Preparation of Organic Seleno cystine Using Locally Isolated Yeast

  • Fouad Houssein Kamel Medical Technical Institute, Erbil Polytechnic University ,Erbil

Abstract

selenium is a toxic inorganic form at very low concentration to the life, while theorganic-selenium compound sareappre ciableinterest and various of them haveessentialrolesinnutritionalscienceandcellbiochemistry.Selenium-enrichedyeast(Seyeast)isapublicformofseleniumusedtoadditionaldietaryintakeofthisessentialtracemineral.Inthisstudy,weprepareanorganicselenocystinebyusinglocallyisolatedbakeryyeast(Saccharomycescerevisiae).Anovellocallyprepareddateextractmediaenrichedby0.2%potassiumphosphate(KH2PO4),0.6%ammoniumsulfatewasadoptedasalternativeculturemedia.Seleniumsaltwas used in differentconcentrations(30,60,120and240μg/mL),whichwereaddedtotheyeastculturemedia.Whilethebestconcentrationofseleniumaddedwas30μg/mL,itachievedoptimalconditionsforthegrowthof red coloryea stidentical to the standard.Theorganicselenocystine ereanalyzed by High Performance LiquidChromatography (HPLC) and Atomic Absorption Spectrophotometer (AAS) comparing with standard product obtained from Sigma. Results confirmedtheformationofsimilarselenocystineproducts
Published
2018-05-03
Section
Articles