Clinical, microbial, histopathological and molecular investigation of interstitial pneumonia in camels in Iraq

  • Muthanna Hadi Hussain Department of Internal Veterinary Medicine, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq.
  • Faisal G. Habasha Department of Internal Veterinary Medicine, College of Veterinary Medicine, Baghdad University, Iraq.
  • Kalefa Ali Mansour Department of Internal Veterinary Medicine, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq.

Abstract

In this study, twenty-four camels in abattoirs of Al-Najaf and Al-Qadisiyah provinces were suspected to have interstitial bronchopneumonia. Clinical signs revealed protracted neck, misery, inappetence, heart rate was elevated (44±0.29bpm) and breathing was irregular and rapid (23±0.27bpm) and fever (39.2±0.1). Moist crackles heard at auscultation. Transtracheal wash (TTW) were sampled for cytology and bacteriology to make the diagnosis; white blood cells count (WBC) was (1420±5.95cells/µl) with neutrophilia (48%), total protein (TP) records (355±4.29 mg/dl). Bacterial culture of the TTW revealed pure colonies on blood agar; which were recognized by the VITEK 2 compact device and confirmed using the conventional polymerase chain reaction (PCR) as Klebsiella pneumonia ssp pneumonia. Postmortem specimen gave three kinds of colonies: the same one in TTW, which was the causative pathogen, two others; Staphylococcus lentus and St. vitulinus; diagnosed biochemically by VITEK 2 compact. Histopathological dissections on postmortem samples found in the lungs of camels ranged only in 4-8 years old, discovered the presence of interstitial bronchopneumonia.

