Molecular determination of extended spectrum b-lactamases antibiotics resistance genes in E.coli isolated from diarrhea in cattle

  • Ghassan Khudhair Ismaeel Department of Internal Veterinary Medicine, College of Veterinary Medicine, University of
  • Hassan Hachim Nasser Unit of Zoonotic Diseases, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq.

Abstract

   None response to the treatment by an antibiotic called antibiotics resistance result from some genes called resistance genes .This mechanism is widespread in most of the bacteria, like E.coli . All of the extended resistance genes called (ESBIS) is a typical example for study of some genes that resistance beta-lactam antibiotic is subject of this research. Fifty feces sample were collected from cattle suffering from diarrhea in alqaissiyah city were cultured on selective media for E.coli , then DNA was extracted from all  E.coli isolates for antibiotic resistance gene detection by PCR ; The results of this study revealed the prevalence of B-lactamase gene four B-lactamases genes in E.coli blaAmpc gene were (91.4%), the blactx-m gene were (80%), blaTem were (62.8%) and finally and blaSHV gene were (22%) among isolates E.coli ; blaAMPC gene has high prevalence than others genes while blaSHV was a lower percentage than other genes

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Published
2017-11-15
How to Cite
ISMAEEL, Ghassan Khudhair; NASSER, Hassan Hachim. Molecular determination of extended spectrum b-lactamases antibiotics resistance genes in E.coli isolated from diarrhea in cattle. AL-Qadisiyah Journal of Veterinary Medicine Sciences, [S.l.], v. 16, n. 1, p. 67-73, nov. 2017. ISSN 2313-4429. Available at: <http://qu.edu.iq/journalvm/index.php/vm_journal/article/view/38>. Date accessed: 24 june 2018. doi: https://doi.org/10.29079/vol16iss1art38.