Preparation of Organic Seleno cystine Using Locally Isolated Yeast
Abstractselenium is a toxic inorganic form at very low concentration to the life, while theorganic-selenium compound sareappre ciableinterest and various of them haveessentialrolesinnutritionalscienceandcellbiochemistry.Selenium-enrichedyeast(Seyeast)isapublicformofseleniumusedtoadditionaldietaryintakeofthisessentialtracemineral.Inthisstudy,weprepareanorganicselenocystinebyusinglocallyisolatedbakeryyeast(Saccharomycescerevisiae).Anovellocallyprepareddateextractmediaenrichedby0.2%potassiumphosphate(KH2PO4),0.6%ammoniumsulfatewasadoptedasalternativeculturemedia.Seleniumsaltwas used in differentconcentrations(30,60,120and240μg/mL),whichwereaddedtotheyeastculturemedia.Whilethebestconcentrationofseleniumaddedwas30μg/mL,itachievedoptimalconditionsforthegrowthof red coloryea stidentical to the standard.Theorganicselenocystine ereanalyzed by High Performance LiquidChromatography (HPLC) and Atomic Absorption Spectrophotometer (AAS) comparing with standard product obtained from Sigma. Results confirmedtheformationofsimilarselenocystineproducts
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