References

1-Fowler ME, Bravo PW. Medicine and surgery of Camelids, respiratory system. 3rd ed. Blackwell publishing's, USA. (2010).
2-Al-Tarazi YH. Bacteriological and pathological study on pneumonia in the one humped camel (camelus dromedarius) in Jordan Revue Élev. Méd. Vét. Pays trop., (2001); 54 (2): 93-97.
3-Arora RG, Kalra DS. A note on isolation of Klebsiella pneumoniae and Diplococci from cases of broncho-pneumonia in camels. Ind. J. Anim. Sci., (1973);43 (12), 1095-1096.
4-Elmossalami E, A Ghawi. Public health importance of camel lung affections. Egypt. J. Vet. Sci. (1981);18 (1-2): 109-119.
5-Rana MZ, Ahmed A, Sindhu ST, Mohammad G. Bacteriology of camel lungs. Camel Newsletter. (1993); 10, 30-32.
6-Amer HA, Omnia FH, Salem SA. Diagnosis of Some Respiratory Diseases in Camel Calves. Minufiya Veterinary Journal, (2002); Vol. 2. No. 1.
7-Abubakar MS, Fatihu MY, Ibrahim ND, Oladele SB. Camel pneumonia in Nigeria: Epidemiology and bacterial flora in normal and diseased lung. African Journal of Microbiology Research Vol. (2010); 4(23), p:2479-2483.
8-Manaa AM, Abdel-All Th S. Studies Affect Cases of Bacterial Respiratory Infection on Some Biochemical Changes in Camels at Assiut Governorate. Assiut Veterinary Medical Journal, Assiut Veterinary Medical Journal. Vol. 57, No. 129 (2011); pp. 145-158.
9-Radostits OM, Joe Mayhew IG, Houston DM. Veterinary clinical examination anddiagnosis, clinical examination of the respiratory tract. Elsevier limited, china. (2005).
10-Bradford P S. Large animal internal medicine, 4th ed., Diseases of the Respiratory System. West line Industrial Drive, St. Louis, Missouri, (2009).
11-Harold EA, David PA, Sir JA, Franklin ML. The Merck veterinary manual, 8th ed., Clinical pathology and procedures. Merck and Co., INC., Whitehouse, USA. (2000).
12-Pinglei LA, Peng LA, Xiaofei JB, Dexi BA, Yingzhou XA, Cui TA, Zixin DA, Kumar RC, Hong UO. Complete Genome Sequence of Klebsiella pneumoniae subsp. pneumoniae HS11286, a Multidrug-Resistant Strain Isolated from Human Sputum. J. Bacteriol. (2012); 194(7):1841
13-Sylvain B, Francine G, Patrick ADG. The Genus Klebsiella. Prokaryotes (2006); 6:159–196
14-Boye K, Hansen DS. Sequencing of 16S rDNA of Klebsiella: taxonomic relations within the genus and to other Enterobacteriaceae. Int. J. Med. Microbiol. (2003);292 (7-8), 495-503.
15-Peter GG, Peter DC. Clinical Examination of Farm Animals, Clinical Examination of the Respiratory System, Ch 7. Blackwell Science, Inc., 350 Main Street, Malden, MA02148-5018, USA, (2002).
16-Fowler ME, Bravo PW. Medicine and surgery of Camelids, Anesthesia. 3rd ed. Blackwell publishing's, USA, (2010).
17-Morag GK. Veterinary laboratory medicine, clinical biochemistry and haematology, chapter 4, the plasma protein. 2nd ed.. Blackwell Science Ltd. Oxford, London, (2002).
18-Markus RW, Aaron ZF. A manual for biochemistry protocols, protein analysis. World Scientific Publishing Co. Pte. Ltd. Singapore. (2007).
19-Amstutz HE, Anderson DP, Benzel HA. The Merck veterinary manual, bovine respiratory disease complex, 8th ed. By Merk and co. INC. pan - American copyright convention, (2000).
20-Meyer DJ, Harvey JW. Veterinary laboratory medicine: interpretation and diagnosis.3rd ed., Saunders, Elsevier Inc, USA. (2004).
21-Ronald MA. Handbook of microbiological media. 3rd ed.. CRC PRESS, LLC, 2000 N.W. Corporate Blvd., Boca Raton, Florida 33431. (2004).
22-David HP. Microbial identification using the BioMérieux VITEK® 2 System. BioMérieux, Inc. Hazelwood, MO, USA. (2009).
23-Jang SS, Biberstein EL. Manual of veterinary clinical bacteriology and mycology. (1981).
24-Bancroft JD, Stevens A. Theory practice of histological examination. 2nd edition, New York. (1982); Pp: 166-190.
25-Bioneer Corporation, 8-11, Munpyeongseo-ro Daedeok-gu, Daejeon 306-220. South Korea. Order no. A20140324-058.
26-Habasha FG, Muthanna Hadi Hussain. Cytological analysis of transtracheal washes from healthy camels in Al-Diwaniya province. Al-Qadissiya Journal for Vet. Med. Sci. No.1Vol.13, Iraq, Al-Qadissiya. (2013).
27-Leech NL, Barrett KC, Morgan GA. IBM SPSS for Intermediate statistics.4thed. Taylor and Francis Group. LLC. USA. (2011).
28-Nourani H, Rohani F. Pathological Study on Lungs of Dromedary Camels Slaughtered in the Central Part of Iran. Journal of Camel Practice and Research. (2009); Vol. 16 No. I, p 51-54.
29-Ganti AS, Rama PR. Veterinary pathology, the respiratory system. 7th ed. J.S. Offset Printers, (2008); Delhi- 110051.
30-Al-Tarazi YH. Bacteriological and pathological study on pneumonia in the one humped camel (camelus dromedarius) in Jordan Revue Élev. Méd. Vét. Pays trop., (2001); 54 (2): 93-97.
31-Samuel TB. Gross and microscopic pulmonary lesions of camels from Eastern Ethiopia. Trop Anim Health Prod (2008); 40:25–28.
32-Richard D. Study on pathology of dromedary in Borena Awraja (Ethiopia). Doctor of Veterinary Thesis (DVM). Maisons Alfort. IEMVT, France. (1979).
Published
2018-06-28
How to Cite
HUSSAIN, Muthanna Hadi; HABASHA, Faisal G.; MANSOUR, Kalefa Ali. Clinical, microbial, histopathological and molecular investigation of interstitial pneumonia in camels in Iraq. Al-Qadisiyah Journal of Veterinary Medicine Sciences, [S.l.], v. 17, n. 1, p. 132-137, june 2018. ISSN 2313-4429. Available at: <http://qu.edu.iq/journalvm/index.php/vm_journal/article/view/488>. Date accessed: 10 dec. 2018. doi: https://doi.org/10.29079/vol17iss1art488.