Detection of Extended Spectrum β-lactamases Producing by Klebsiella pneumonia Isolated from Urinary tract Infection Patients by Using mPCR Technique

  • Wafaa Abdelwahed Jaheel Al-Kaaby College of Biotechnology/ Al-Qadisiyah University
Keywords: K. pneumonia, β-Lactamase, antimicrobial.


Objective: In this study Klebsiella pneumonia where isolated from urine samples and detection of extended spectrum β-Lactamases by using mPCR technique.
Method: This isolated subjected to the antimicrobial susceptibility test (Disc diffusion) for some antibiotics following by multiplex PCR techniques for detection extended spectrum β-Lactamase genes (bla TEM, bla SHV, bla CTX-M and bla AMPC).
Results: The extended spectrum β-Lactamase genes by PCR techniques were given bla CTX-M (30/93.75%), bla SHV (25/78.12%), bla TEM (18/56.25%) and bla AMPC (22/68.15%), respectively.
Conclusion: K. pneumonia isolates of urinary tract infection patients highly associated with the emergence of bla CTX-M β-Lactamase that provides useful good treatment.