Clinical and immunological effects of experimental infection with Klebsiella pneumoniae in lambs in Iraq
The study was conducted on ten Awassi male sheep to study experimentally pathological changes of Klebsiella pneumoniae infection during (3 months). Experimental animals were divided randomly into equal groups(each group included 5 animals), induction of pneumonia in one of these group in sheep by intratracheal route with the infective dose (3 ml) of bacterial suspension in concentration (105 CFU/ml) & the second represent as control and gave a sterile D.W. in the same dose & route of infection) and clinically exam daily with weekly complete blood indices, humoral and cellular immunity were examined by passive hemagglutination test and phagocytic activity as well as histopathological examination of lungs from two scarified sheep in the first group. Nasal discharge appeared as serous then turned to mucopurulent, intermittent cough, mild fever, increase in pulse and respiratory rates (42.4±1.39 and 35.2±1.77 respectively with significant differences between the two groups. WBC count increased significantly in the infected group as compared with the control group. GRN% showed statistically a significant increase in the values reached to (8.88±0.86) during that period of infection compared with control and Mon% showed a statistically significant increase in values during that period in the infected group, and the Abs titration was the higher in the infected group significantly than control and the cellular response in the infected group revealed increment in phagocytic index of neutrophils than in the control group.
2-Mashishi MSK. Respiratory diseases in goat and sheep. Department of agriculture. South Africa. (2007).
3-Azizi Sh, Korani FSh, Oryan A. Pneumonia in slaughtered sheep in southwestern Iran: pathological characteristics and aerobic bacterial aetiology. Vet. Ital., (2013); 49 (1): 109-118.
4-Mansour AMA, zaki HM, Hassan NA, Al-humiany AA. Molecular characterization and immunoprotective activity of capsular polysaccharide of klebsiella pneumoniae isolated from farm animals At Taif governorate. Am. J. Inf. Dis., (2014); 10 (1): 1-14.
5-Seniya Ch, Trivedia SS, Verma R, Vijayarti HS, Vyas Sh, Verma SK. Effect of Fe2+, Mn2+ and Cu2+ ions on growth and soluble protein production in Citrobatcer freundii and Klebsiella pneumoniae. J. Chem. Pharm. Res., (2012); 4(4): 1895-1900.
6-Rashid T, Ebringer A. Ankylosing spondylitis is linked to Klebsiella-the evidence. Clin. Rheumatol., (2007);26(3): 858-864.
7-Lundberg U, Senn BM, Schüler W, Meinke A, Hanner M. Identification and characterization of antigens as vaccine candidates against Klebsiella pneumoniae .Human Vaccines and Immunotherapeutic., (2013);9(3): 497–505.
8-Quinn PJ, Markey BK, Carter ME, Donnelly WJ, Leonard FC. Veterinary Microbiology and Microbial Disease. Blackwell Publishing Company, London. (2002).
9-Boyden SV. Fixation of bacterial products by erythrocytes treated with tannic acid and subsequent heamagglutination by antiproton sera. J. Exp. Med., (1951); 39: 107.
10-Park BH, Fikrig SM, Smith EM. Infection and nitro blue-tetrazolium reduction by neutrophils. Lancet. (1968); 2: 532-534.
11-Smith BP. Ruminant respiratory system. Large Animal Internal Medicine, 4th ed. West line Industrial Drive, St. Louis, Missouri, (2009).
12-Berendt RF, Long GG, Abeles FB, Canonico PG, Elwell MR, Powanda MC. Pathogenesis of respiratory Klebsiella pneumoniae infection in rats: bacteriological and histological findings and metabolic alterations. Infect. Immun., (1977); 15: 586-593.
13-Domenico P, Johanson WGJ, Straus DC. Lobar pneumonia in rats produced by clinical isolates of Klebsiella pneumoniae. Infect. Immun., (1982);37: 327-335.
14-Sharma S, Chhibber S, Mohan H, Sharma S. Induction of Chronic Pneumonia in Normal Mice by Klebsiella pneumoniae. Am. J. Biomed. Sci., (2012); 4(2): 102-110.
15-Scott P. Antibiotic Treatment Response of Chronic Lung Diseases of Adult Sheep in the United Kingdom Based upon Ultrasonographic Findings.Veterinary Medicine International. Hindawi Publishing Corporation, (2014).
16-Nishi T, Tsuchiya K. Experimental respiratory tract infection with klebsiella pneumoniae dt-s in mice: Chemotherapy with Kanamycin Antimicrobial agents and Chemotherapy. (1980); 17(3): 494-505.
17-Held TK, Trautmann M, Mielke MEA, Neudeck H, Jr SJC, Cross AS Monoclonal antibody against Klebsiella capsular polysaccharide reduces severity and hematogenic spread of experimental Klebsiella pneumoniae pneumonia. Inf. and immun., (1992); 60(5): 1771-1778.
18-Saleh NS, Allam TS. Pneumonia in Sheep: Bacteriological and Clinicopathological Studies. Am. J.Res. Commun. (2014); 2 (11): 70-88.
19-Wieland CW, Van Lieshout MHP, Hoogendijk AJ, Van der Poll T. Host defense during Klebsiella pneumonia relies on haematopoietic-expressed Toll-like receptors 4 and 2. Eur. Respir. J., (2011); 37: 848-857.
20-ORUC E. The Pathologic and Bacteriologic Comparison of Pneumonia in Lambs. Turk. J. Vet. Anim. Sci., (2006); 30: 593-599.
21-El-Naser EMA, Khamis GFA. Some hematological and blood serum biochemistry associated with respiratory affections in camels. Assiut. Vet. Med. J., (2009); 55(123): 154-162.
23-Chhibber S, Wadhwa S, Yadav V. Protective role of liposome incorporated lipopolysaccharide antigen of Klebsiella pneumoniae in a Rat model of lober pneumonia. Jpn. J. Infect. Dis., (2004); 57: 150-155.
24-Thomas EL, Greisham MB, Jefferson MM. Myeloperoxidase dependent effect of amines on function of isolated neutrophils. Am. Soci. Clin. Inves., (1983);72: 441-454.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright: All manuscripts published by Al-Qadisiyah Journal of Veterinary Medicine Sciences are under the terms of the Creative Commons Attribution License. This permits anyone to copy, distribute, transmit and adapt the work provided the original work and source is appropriately cited. Copyright on any open access article published by Al-Qadisiyah Journal of Veterinary Medicine Sciences is retained by the author(s).
Note: The journal is not allow any scientific misconduct in any published research article. Any article exceeds the permissible ratio of plagiarism is rejected. However, as a publisher will follow strict scientific guidelines and advice to retract or erratum of any article at any time if found scientific misconduct or errors in any articles. Authors are responsible for obtaining permission from copyright holders for reproducing any illustrations, tables, figures or lengthy quotations previously published elsewhere.