Basrah, there was no significant difference P>0.05 detected LAT, whereas in the indirect ELISA IgG test there was significant difference P<0.05. Ewes showed high percentages 62.83%, 55.40% of toxoplasmosis than rams 50 %, 31.25% by LAT and ELISA test respectively. The highest titer was 1/4 28.57% were detected in pregnant ewes and lowest titers were 1/2, 1/8, and 1/256 0.0% were detected in aborted ewes and in rams
respectively.

References

Dubey, J.P.(1986). Toxoplasmosis. J. A.V. M.A.189: 166-167.
2. Abu Samra, N.(2006). Seroprevalence and zoonotic implication of toxoplasmosis in sheep in South Africa M.Sc. thesis. Faculty of Veterinary Science, University of Pretoria. South Africa
3. Kimbita, E.N., Xuan, X., Huang, X., Miyazawa,T., Fukumoto, S., Mishima, M., Suzuki, H., Sugimoto, C. Nagasawa, H., Fujisaki, K. Suzuki, N., Mikami, T. and Igarashi, I.,(2001). Serodiagnosis of Toxoplasma gondii in a cat by enzyme-linked immunosorbent assay using recombinant SAG1.Vet. Parasitol. 102: 35-44.
4. Barakat, A.M.A.; Abd Elaziz, M. M. and Fadaly, H.A. (2009) Comparative diagnosis of Toxoplasma in Egyptian small ruminants by indirect haemagglutination assay and ELISA .Global Veterinaria, 3(1):9-14.
5. Yousef, A. M. (2005) Some studies on toxoplasmosis in sheep transmissible to man. M.Sc. thesis, Faculty of Medicine, Assiut University, Egypt. (English Abstract).
6. Buxton, D.(1998). Toxoplasma gondii, Neospora caninum, Sarcocystis spp. In sheep and oat. Vet. Res. 24(3-4):289- 310.
7. Esteban–Redondo & Innes, E. A. (1998) . Detection of Toxoplasma gondii in the tissue of sheep orally challenged with a different dose of oocysts. Int. J.Parasitol.28(9): 1459 – 1466.
8. Roberts, L.S. & Janovy, J. (2000). Foundation of Parasitology. 6th ed. Mc Craw-Hill companies, London. pp:127- 132.
9. Schares, G., Vrhovec, M.G., Pantchev, N., Herrmann, D.C. & Conraths, F.J.(2008).An occurrence of Toxoplasma gondii and Hammondia hammondi oocysts in the feces of cats from Germany and other European countries. Vet. Parasitol.152: 34-45.
10. Abd-Al Hameed, A.S.(2007). Seroepidemiological study on ovine toxoplasmosis in Baghdad & Diyala provinces. M.Sc. thesis. Coll. of veterinary medicine university of Baghdad, Iraq. (In Arabic).
11. Al-Attar, 2000. Epidemiological study of toxoplasmosis in Kirkuk city. M.Sc. thesis, college of education, Tikrit University.
12. Preacher, K.J.(2001) Digital interactive Chi-square test program. Uni. Kansas.
13. Buxton, D., Stephan, W. M., Wright, S.E. Rodger, S., Bartley, P. and Innes, A. (2007). Toxoplasma gondii and Ovine Toxoplasmosis: New aspects of an old story. Vet. Parasitol.149:25-28.
14. Gamble, H.R.; Dubey, J.P. & Lambillotte, D.N. (2005). Comparison of commercial ELISA with the modified agglutination test (MAT) for the detection of Toxoplasma infection in domestic pigs. AL-Qadisiyah Journal of Vet.Med.Sci Number of 4th conference 24-25 April 2010 836 Vet. Parasitol. 182(3-4):177- 181.
15. AL – Taee, A.F.M.(2002) Survey on the presence of Toxoplasma gondii antibodies in aborted ewes in Nineveh Province. The Iraqi, J. Vet. Med. Vol. 16 , No. 1 : 9 – 16 . (In Arabic).
16. Mahdii, A. J. (1988). Seroepidemiological study of toxoplasmosis in sheep, M.Sc. thesis, College of Vet. Med. Univ. of Baghdad, Iraq. (In Arabic).
17. Al- Griari, A.J.A. (2007). A seroepidemiological study of toxoplasmosis in Diyala province / Iraq, M.Sc. thesis, college of education, Biology Dept./ Zoology. University of Diyala, Diyala, Iraq. (In Arabic).
18. Negash, T.; Tilahun, G.; Patton, S.; Prevot, F. & Dorchies, P.H.(2004). Serological survey on toxoplasmosis in sheep and goats in Nazareth, Ethiopia, Revue de medecine veterinaire 155(10):486-487.
19. Zain-Eldin, E.A.; Elkhawad, S.E. and Kheir, H.S.M.(1985). A serologic survey of Toxoplasma antibody in cattle, sheep, goats and camel (Camelus dromedaries) inSudan. Revued 'Elevage et de Medecine Veterinaire des
pays Tropicaux 38: 247-249.
20. Al-Sim'ani, R.G.G. (2000). Study of detection & diagnosis of toxoplasmosis in the center of Nineveh governorate. M.Sc. thesis Coll. Vet. Med. University of Mosul, Mosul, Iraq. (In Arabic).
21. Abd-Allah, A.D. (2004). Serological & histological study of Toxoplasma gondii in slaughtered animals and experimental infection in mice. M.S.C. thesis. College of Vet. Med. University of Mosul, Mosul, Iraq. (In Arabic).
22. Mohamed, M.A.W. (2000). climatic features of Basrah Province, Basrah, a cultural encyclopedia of Basrah: 51- 54.
23. AL-Dezzii, S.A.K., and Jwaad, B.A.(2007) Waves of summer humidity in Iraq” study in general climate “ Journal of Arts college. Baghdad University, No(83):387-389(in Arabic).
24. Radostitis, O. M., C.C. Gay, D.C. Blood, and K.W. Hinchliff, (2007). Veterinary Medicine. A textbook of the diseases of cattle, sheep, goats and horses.10th ed, WB saunders Co. London, Newyork, Philadelphia, pp:1518-1522
25. Esteban-Redondo; Maley, S.W., Thomson, K., Nicoll, S., Wright, S., Buxton, D. and Innes, E.A.(1999). Detection of Toxoplasma gondii in tissues of sheep and cattle following oral infection. Vet. Parasitol. 86: 155-171.
26. Nissapaton, V.; Noor Azmi, M.A.; Cho, S.M.; Frong, M.Y.; Init, I.; Rohela, M.; Khairul, A.; Quek, K.F. & Latt, H.M.(2003).Toxoplasmosis: prevalence and risk factors. J. Obstet. Gynecol. 23(6):618- 624
27. Sharma, S., Sandhu, K.S. , Bal, M.S. , Kumar, H., Verma, S. & Dubey, J. P. (2008). Serological survey of antibodies to Toxoplasma gondii in sheep, cattle & buffaloes in Punjab , India. J. Parasitol. 94(5) : 1174 –1175.
28. Quinn, P.J, Carter, M.E., Marky, B .and Carter .G.R. (1999). Clinical veterinary microbiology 1st ed. Elsevier Ldt. London, pp:78-79.
Published
2010-12-30