Rapid Detection of Avian Influenza Virus H9 in broilers by using RT-PCR in Al-Qadisiyah Province

  • Abbas Hadi Jasim Al- Mahmoudi Department of Pathology, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq
  • Nafea S. Jasim Department of Pathology, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq


This study was conducted to detect Avian Influenza Virus H9 in broilers chicken farms in Al-Qadisiyah Province. A tracheal swab from 30 infected chicken flocks located in different areas of Al-Qadisiyah Province was collected to make rapid Immunochromatography test for AIV by using rapid Immunochromatographic  Influenza virus antigen One-Step Kit and tissue samples  were collected from flocks which showed positive for a rapid test to make rRT-PCR. The revealed that twenty-one (70%) flocks were positive for AIV by rapid Immunochromatography test. The molecular detection of AIV showed 23(76.67%) out of 30 flocks were positive for AIV H9.  In conclusion, AIV was the most important cause of respiratory diseases at the time of the study. Both tests were found rapid, efficient and specific to detect AIV, but rt RT-PCR was more sensitive and more specific.


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