Recovering cultivation-based identification of Escherichia coli from poultry in Al-Diwaniyah, Iraq

  • Lubna Abd Al-Raheem Shtiwey Department of pathology and poultry disease, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq.
  • Nafea Sabeeh Jasim Department of pathology and poultry disease, College of Veterinary Medicine, University of Al-Qadisiyah, Iraq.


Aiming at Escherichia coli-based identification, the current work was intended to recover this microorganism from chickens affected by avian pathogenic Escherichia coli (APEC). To perform this an investigation from July to November -2015, 200 chicken-gut-based specimens were collected from different locations in Al-Diwaniyah City, Iraq. The affected chickens first investigated for macroscopic lesions such as on the external surface of the lungs that belonged to the affected birds. Gut-related parts such as duodenum suffered characteristic features of inflammation-based signs. Then, samples were cultivated on media agars for recovering APEC, detecting of infection rate, identifying active antimicrobial agents for better treatment. The sick chickens suffered various health problems such as intestinal inflammation and air sacculitis. Using MacConkey, TBX agar, and API-20E, the results showed the presence of these pathogenic microorganisms in the infected parts of these chickens.


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