Identification of Campylobacter jejuni in poultry products by Real-Time PCR in Al-Muthanna province

  • Muna Tawfeeq Abd Coll. of Vet. Med. / Univ. of Al-Qadisiyah
  • Huda AbdAlhadei Al-Nasrawi Coll. of Vet. Med. / Univ. of Al-Qadisiyah

Abstract

The study was undertaken due to the potential contribution of poultry products is less well known in Iraq. In this study, Campylobacter jejuni identified by application SYBR green-based Real-Time PCR technique by amplification the specific hipo gene in (96) chicken tissue samples. The results show no significant difference in detection rates between fresh (77.8%) and frozen (83.3%) poultry tissue samples. In freshly slaughtered poultry samples, the liver detection rate (93.75 %) was higher than skin (62.5%) and meat (75%) while in frozen poultry samples, the detection rate was higher in meat (93.75 %) than skin (87.5%) and liver (68.75%). In conclusion, the study showed high contamination rates of poultry with Campylobacter jejuni in retail markets and the direct Real-Time PCR is a suitable assay for screening poultry products and identification of Campylobacter jejuni pathogen

Published
2015-12-30