Evaluation of the virucidal efficacy of commercial antiviral drugs against Lasota virus a surrogate for enveloped viruses
Many antivirals are commonly used in Iraqâ€™s poultry farms and there is controversy about effective of these commercial antiviral drugs. The aim of this study was tested individually for the effectiveness of these commercial antiviral drugs. Four kinds of commercial antiviral drugs including VIRUXÂ®, TopAMDÂ®, V8Â® and LicoriceÂ® was used in this study. Lasota virus was used as a surrogate for enveloped viruses. The following tests: Spot hemagglutination, Antigen Rapid NDV Ag Test Kit, Electron microscopy, qRTPCR and Egg inoculation in order to determine the effect of these antivirals on hemagglutinating activity, a viral protein, viral morphology, virus titer and viral infectivity respectively. All antivirals had no toxic effect on the chicken embryos. All these antivirals had no effect on haemagglutination activity except Licorice. No antivirals changed nucleoprotein antigenicity of Lasota virus. All antivirals had no effect on the morphology of the virus except Licorice destroyed the viral morphology and decreased in viral spikes. Three of the four antiviral reduced the viral titer while Licorice complete degradation of viral RNA and prevent detection it by qRTPCR. The allantoic fluid harvested from inoculated eggs with the treated Lasota virus with antivirals showed a remarkable decrease in viral infectivity as following: TopAMP about 20%, V8 40%, and Virux 60% while Licorice showed a complete reduction of viral infectivity (100%). In conclusion, the Licorice revealed the best antiviral activity.
2-Bhella D, Ralph A, Murphy LB, Yeo RP. Significant differences in nucleocapsid morphology within the Paramyxoviridae. J Gen Virol (2002);83, 1831â€“1839.
3-Bieker JM, Souza CA, Oberst RD â€œInactivation of Various Influenza Strains to Model Avian Influenza (Bird Flu) With Various Disinfectant Chemistries,â€ Sandia National Laboratory, Albuquerque, New Mexico 87185 and Livermore, California 94550. (2005).
4-Brian WJ Mahy, Hillar O Kangro. Virology methods manual. US Edition. San Diego: Academic press. (1996).
5-Chadare FJ, Linnemann AR, Hounhouigan JD, Nout, MJR, Van Boekel MAJ. Baobab Food Products: A Review on their Composition and Nutritional Value. Crit. Rev. Food Sci. Nutr. (2009);49: 254-274.
6-Charrel RN, de Chesse RA, Decaudin P, De Micco, de Lamballerie X. â€œEvaluation of disinfectant efficacy against hepatitis C virus using RT-PCR-based methodâ€ J Hosp Infect, (2001);49, pp.129â€“134.
7-Connaris H, Takimoto T, Russell R, Crennell S, Moustafa I, Portner A, Taylor G. Probing the sialic acid binding site of the hemagglutinin-neuraminidase of Newcastle disease virus: identification of key amino acids involved in cell binding, catalysis, and fusion. J Virol (2002);76, 1816â€“1824.
8-Elizondo-Gonzalez R, Cruz-Suarez LE, Ricque-Marie D, Mendoza-Gamboa E, Rodriguez-Padilla C, Trejo-Avila LM. In vitro characterization of the antiviral activity of fucoidan from Cladosiphon okamuranus against Newcastle Disease Virus. Virol J (2012);9, 307.
9-Eterpi M, McDonnell G, Thomas V. Disinfection efficacy against parvoviruses in comparison to reference viruses. Journal of Hospital Infection, (2009);73, 64-70.
10-Jang J, Sung-Hwan H, Ik-Hwan K. Validation of a Real-Time RT-PCR Method to Quantify Newcastle Disease Virus (NDV) Titer and Comparison with Other Quantifiable Methods. J. Microbiol. Biotechnol., (2011); 21(1), 100â€“108.
11-Jawetz E, Melnick LJ, Adelberg EA, Brooks GF. Medical Microbiology (26th ed) Appleton and Lange, London. (1998) ; ISBN-13: 978-0071790314.
12-Jeffrey DJ. Chemicals used as disinfectants: active ingredients and enhancing additives. Rev Sci Tech, (1995);14:57-74.
13-Ma J, Straub TM, Pepper IL, Gerba CP. â€œCell culture and PCR determination of Poliovirus inactivation by disinfectants,â€ Appl. Environ. Microbiol, 60, (1994);pp.4203â€“4206.
14-McDonnell G. Antisepsis, disinfection and sterilization: Types, action and resistance. Washington, DC: ASM Press. (2007).
15-McDonnell G, Russell AD. Antiseptics and disinfectants: Activity, action, and resistance. Clinical Microbiology Reviews, (1999);12,147-179.
16-Murakawa Y, Sakaguchi K, Soejima K, Eriguchi S, Takase K, Sueyoshi M, Nagatomo H, Ito T, Otuski K. Heamagglutinating activity of the lentogenic Newcastle disease virus strain MET95. Avian Pathol. (2003);32: 39-45.
17-Ojeh CK, Cusack TM, Yolken RH. â€œEvaluation of the effects of disinfectants on rotavirus RNA and infectivity by the polymerase chain reaction and cell culture methods, â€œMol Cell Probes, 9, (1995);pp.341â€“346.
18-Suarez DL, Spackman E, Senne DA, Bulaga L, Welsch AC, Froberg K. The effect of various disinfectants on detection of avian influenza virus by real time RT-PCR. Avian Dis. (2003);47: 1091-1095.
19-Swayne DE, Senne DA, Beard CW. Avian influenza. Pages 150â€“155 in A Laboratory Manual for the Isolation and Identification of Avian Pathogens. 4th ed. (1998).
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