Phylogenic analysis of the sheep Orf virus in Iraq

  • Salah Mahdi Karim Department of Internal Veterinary Medicine, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq.
  • Mohsen Abd Al- Rodhan College of Pharmacy, University of Al-Qadisiyah, Iraq
  • Khalefa Ali Mansou Department of Internal Veterinary Medicine, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq.
  • Nawras Kadhum Mahdee Department of Internal Veterinary Medicine, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq.
  • Saad Hashim Al-Husseiny Department of Internal Veterinary Medicine, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq. http://orcid.org/0000-0002-0966-9515

Abstract

Orf disease is a highly contagious, zoonotic viral skin disease affects sheep, goats and some other domesticated and wild ruminants, therefor  this study is designed to evaluate predominant genotypes circulating  Orf virus of sheep in Al-Qadisiyah Province in Iraq as the first time by using the sequencing and phylogenic analysis of isolates Orf virus strains. The study was carried out on (94) skin samples of affected sheep animals diagnosed previously by RT-PCR technique in different ages and sexes between September /2013– March/ 2014. PCR assay was performed for amplification of 408bp GM_CSF/IL-2 inhibition factor (GIF) gene Orf virus in positive samples of real-time PCR assay. Eight purified DNA products isolated from different areas of Al-Qadisiyah Province (one sample for each area) were analyzed by using sequencing method to obtain the nucleotide sets of GIF gene. DNA sequencing technique was performed for Phylogenic relationship analysis study of local Orf virus positive samples isolates with NCBI-GenBank Global Orf virus isolates. The results of endpoint polymerase chain reaction for detection of specific GIF gene (408 bp) of Orf virus showed positive results for all collected examined samples. Sequencing analysis of our isolates in the present study recorded and published under accession numbers were (KJ508895.1, KJ634611.1, KJ634842.1, KJ648451.1, KJ653445.1, KJ653446.1, KJ659373.1 and KJ697772.1). The results of phylogeny tree construction showed that five of our published isolates of this study (KJ508895.1, KJ648451.1, KJ653445.1, KJ653446.1, and KJ697772.1) are located in the same first tree branch, which shared highest and closed relationship with AF192803.1, DQ184476.1 Orf virus strain NZ2 isolated from New Zealand. The KJ659373.1 our published Iraqi Orf virus isolates of this study showed high homology and closed relationship with AY605973.1 Orf virus isolated from reindeer in Norway. Phylogenic analysis of KJ634611.1 and KJ634842.1 our published Iraqi Orf isolates of the present study showed high homology with AY605977.1 Orf virus isolated from goat in Norway. Sequencing study and phylogenic analysis have been considered useful in understanding Orf virus scenario in Iraqi endemic area and which important in application of control measures and selective efficient vaccines to use in vaccination programs in Iraq.

References

1-Gokce HI, Genc O, Gokce G.Sero-prevalence of Contagious Ecthyma in Lamb and Humans in Kars, Turkey.Turk. J. Vet. Anim. Sci., (2005); 29: 95-101.
2-Gallina L, Scagliarini L, McInnes CJ, Guercio A, Purpari G, Prosperi S, Scagliarini A. Parapoxvirus in goats: experimental infection and genomic analysis. Veterinary Research Communica-tions, (2008); 32, S203-S205.
3-Mercer AA, Uedaa N, Friederichs S, Hofmann K, Fraser KM, Bateman T, Fleming SB. Comparative analysis of genome sequences of three isolates of Orf virus reveals unexpected sequence variation. Virus Research, (2006); 116:146-158.
4-Lewis C. Update on Orf. In Farm Animal Practice, (1996); 18: 376-381.
5-Fleming SB, McCaughan CA, Andrews AE, Nash AD, Mercer AA. A homolog of interleukin-10 is encoded by the poxvirus Orf virus. J Virol (1997); 71, 4857-4861.
6-Haig DM, Fleming S. Immunomodulation by virulence proteins of the parapoxvirus Orf virus. et Immunol Immunopathol. (1999); 15; 72(1-2):81-6.
7-Seet BT, Johnston JB, Brunetti CR, Barrett JW, Everett H, Cameron C, Sypula J, Nazarian SH, Lucas A, McFadden G. Poxviruses and immune evasion. Annu. Rev. Immunol. (2003a); 21, 377-423.
8-Haig DM, McInnes CJ, Thomson J, Wood A, Bunyan K, Mercer A. The Orf virus OV20.0L gene product is involved in interferon resistance and inhibits an interferon-inducible, double stranded RNA-dependent kinase. Immunology (1998); 93, 335-340.
9-McInnes CJ, Wood AR, Mercer AA. Orf virus encodes a homolog of the vaccinia virus interferon-resistance gene E3L. Virus Genes (1998); 17, 107–115.
10-Deane D, McInnes CJ, Percival A, Wood a, Thomson J, Lear A, Gilray J, Fleming S, Mercer A, Haig D. Orf virus encodes a novel secreted protein inhibitor of granulocyte-macrophage colony-stimulating factor and interleukin-2. J. Virol. (2000);74, 1313-1320.
11-McInnes CJ Deane D, Haig D, Percival A, Thomson J, Wood, AR. Glycosylation, disulfide bond formation, and the presence of a WSXWS-like motif in the Orf virus GIF protein are critical for maintaining the integrity of Binding to ovine granulocytemacrophage colony-stimulating factor and interleukin-2. J. Virol. (2005); 79, 11205-11213.
12-Haig DM, McInnes CJ, Hutchison G, Seow H-F, Reid HW. Cyclosporin-A abrogates the acquired immunity to cutaneous reinfection with the parapoxvirus Orf virus. Immunology (1996); 89:524-531.
13-Strockbine LD, Cohen JI, Farrah T, Lyman SD, Wagener F, DuBose RF, Armitage RJ, Spriggs MK. The Epstein-Barr virus BARF1 gene encodes a novel, soluble colony-stimulating factor-1 receptor. J. Virol. (1998); 72:4015-4021.
14-Espy MJ, Uhl JR, Sloan LM, Buckwalter SP, Jones MF, Vetter EA, Yao JD, Wengenack NL, Rosenblatt JE, Cockerill 3rd FR, Smith TF. Real-time PCR in clinical microbiology: applications for routine laboratory testing. Clin. Microbiol. Rev. 19 (January (1)), (2006); 165-256.
15-Hosamani M, Yadav S, Kallesh DJ, Mondal B, Bhanuprakash V, Singh RK. Isolation and characterization of an Indian ORF virus from goats. Zoonoses Public. Health. (2007) 54, 204-208.
16-Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. (2007); 24, 1596-1599.
17-Goujon M, McWilliam H, Li W, Valentin F, Squizzato S, Paern J, Lopez R. Clustal W2 A new bioinformatics analysis tools framework at EMBL-EBI .Nucleic acids research, (2010); W695-9 doi:10.1093/nar/gkq313.
18-Saitou N, Nei M. The neighbor-joining method: A new method for reconstructing phylogenic trees. Molecular Biology and Evolution (1987); 4:406-425.
19-Tamura K, Nei M, Kumar S. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences (USA) (2004); 101:11030-11035.
20-Felsenstein J. Confidence limits on phylogenies: An approach using the bootstrap. Evolution (1985); 39:783-791.
21-Klein J, Tryland M. Characterisation of parapoxviruses isolated from Norwegian semi-domesticated reindeer (Rangifer tarandus tarandus). Virol. J. (2005); 2, 79.
22-KanouY, asuo Inoshima Y, Shibahara T, Ishikawa Y, Kadota K, Ohashi S, Morioka K, Yoshida K, Yamada S. Isolation and Characterization of a Parapoxvirus from Sheep with Papular Stomatitis. JARQ, (2005); 39 (3), 197-203.
Published
2018-12-30